Metabolic adaptations of Escherichia coli to extended zinc exposure: insights into tricarboxylic acid cycle and trehalose synthesis.
Escherichia coli
/ metabolism
Trehalose
/ metabolism
Citric Acid Cycle
/ drug effects
Zinc
/ metabolism
Oxidative Stress
Escherichia coli Proteins
/ metabolism
Zinc Oxide
/ metabolism
Proteomics
Gene Expression Regulation, Bacterial
/ drug effects
Adaptation, Physiological
Transcriptome
Gene Expression Profiling
Metabolic Networks and Pathways
/ drug effects
Carbohydrate metabolism
Nanoparticles
Proteome
Transcriptome
Trehalose synthesis
Tricarboxylic acid cycle
Virulence
Zinc
Zinc oxide
Journal
BMC microbiology
ISSN: 1471-2180
Titre abrégé: BMC Microbiol
Pays: England
ID NLM: 100966981
Informations de publication
Date de publication:
01 Oct 2024
01 Oct 2024
Historique:
received:
28
05
2024
accepted:
16
08
2024
medline:
2
10
2024
pubmed:
2
10
2024
entrez:
1
10
2024
Statut:
epublish
Résumé
Balanced bacterial metabolism is essential for cell homeostasis and growth and can be impacted by various stress factors. In particular, bacteria exposed to metals, including the nanoparticle form, can significantly alter their metabolic processes. It is known that the extensive and intensive use of food and feed supplements, including zinc, in human and animal nutrition alters the intestinal microbiota and this may negatively impact the health of the host. This study examines the effects of zinc (zinc oxide and zinc oxide nanoparticles) on key metabolic pathways of Escherichia coli. Transcriptomic and proteomic analyses along with quantification of intermediates of tricarboxylic acid (TCA) were employed to monitor and study the bacterial responses. Multi-omics analysis revealed that extended zinc exposure induced mainly oxidative stress and elevated expression/production of enzymes of carbohydrate metabolism, especially enzymes for synthesis of trehalose. After the zinc withdrawal, E. coli metabolism returned to a baseline state. These findings shed light on the alteration of TCA and on importance of trehalose synthesis in metal-induced stress and its broader implications for bacterial metabolism and defense and consequently for the balance and health of the human and animal microbiome.
Identifiants
pubmed: 39354342
doi: 10.1186/s12866-024-03463-6
pii: 10.1186/s12866-024-03463-6
doi:
Substances chimiques
Trehalose
B8WCK70T7I
Zinc
J41CSQ7QDS
Escherichia coli Proteins
0
Zinc Oxide
SOI2LOH54Z
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
384Subventions
Organisme : ERDF "Multidisciplinary research to increase application potential of nanomaterials in agricultural practice" (No. CZ.03.1.01/0.0/0.0/16_025/0007314)
ID : CZ.03.1.01/0.0/0.0/16_025/0007314
Organisme : ERDF "Multidisciplinary research to increase application potential of nanomaterials in agricultural practice" (No. CZ.03.1.01/0.0/0.0/16_025/0007314)
ID : CZ.03.1.01/0.0/0.0/16_025/0007314
Organisme : ERDF "Multidisciplinary research to increase application potential of nanomaterials in agricultural practice" (No. CZ.03.1.01/0.0/0.0/16_025/0007314)
ID : CZ.03.1.01/0.0/0.0/16_025/0007314
Organisme : ERDF "Multidisciplinary research to increase application potential of nanomaterials in agricultural practice" (No. CZ.03.1.01/0.0/0.0/16_025/0007314)
ID : CZ.03.1.01/0.0/0.0/16_025/0007314
Organisme : ERDF "Multidisciplinary research to increase application potential of nanomaterials in agricultural practice" (No. CZ.03.1.01/0.0/0.0/16_025/0007314)
ID : CZ.03.1.01/0.0/0.0/16_025/0007314
Organisme : ERDF "Multidisciplinary research to increase application potential of nanomaterials in agricultural practice" (No. CZ.03.1.01/0.0/0.0/16_025/0007314)
ID : CZ.03.1.01/0.0/0.0/16_025/0007314
Organisme : ERDF "Multidisciplinary research to increase application potential of nanomaterials in agricultural practice" (No. CZ.03.1.01/0.0/0.0/16_025/0007314)
ID : CZ.03.1.01/0.0/0.0/16_025/0007314
Informations de copyright
© 2024. The Author(s).
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