Immunohistochemical Expression of MDM2, Bcl-2, SATB2 and Ki-67 in Histological Variants of Unicystic Ameloblastoma.
Humans
Matrix Attachment Region Binding Proteins
/ biosynthesis
Proto-Oncogene Proteins c-mdm2
/ metabolism
Female
Ki-67 Antigen
/ metabolism
Male
Proto-Oncogene Proteins c-bcl-2
/ metabolism
Ameloblastoma
/ pathology
Adult
Adolescent
Immunohistochemistry
Biomarkers, Tumor
/ analysis
Young Adult
Child
Transcription Factors
/ metabolism
Jaw Neoplasms
/ pathology
Middle Aged
Bcl-2
Immunohistochemistry
MDM2
Unicystic Ameloblastoma
Journal
Head and neck pathology
ISSN: 1936-0568
Titre abrégé: Head Neck Pathol
Pays: United States
ID NLM: 101304010
Informations de publication
Date de publication:
15 Oct 2024
15 Oct 2024
Historique:
received:
06
08
2024
accepted:
13
09
2024
medline:
15
10
2024
pubmed:
15
10
2024
entrez:
15
10
2024
Statut:
epublish
Résumé
To characterize the immunohistochemical expression of MDM2, Bcl-2, SATB2 and Ki-67 in histological variants of unicystic ameloblastoma (UA). Following the ethical approval, forty (40) patients with unicystic ameloblastoma were retrieved from the archives and subjected to immunohistochemistry (IHC). Sociodemographic and clinical data were also retrieved. The results were entered into a Microsoft Excel spreadsheet and analyzed using SPSS software. Human tooth germs, which served as the control, showed moderate expression of Bcl-2 and MDM2 with slight proliferative activity in ameloblasts and moderate expression of SATB2 in ectomesenchyme and odontoblasts. Luminal UA (Type 1) showed low Ki-67 index and negative to mild Bcl-2 and MDM2 expression, whilst Type 1.2 (luminal and intraluminal), Type 1.2.3 (luminal, intraluminal and mural), and Type 1.3 (luminal and mural), including the recurrent cases, showed moderate to intense expression with high mean Ki-67 index. The difference between the study groups was statistically significant (p value < 0.001). No expression of SATB2 was noted in any histological variant of UA. Furthermore, no significant differences were noted in age, gender, site and location between the groups. In contrast to luminal variant of UA, mural±intraluminal variants and recurrent cases demonstrate higher expression of Bcl-2 and MDM2 with higher mean Ki-67 index. It may thus be prudent to provide aggressive treatment for cases, not just with mural follicles but also for the patients with intraluminal plexiform proliferation, to prevent recurrence and improve patient outcomes.
Identifiants
pubmed: 39404986
doi: 10.1007/s12105-024-01705-7
pii: 10.1007/s12105-024-01705-7
doi:
Substances chimiques
Matrix Attachment Region Binding Proteins
0
SATB2 protein, human
0
Proto-Oncogene Proteins c-mdm2
EC 2.3.2.27
Ki-67 Antigen
0
MDM2 protein, human
EC 2.3.2.27
Proto-Oncogene Proteins c-bcl-2
0
Biomarkers, Tumor
0
Transcription Factors
0
BCL2 protein, human
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
100Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
Références
Ackermann GL, Altini M, Shear M (1988) The unicystic ameloblastoma: a clinicopathological study of 57 cases. J Oral Pathol 17(9–10):541–546. https://doi.org/10.1111/j.1600-0714.1988.tb01331.x
doi: 10.1111/j.1600-0714.1988.tb01331.x
pubmed: 3150441
Philipsen HP, Reichart PA (1998) Unicystic ameloblastoma. A review of 193 cases from the literature. Oral Oncol 34(5):317–325. https://doi.org/10.1016/s1368-8375(98)00012-8
doi: 10.1016/s1368-8375(98)00012-8
pubmed: 9861335
Anand R, Narwal A, Pandiar D, Kamboj M, Devi A, Saxena S et al (2021) Clinicopathological Profile of 80 cases of Unicystic Ameloblastoma aided by a histopathological comparison using modified Philipsen-Reichart classification and Marx-Stern classification. Head Neck Pathol 15(3):875–881. https://doi.org/10.1007/s12105-021-01307-7
doi: 10.1007/s12105-021-01307-7
pubmed: 33638761
pmcid: 8384931
