Evidence for SARS-CoV-2 infected Golden Syrian hamsters (Mesocricetus auratus) reducing daily energy expenditure and body core temperature.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
06 10 2024
Historique:
received: 12 12 2023
accepted: 20 09 2024
medline: 7 10 2024
pubmed: 7 10 2024
entrez: 6 10 2024
Statut: epublish

Résumé

Golden Syrian hamsters (Mesocricetus auratus) are a well-established animal model for human infections with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) due to their susceptibility to SARS-CoV-2 infection, robust virus replication and pathological manifestations similar to human COVID-19 pneumonia. To investigate the physiological changes upon infection in this animal model, we explored the alterations in daily energy expenditure (DEE), water turnover, body mass, body temperature, and locomotor activity in non-infected and SARS-CoV-2 infected Golden Syrian hamsters for four days post SARS-CoV-2. DEE was measured using the doubly labelled water method, which allows for the accurate estimation of carbon dioxide production and, consequently, energy expenditure in animals. Additionally, we investigated total water intake (TWI), which comprises drinking water, preformed water in food, and metabolic water. Using intraperitoneally implanted data loggers, we also monitored body core temperature and locomotor activity in some of the animals. Here we provide evidence for infected hamsters exhibiting significantly lower DEE and TWI compared to non-infected animals. We also observed an increase in body weight in the non-infected animals, while infected animals experienced weight loss. Further, infected animals showed a significantly decreased body temperature, indicating a generally lowered metabolic rate.

Identifiants

pubmed: 39370428
doi: 10.1038/s41598-024-73765-2
pii: 10.1038/s41598-024-73765-2
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

23263

Informations de copyright

© 2024. The Author(s).

