Low protein diet protects the liver from Salmonella Typhimurium-mediated injury by modulating the mTOR/autophagy axis in macrophages.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
30 Sep 2024
30 Sep 2024
Historique:
received:
12
03
2024
accepted:
20
09
2024
medline:
1
10
2024
pubmed:
1
10
2024
entrez:
30
9
2024
Statut:
epublish
Résumé
Western diets are the underlying cause of metabolic and liver diseases. Recent trend to limit the consumption of protein-rich animal products has become more prominent. This dietary change entails decreased protein consumption; however, it is still unknown how this affects innate immunity. Here, we studied the influence of a low protein diet (LPD) on the liver response to bacterial infection in mice. We found that LPD protects from Salmonella enterica serovar Typhimurium (S. Typhimurium)-induced liver damage. Bulk and single-cell RNA sequencing of murine liver cells showed reduced inflammation and upregulation of autophagy-related genes in myeloid cells in mice fed with LPD after S. Typhimurium infection. Mechanistically, we found reduced activation of the mammalian target of rapamycin (mTOR) pathway, whilst increased phagocytosis and activation of autophagy in LPD-programmed macrophages. We confirmed these observations in phagocytosis and mTOR activation in metabolically programmed human peripheral blood monocyte-derived macrophages. Together, our results support the causal role of dietary components on the fitness of the immune system.
Identifiants
pubmed: 39349819
doi: 10.1038/s42003-024-06932-w
pii: 10.1038/s42003-024-06932-w
doi:
Substances chimiques
TOR Serine-Threonine Kinases
EC 2.7.11.1
mTOR protein, mouse
EC 2.7.1.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1219Subventions
Organisme : RCUK | Biotechnology and Biological Sciences Research Council (BBSRC)
ID : BB/R012490/1
Organisme : RCUK | Biotechnology and Biological Sciences Research Council (BBSRC)
ID : BBS/E/F/000PR10355
Organisme : RCUK | Biotechnology and Biological Sciences Research Council (BBSRC)
ID : BB/CCG1860/1
Organisme : RCUK | Biotechnology and Biological Sciences Research Council (BBSRC)
ID : BB/X011054/1
Organisme : RCUK | Biotechnology and Biological Sciences Research Council (BBSRC)
ID : BBS/E/F/000PR13632
Organisme : RCUK | Biotechnology and Biological Sciences Research Council (BBSRC)
ID : BB/X011011/1
Organisme : RCUK | Biotechnology and Biological Sciences Research Council (BBSRC)
ID : BBS/E/F/000PR13634
Organisme : RCUK | Biotechnology and Biological Sciences Research Council (BBSRC)
ID : BB/W002450/1
Organisme : RCUK | Biotechnology and Biological Sciences Research Council (BBSRC)
ID : BB/CCG1720/1
Organisme : RCUK | Biotechnology and Biological Sciences Research Council (BBSRC)
ID : BBX011070/1
Organisme : RCUK | Biotechnology and Biological Sciences Research Council (BBSRC)
ID : BB/T008717/1
Organisme : RCUK | Biotechnology and Biological Sciences Research Council (BBSRC)
ID : BB/P022073/1
Organisme : Wellcome Trust (Wellcome)
ID : 213731/Z/18/Z
Organisme : Wellcome Trust (Wellcome)
ID : 220534/Z/20/Z
Organisme : RCUK | Medical Research Council (MRC)
ID : MR/T02934X/1
Informations de copyright
© 2024. The Author(s).
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