VIM-1-producing Enterobacter asburiae with mobile colistin resistance genes from wastewaters.
mcr
Antimicrobial resistance
Carbapenemase
Environment
Journal
BMC genomics
ISSN: 1471-2164
Titre abrégé: BMC Genomics
Pays: England
ID NLM: 100965258
Informations de publication
Date de publication:
17 Sep 2024
17 Sep 2024
Historique:
received:
09
03
2024
accepted:
06
09
2024
medline:
20
9
2024
pubmed:
20
9
2024
entrez:
19
9
2024
Statut:
epublish
Résumé
Wastewaters are considered as important players in the spread of antimicrobial resistance, thus affecting the health of humans and animals. Here, we focused on wastewaters as a possible source of carbapenemase-producing Enterobacterales for the environment. A total of 180 presumptive coliforms from hospital and municipal wastewaters, and a river in the Czech Republic were obtained by selective cultivation on meropenem-supplemented media and tested for presence of carbapenemase-encoding genes by PCR. Strains carrying genes of interest were characterized by testing antimicrobial susceptibility, carbapenemase production and combination of short- and long- read whole-genome sequencing. The phylogenetic tree including publicly available genomes of Enterobacter asburiae was conducted using Prokka, Roary and RAxML. Three VIM-producing Enterobacter asburiae isolates, members of the Enterobacter cloacae complex, were detected from hospital and municipal wastewaters, and the river. The bla Our findings pointed out the important contribution of hospital and community wastewaters in transmission of multi-drug resistant pathogens.
Sections du résumé
BACKGROUND
BACKGROUND
Wastewaters are considered as important players in the spread of antimicrobial resistance, thus affecting the health of humans and animals. Here, we focused on wastewaters as a possible source of carbapenemase-producing Enterobacterales for the environment.
METHODS
METHODS
A total of 180 presumptive coliforms from hospital and municipal wastewaters, and a river in the Czech Republic were obtained by selective cultivation on meropenem-supplemented media and tested for presence of carbapenemase-encoding genes by PCR. Strains carrying genes of interest were characterized by testing antimicrobial susceptibility, carbapenemase production and combination of short- and long- read whole-genome sequencing. The phylogenetic tree including publicly available genomes of Enterobacter asburiae was conducted using Prokka, Roary and RAxML.
RESULTS
RESULTS
Three VIM-producing Enterobacter asburiae isolates, members of the Enterobacter cloacae complex, were detected from hospital and municipal wastewaters, and the river. The bla
CONCLUSION
CONCLUSIONS
Our findings pointed out the important contribution of hospital and community wastewaters in transmission of multi-drug resistant pathogens.
Identifiants
pubmed: 39300338
doi: 10.1186/s12864-024-10780-7
pii: 10.1186/s12864-024-10780-7
doi:
Substances chimiques
Wastewater
0
beta-Lactamases
EC 3.5.2.6
Colistin
Z67X93HJG1
VIM-1 metallo-beta-lactamase
EC 3.5.2.-
Anti-Bacterial Agents
0
Bacterial Proteins
0
carbapenemase
EC 3.5.2.6
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
870Subventions
Organisme : Ministerstvo Zdravotnictví Ceské Republiky
ID : NU20J-09-00040
Organisme : Internal Grant Agency of the University of Veterinary Sciences Brno
ID : 208/2023/FVHE
Informations de copyright
© 2024. The Author(s).
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