Références

Sumbria, D., Berber, E., Mathayan, M. & Rouse, B. T. Virus infections and host metabolism-can we manage the interactions? Front. Immunol. 11, 594963 (2020).
doi: 10.3389/fimmu.2020.594963 pubmed: 33613518
Moore, J. B. & June, C. H. Cytokine release syndrome in severe COVID-19. Science 368, 473–474 (2020).
doi: 10.1126/science.abb8925 pubmed: 32303591
Que, Y. F. et al. Cytokine release syndrome in COVID-19: a major mechanism of morbidity and mortality. Int. Rev. Immunol. 41, 217–230 (2022).
doi: 10.1080/08830185.2021.1884248 pubmed: 33616462
Anker, M. S. et al. Weight loss, malnutrition, and cachexia in COVID-19: facts and numbers. J. Cachexia Sarcopeni 12, 9–13 (2021).
doi: 10.1002/jcsm.12674
Morley, J. E., Kalantar-Zadeh, K. & Anker, S. D. COVID-19: a major cause of cachexia and sarcopenia? J. Cachexia Sarcopeni 11, 863–865 (2020).
doi: 10.1002/jcsm.12589
Soares, M. N. et al. Skeletal muscle alterations in patients with acute Covid-19 and post-acute sequelae of Covid-19. J. Cachexia Sarcopenia Muscle 13, 11–22 (2022).
doi: 10.1002/jcsm.12896 pubmed: 34997689
Sia, S. F. et al. Pathogenesis and transmission of SARS-CoV-2 in golden hamsters. Nature 583, 834–838 (2020).
doi: 10.1038/s41586-020-2342-5 pubmed: 32408338 pmcid: 7394720
Blaurock, C. et al. Compellingly high SARS-CoV-2 susceptibility of Golden Syrian hamsters suggests multiple zoonotic infections of pet hamsters during the COVID-19 pandemic. Sci. Rep.-Uk 12, (2022).
Harden, L. M., Kent, S., Pittman, Q. J. & Roth, J. Fever and sickness behavior: friend or foe? Brain Behav. Immun. 50, 322–333 (2015).
doi: 10.1016/j.bbi.2015.07.012 pubmed: 26187566
Xiang, H. X. et al. Renal dysfunction and prognosis of COVID-19 patients: a hospital-based retrospective cohort study. BMC Infect. Dis. 21, (2021).
Klein, M. S., Conn, C. A. & Kluger, M. J. Behavioral thermoregulation in mice inoculated with influenza-virus. Physiol. Behav. 52, 1133–1139 (1992).
doi: 10.1016/0031-9384(92)90472-E pubmed: 1484872
Chatfield, P. O. & Lyman, C. P. Circulatory changes during process of arousal in the hibernating hamster. Am. J. Physiol. 163, 566–574 (1950).
doi: 10.1152/ajplegacy.1950.163.3.566 pubmed: 14799632
Jansky, L., Haddad, G., Kahlerova, Z. & Nedoma, J. Effect of external factors on hibernation of Golden-Hamsters. J. Comp. Physiol. 154, 427–433 (1984).
doi: 10.1007/BF00684450
Fatteh, N. et al. Association of hypothermia with increased mortality rate in SARS-CoV-2 infection. Int. J. Infect. Dis. 108, 167–170 (2021).
doi: 10.1016/j.ijid.2021.05.031 pubmed: 34004328 pmcid: 8123521
Musacchia, X. J. Comparative physiological and biochemical aspects of Hypothermia as a model for hibernation. Cryobiology 21, 583–592 (1984).
doi: 10.1016/0011-2240(84)90219-0 pubmed: 6518801
Chayama, Y., Ando, L., Tamura, Y., Miura, M. & Yamaguchi, Y. Decreases in body temperature and body mass constitute pre-hibernation remodelling in the Syrian golden hamster, a facultative mammalian hibernator. R. Soc. Open Sci. 3 (2016).
Stegmann, K. M. et al. Inhibitors of dihydroorotate dehydrogenase cooperate with molnupiravir and N4-hydroxycytidine to suppress SARS-CoV-2 replication. iScience 25, 104293 (2022).
doi: 10.1016/j.isci.2022.104293 pubmed: 35492218 pmcid: 9035612
Wolfel, R. et al. Virological assessment of hospitalized patients with COVID-2019. Nature 581, 465–469 (2020).
doi: 10.1038/s41586-020-2196-x pubmed: 32235945
Lifson, N. Theory of use of the turnover rates of body water for measuring energy and material balance. J. Theor. Biol. 12, 46–74 (1966).
doi: 10.1016/0022-5193(66)90185-8 pubmed: 6015431
Speakman, J. R. Doubly Labelled Water: Theory and Practice (1997).
Riek, A. et al. Energy expenditure and body temperature variations in llamas living in the high Andes of Peru. Sci. Rep.-Uk 9 (2019).
Riek, A., Petow, S., Speakman, J. R. & Schrader, L. Daily energy expenditure and water turnover in two breeds of laying hens kept in floor housing. Animal 15, (2021).
Oftedal, O. T., Hintz, H. F. & Schryver, H. F. Lactation in the horse - milk-composition and intake by foals. J. Nutr. 113, 2096–2106 (1983).
doi: 10.1093/jn/113.10.2096 pubmed: 6619986

Auteurs

Björn-Patrick Mohl (BP)

Institute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany.

Claudia Blaurock (C)

Institute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany.

Alexander Riek (A)

Institute of Animal Welfare and Animal Husbandry, Friedrich-Loeffler-Institut, Celle, Germany.

Catherine Hambly (C)

School of Biological Sciences, University of Aberdeen, Aberdeen, UK.

John R Speakman (JR)

School of Biological Sciences, University of Aberdeen, Aberdeen, UK.

Anne Balkema-Buschmann (A)

Institute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany. Anne.Balkema-Buschmann@fli.de.

Articles similaires

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female
Humans United States Aged Cross-Sectional Studies Medicare Part C
1.00
Humans Yoga Low Back Pain Female Male
Humans Meals Time Factors Female Adult

Classifications MeSH