Enzyme-linked ImmunoSpot (ELISpot) assay to quantify peptide-specific IFN-γ production by splenocytes in a mouse tumor model after radiation therapy.

Animals Mice Interferon-gamma CD8-Positive T-Lymphocytes / immunology Spleen / immunology Enzyme-Linked Immunospot Assay / methods Peptides / immunology Disease Models, Animal
ELISpot Mouse Radiation Splenocytes T cells Tumor antigen

Journal

Methods in cell biology
ISSN: 0091-679X
Titre abrégé: Methods Cell Biol
Pays: United States
ID NLM: 0373334

Informations de publication

Date de publication:
2024
Historique:
medline: 12 10 2024
pubmed: 12 10 2024
entrez: 11 10 2024
Statut: ppublish

Résumé

To develop new effective therapeutic strategies for cancer patients, there is a need for extensive and precise insights into the mechanisms involved in the immune response to anti-cancer treatments. The enzyme-linked immunospot (ELISpot) assay is a rapid and reproducible technique that allows the detection of cytokine-producing antigen-specific T cells at the single cell level. This protocol describes an interferon gamma (IFN-γ) ELISpot method for measuring antigen-specific murine CD8

Identifiants

DOI: 10.1016/bs.mcb.2024.07.001 PMID: 39393885
pubmed: 39393885
pii: S0091-679X(24)00173-0
doi: 10.1016/bs.mcb.2024.07.001
pii:
doi:

Substances chimiques

Interferon-gamma 82115-62-6
Peptides 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

41-54

Informations de copyright

Copyright © 2024 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.

Auteurs

Benoît Lecoester (B)

INSERM, EFS BFC, UMR 1098, RIGHT, Greffon-Hôte-Tumeur Interactions/Ingénierie Cellulaire et Génique, Université de Franche-Comté, Besançon, France.

Yaoyao Xie (Y)

INSERM, EFS BFC, UMR 1098, RIGHT, Greffon-Hôte-Tumeur Interactions/Ingénierie Cellulaire et Génique, Université de Franche-Comté, Besançon, France.

Amélie Marguier (A)

INSERM, EFS BFC, UMR 1098, RIGHT, Greffon-Hôte-Tumeur Interactions/Ingénierie Cellulaire et Génique, Université de Franche-Comté, Besançon, France.

Laura Boulerot (L)

INSERM, EFS BFC, UMR 1098, RIGHT, Greffon-Hôte-Tumeur Interactions/Ingénierie Cellulaire et Génique, Université de Franche-Comté, Besançon, France.

Marine Malfroy (M)

INSERM, EFS BFC, UMR 1098, RIGHT, Greffon-Hôte-Tumeur Interactions/Ingénierie Cellulaire et Génique, Université de Franche-Comté, Besançon, France.

Olivier Adotévi (O)

INSERM, EFS BFC, UMR 1098, RIGHT, Greffon-Hôte-Tumeur Interactions/Ingénierie Cellulaire et Génique, Université de Franche-Comté, Besançon, France; Department of Medical Oncology, University Hospital of Besançon, Besançon, France.

Jihane Boustani (J)

INSERM, EFS BFC, UMR 1098, RIGHT, Greffon-Hôte-Tumeur Interactions/Ingénierie Cellulaire et Génique, Université de Franche-Comté, Besançon, France; Department of Radiation Oncology, University Hospital of Besançon, Besançon, France. Electronic address: jboustani@chu-besancon.fr.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Enzyme-linked ImmunoSpot (ELISpot) assay to...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Enzyme-linked ImmunoSpot (ELISpot) assay to...
questionsmedicales.fr › Facteurs biologiques › Protéines et peptides de signalisation intercellulaire › Cytokines › Lymphokines › Facteurs d'activation des macrophages › Interféron gamma › Enzyme-linked ImmunoSpot (ELISpot) assay to...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Leucocytes › Agranulocytes › Lymphocytes › Lymphocytes T › Lymphocytes T CD8+ › Enzyme-linked ImmunoSpot (ELISpot) assay to...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Système lymphatique › Tissu lymphoïde › Rate › Enzyme-linked ImmunoSpot (ELISpot) assay to...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Dosage immunologique › Techniques d'immunoadsorption › Test ELISA › Test ELISpot › Enzyme-linked ImmunoSpot (ELISpot) assay to...
questionsmedicales.fr › Acides aminés, peptides et protéines › Peptides › Enzyme-linked ImmunoSpot (ELISpot) assay to...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles biologiques › Modèles animaux de maladie humaine › Enzyme-linked ImmunoSpot (ELISpot) assay to...