Structure of a Rhs effector clade domain provides mechanistic insights into type VI secretion system toxin delivery.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
08 Oct 2024
08 Oct 2024
Historique:
received:
18
11
2023
accepted:
26
09
2024
medline:
9
10
2024
pubmed:
9
10
2024
entrez:
8
10
2024
Statut:
epublish
Résumé
The type VI secretion system (T6SS) is a molecular machine utilised by many Gram-negative bacteria to deliver antibacterial toxins into adjacent cells. Here we present the structure of Tse15, a T6SS Rhs effector from the nosocomial pathogen Acinetobacter baumannii. Tse15 forms a triple layered β-cocoon Rhs domain with an N-terminal α-helical clade domain and an unfolded C-terminal toxin domain inside the Rhs cage. Tse15 is cleaved into three domains, through independent auto-cleavage events involving aspartyl protease activity for toxin self-cleavage and a nucleophilic glutamic acid for N-terminal clade cleavage. Proteomic analyses identified that significantly more peptides from the N-terminal clade and toxin domains were secreted than from the Rhs cage, suggesting toxin delivery often occurs without the cage. We propose the clade domain acts as an internal chaperone to mediate toxin tethering to the T6SS machinery. Conservation of the clade domain in other Gram-negative bacteria suggests this may be a common mechanism for delivery.
Identifiants
pubmed: 39379370
doi: 10.1038/s41467-024-52950-x
pii: 10.1038/s41467-024-52950-x
doi:
Substances chimiques
Type VI Secretion Systems
0
Bacterial Toxins
0
Bacterial Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
8709Subventions
Organisme : Department of Health | National Health and Medical Research Council (NHMRC)
ID : 1128981
Organisme : Department of Health | National Health and Medical Research Council (NHMRC)
ID : 1165036
Organisme : Department of Health | National Health and Medical Research Council (NHMRC)
ID : 1165036
Organisme : Department of Health | National Health and Medical Research Council (NHMRC)
ID : 1128981
Organisme : Department of Health | National Health and Medical Research Council (NHMRC)
ID : 1165036
Organisme : Department of Health | National Health and Medical Research Council (NHMRC)
ID : 1128981
Informations de copyright
© 2024. The Author(s).
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