Next generation preservation solution using synthetic enzymes added to polyhemoglobin to protect warm ischemic human hepatocytes and cardiomyocytes.
Humans
Hepatocytes
/ drug effects
Myocytes, Cardiac
/ drug effects
Hemoglobins
/ pharmacology
Cell Survival
/ drug effects
Organ Preservation Solutions
/ pharmacology
Oxidative Stress
/ drug effects
Catalase
/ metabolism
Warm Ischemia
Superoxide Dismutase
/ metabolism
Carbonic Anhydrases
/ metabolism
Cells, Cultured
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
04 Oct 2024
04 Oct 2024
Historique:
received:
12
06
2024
accepted:
22
09
2024
medline:
5
10
2024
pubmed:
5
10
2024
entrez:
4
10
2024
Statut:
epublish
Résumé
This study investigates the potential improvement of polyhemoglobin's protective properties by the addition of 3 synthetic enzymes (neo-carbonic anhydrase, neo-catalase and neo-superoxide dismutase) to polyhemoglobin after 90 and 180 min of warm in-vitro ischemia (100% Nitrogen at 37 °C). Following the warm ischemic shock, cell cultures were subjected to various treatment solutions: Controls; PolyHb; 3 neoenzymes; PolyHb + 3 neoenzymes; PolyHb + 2 neoenzymes. The cultures were then incubated (Oxygen, 5% CO
Identifiants
pubmed: 39367025
doi: 10.1038/s41598-024-73862-2
pii: 10.1038/s41598-024-73862-2
doi:
Substances chimiques
Hemoglobins
0
polyhemoglobin
0
Organ Preservation Solutions
0
Catalase
EC 1.11.1.6
Superoxide Dismutase
EC 1.15.1.1
Carbonic Anhydrases
EC 4.2.1.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
23104Subventions
Organisme : McGill University Advancement Program with unrestricted donation from Mr. Zhang Yi of Proheme
ID : Fund id: McGill account 172810
Organisme : McGill University Advancement Program with unrestricted donation from Mr. Zhang Yi of Proheme
ID : Fund id: McGill account 172810
Informations de copyright
© 2024. The Author(s).
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