Escobar E, Gómez-Valenzuela F, Peñafiel C, Chimenos-Küstner E, Pérez-Tomás R (2023) Aberrant immunoexpression of p53 tumour-suppressor and Bcl-2 family proteins (Bcl-2 and Bax) in ameloblastomas and odontogenic keratocysts. J Clin Exp Dent 15(2):e125–e134. https://doi.org/10.4317/jced.59769
doi: 10.4317/jced.59769
pubmed: 36911151
pmcid: 9994657
Kim JY, Kim J, Bazarsad S, Cha IH, Cho SW, Kim J (2019) Bcl-2 is a prognostic marker and its silencing inhibits recurrence in ameloblastomas. Oral Dis 25(4):1158–1168. https://doi.org/10.1111/odi.13070
doi: 10.1111/odi.13070
pubmed: 30801855
Tosios KI, Kalogirou EM, Koutlas IG (2024) Association of MDM2 overexpression in Ameloblastomas with MDM2 amplification and BRAFV600E expression. Int J Mol Sci 25(4):2238. https://doi.org/10.3390/ijms25042238
doi: 10.3390/ijms25042238
pubmed: 38396916
pmcid: 10889355
Piattelli A, Lezzi G, Fioroni M, Santinelli A, Rubini C (2002) Ki-67 expression in dentigerous cysts, unicystic ameloblastomas, and ameloblastomas arising from dental cysts. J Endod 28(2):55–58. https://doi.org/10.1097/00004770-200202000-00001
doi: 10.1097/00004770-200202000-00001
pubmed: 11833688
Sandra F, Mitsuyasu T, Nakamura N, Shiratsuchi Y, Ohishi M (2001) Immunohistochemical evaluation of PCNA and Ki-67 in ameloblastoma. Oral Oncol 37(2):193–198. https://doi.org/10.1016/s1368-8375(00)00079-8
doi: 10.1016/s1368-8375(00)00079-8
pubmed: 11167148
Krishna A, Kaveri H, Naveen Kumar RK, Kumaraswamy KL, Shylaja S, Murthy S (2012 Apr-Jun) Overexpression of MDM2 protein in ameloblastomas as compared to adenomatoid odontogenic tumor. J Cancer Res Ther 8(2):232–237. https://doi.org/10.4103/0973-1482.98976
Chen Q, Zheng L, Zhang Y, Huang X, Wang F, Li S et al (2021) Special AT-rich sequence-binding protein 2 (Satb2) synergizes with Bmp9 and is essential for osteo/odontogenic differentiation of mouse incisor mesenchymal stem cells. Cell Prolif 54(4):e13016. https://doi.org/10.1111/cpr.13016
doi: 10.1111/cpr.13016
pubmed: 33660290
pmcid: 8016638
Nevoránková P, Šulcová M, Kavková M, Zimčík D, Balková SM, Peléšková K et al (2024) Region-specific gene expression profiling of early mouse mandible uncovered SATB2 as a key molecule for teeth patterning. Sci Rep 14(1):18212. https://doi.org/10.1038/s41598-024-68016-3
doi: 10.1038/s41598-024-68016-3
pubmed: 39107332
pmcid: 11303781
Xin T, Li Q, Bai R, Zhang T, Zhou Y, Zhang Y et al (2021) A novel mutation of SATB2 inhibits odontogenesis of human dental pulp stem cells through Wnt/β-catenin signaling pathway. Stem Cell Res Ther 12(1):595. https://doi.org/10.1186/s13287-021-02660-8
doi: 10.1186/s13287-021-02660-8
pubmed: 34863303
pmcid: 8642962
Pandiar D, Shameena P (2014) Immunohistochemical expression of CD34 and basic fibroblast growth factor (bFGF) in oral submucous fibrosis. J Oral Maxillofac Pathol 18(2):155–161. https://doi.org/10.4103/0973-029X.140718
doi: 10.4103/0973-029X.140718
pubmed: 25328292
pmcid: 4196280
Krishnan RP, Pandiar D, Sagar S (2024) Immunohistochemical expression of CK14 and Bcl-2 in Odontogenic Keratocyst and its variants. Appl Immunohistochem Mol Morphol 32(3):151–156. https://doi.org/10.1097/PAI.0000000000001182
doi: 10.1097/PAI.0000000000001182
pubmed: 38146077
Pandiar D, Krishnan RP (2024) Gingival Carcinoma Cuniculatum: a Case Series of seven cases with emphasis on immunohistochemical and histochemical features. Indian J Otolaryngol Head Neck Surg 76(3):2930–2938. https://doi.org/10.1007/s12070-024-04559-1
doi: 10.1007/s12070-024-04559-1
pubmed: 38883458
Sindura C, Babu C, Mysorekar V, Kumar V (2013) Study of immunohistochemical demonstration of Bcl-2 protein in ameloblastoma and keratocystic odontogenic tumor. J Oral Maxillofac Pathol 17(2):176–180. https://doi.org/10.4103/0973-029X.119750
doi: 10.4103/0973-029X.119750
pubmed: 24250074
pmcid: 3830222
Li TJ, Wu YT, Yu SF, Yu GY (2000) Unicystic ameloblastoma: a clinicopathologic study of 33 Chinese patients. Am J Surg Pathol 24:1385–1392
doi: 10.1097/00000478-200010000-00008
pubmed: 11023100
