A liquid static culture using a gas-permeable film bag contributes to microbiology.
Escherichia coli
Biofilm
Methylotrophic culture of Komagataella phaffii
Shear stress
TPX
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
10 10 2024
10 10 2024
Historique:
received:
03
08
2024
accepted:
30
09
2024
medline:
10
10
2024
pubmed:
10
10
2024
entrez:
9
10
2024
Statut:
epublish
Résumé
We propose a simple tool for liquid static culture using a copolymer film with high gas permeability. The film bags were successfully used to culture microorganisms Escherichia coli, Komagataella phaffii (methylotrophic) and Bacillus sp. (biofilm-forming), with cells cultured under physical stress-free conditions with sufficient oxygen supply. Similar growth curves and plasmid productivity were observed for liquid shake and film bag E. coli cultures. The early growth response of the film bag culture following colony inoculation of liquid media differed from conventional shake cultures. Our results indicate that a gas-permeable film bag is a promising liquid culture tool and provides novel microbiology materials.
Identifiants
pubmed: 39384930
doi: 10.1038/s41598-024-74954-9
pii: 10.1038/s41598-024-74954-9
doi:
Substances chimiques
Gases
0
Culture Media
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
23649Informations de copyright
© 2024. The Author(s).
Références
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