Development of a µPAD for the point-of-care testing of serum glutamic oxaloacetic transaminase (SGOT).
Colourimetric detection
Fast Blue BB salt
ImageJ software
Liver function biomarker
Paper analytical devices
Point-of-care testing
RGB values
Serum glutamic oxaloacetic transaminase
Journal
Mikrochimica acta
ISSN: 1436-5073
Titre abrégé: Mikrochim Acta
Pays: Austria
ID NLM: 7808782
Informations de publication
Date de publication:
25 Sep 2024
25 Sep 2024
Historique:
received:
12
06
2024
accepted:
02
09
2024
medline:
25
9
2024
pubmed:
25
9
2024
entrez:
25
9
2024
Statut:
epublish
Résumé
A wax-patterned paper analytical device (µPAD) has been developed for point-of-care colourimetric testing of serum glutamic oxaloacetic transaminase (SGOT). The detection method was based on the transamination reaction of aspartate with α-ketoglutarate, leading to the formation of oxaloacetate which reacts with the reagent Fast Blue BB salt and forms a cavern pink colour. The intensity of the cavern pink colour grows as the concentration of SGOT increases. UV-visible spectroscopy was utilized to optimize reaction conditions, and the optimized reagents were dropped onto the wax-patterned paper. The coloured PADs, after the addition of SGOT, have been photographed, and a colour band has been generated to correlate the SGOT concentration visually. The images were used to calculate the intensity values using ImageJ software, which inturn was used to calculate the SGOT concentration. The PADs were also tested with serum samples, and SGOT spiked serum samples. The PAD could detect the SGOT concentration ranging from 5 to 200 U/L. The analysis yielded highly accurate results with less than 6% relative error compared to the clinical sample. This colourimetric test demonstrated exceptional selectivity in the presence of other biomolecules in the blood serum, with a detection limit of 2.77 U/L and a limit of quantification of 9.25 U/L. Additionally, a plasma separation membrane was integrated with the PAD to directly test SGOT from finger-prick blood samples.
Identifiants
pubmed: 39320528
doi: 10.1007/s00604-024-06678-6
pii: 10.1007/s00604-024-06678-6
doi:
Substances chimiques
Aspartate Aminotransferases
EC 2.6.1.1
Ketoglutaric Acids
0
Aspartic Acid
30KYC7MIAI
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
619Subventions
Organisme : Department of Biotechnology (DBT), India
ID : Sanction no. 102/IFD/SAN/2238/2016-17 dated 30/8/2016, and 102/IFD/SAN/1555/2018-2019 dated 13/6/2018.
Organisme : Department of Biotechnology (DBT), India
ID : Sanction no. 102/IFD/SAN/2238/2016-17 dated 30/8/2016, and 102/IFD/SAN/1555/2018-2019 dated 13/6/2018.
Organisme : Department of Biotechnology (DBT), India
ID : Sanction no. 102/IFD/SAN/2238/2016-17 dated 30/8/2016, and 102/IFD/SAN/1555/2018-2019 dated 13/6/2018.
Organisme : Department of Biotechnology (DBT), India
ID : Sanction no. 102/IFD/SAN/2238/2016-17 dated 30/8/2016, and 102/IFD/SAN/1555/2018-2019 dated 13/6/2018.
Organisme : Department of Biotechnology (DBT), India
ID : Sanction no. 102/IFD/SAN/2238/2016-17 dated 30/8/2016, and 102/IFD/SAN/1555/2018-2019 dated 13/6/2018.
Organisme : Department of Biotechnology (DBT), India
ID : Sanction no. 102/IFD/SAN/2238/2016-17 dated 30/8/2016, and 102/IFD/SAN/1555/2018-2019 dated 13/6/2018.
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.
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