Vered M, Wright JM (2022) Update from the 5th Edition of the World Health Organization Classification of Head and Neck tumors: odontogenic and maxillofacial bone tumours. Head Neck Pathol 16(1):63–75. https://doi.org/10.1007/s12105-021-01404-7
doi: 10.1007/s12105-021-01404-7
pubmed: 35312978
pmcid: 9019005
Reichart PA, Philipsen HP, Sonner S (1995) Ameloblastoma: biological profile of 3677 cases. Eur J Cancer B Oral Oncol 31B(2):86–99. https://doi.org/10.1016/0964-1955(94)00037-5
doi: 10.1016/0964-1955(94)00037-5
pubmed: 7633291
Dodge OG (1965) Tumors of the jaw, odontogenic tissues and maxillary antrum (excluding Burkitt lymphoma) in Uganda africans. Cancer 18(2):205–215
doi: 10.1002/1097-0142(196502)18:2<205::AID-CNCR2820180212>3.0.CO;2-G
pubmed: 14254077
Rosenstein T, Pogrel MA, Smith RA, Regezi JA (2001) Cystic ameloblastoma- behavior and treatment of 21 cases. J Oral Maxillofac Surg 59:1311–1316
doi: 10.1053/joms.2001.27522
pubmed: 11688034
Carreón-Burciaga RG, González-González R, Molina-Frechero N, Bologna-Molina R (2015) Immunoexpression of Ki-67, MCM2, and MCM3 in Ameloblastoma and Ameloblastic Carcinoma and their correlations with clinical and histopathological patterns. Dis Markers 2015:683087. https://doi.org/10.1155/2015/683087
doi: 10.1155/2015/683087
pubmed: 26823641
pmcid: 4707386
Scholzen T, Gerdes J (2000) The Ki-67 protein: from the known and the unknown. J Cell Physiol 182(3):311–322. https://doi.org/10.1002/(SICI)1097-4652(200003)182:3%3C;311::AID-JCP1%3E;3.0.CO;2-9
doi: 10.1002/(SICI)1097-4652(200003)182:3<311::AID-JCP1>3.0.CO;2-9
pubmed: 10653597
Jabbarzadeh M, Hamblin MR, Pournaghi-Azar F, Vakili Saatloo M, Kouhsoltani M, Vahed N (2021 Winter) Ki-67 expression as a diagnostic biomarker in odontogenic cysts and tumors: a systematic review and meta-analysis. J Dent Res Dent Clin Dent Prospects 15(1):66–75. https://doi.org/10.34172/joddd.2021.012
Olimid DA, Florescu AM, Cernea D, Georgescu CC, Mărgăritescu C, Simionescu CE et al (2014) The evaluation of p16 and Ki67 immunoexpression in ameloblastomas. Rom J Morphol Embryol 55(2):363–367
pubmed: 24969987
Apellániz D, Pereira-Prado V, Sicco E, Vigil-Bastitta G, González-González R, Mosqueda-Taylor A et al (2018) Comparative study of the Minichromosome Maintenance Proteins Complex (MCM 4/5/6) in Ameloblastoma and Unicystic Ameloblastoma. Int J Surg Pathol 26(8):714–720. https://doi.org/10.1177/1066896918778341
doi: 10.1177/1066896918778341
pubmed: 29845895
Scott J, Adams C, Simmons K, Feather A, Jones J, Hartzell L et al (2018) Dental radiographic findings in 18 individuals with SATB2-associated syndrome. Clin Oral Investig 22(8):2947–2951. https://doi.org/10.1007/s00784-018-2702-9
doi: 10.1007/s00784-018-2702-9
pubmed: 30315422
Pandiar D, Ramani P, Krishnan RP, Thamilselvan S, Ramya R (2021) Dysplastic epithelial changes in odontogenic keratocyst: a rare histological presentation with immunohistochemical cognizance. Oral Oncol 122:105580. https://doi.org/10.1016/j.oraloncology.2021.105580
doi: 10.1016/j.oraloncology.2021.105580
pubmed: 34689009
Honma N, Horii R, Ito Y, Saji S, Younes M, Iwase T, Akiyama F (2015) Differences in clinical importance of Bcl-2 in breast cancer according to hormone receptors status or adjuvant endocrine therapy. BMC Cancer 15:698. https://doi.org/10.1186/s12885-015-1686-y
doi: 10.1186/s12885-015-1686-y
pubmed: 26472348
pmcid: 4607008
Cho IC, Chung HS, Cho KS, Kim JE, Joung JY, Seo HK, Chung J, Park WS, Hong EK, Lee KH (2010) Bcl-2 as a predictive factor for biochemical recurrence after radical prostatectomy: an interim analysis. Cancer Res Treat 42(3):157–162. https://doi.org/10.4143/crt.2010.42.3.157
doi: 10.4143/crt.2010.42.3.157
pubmed: 20948921
pmcid: 2953779
Manusirivithaya S, Siriaunkgul S, Khunamornpong S, Sripramote M, Sampatanukul P, Tangjitgamol S, Srisomboon J (2006) Association between Bcl-2 expression and tumor recurrence in cervical cancer: a matched case-control study. Gynecol Oncol 102(2):263–269. https://doi.org/10.1016/j.ygyno.2005.12.037
doi: 10.1016/j.ygyno.2005.12.037
pubmed: 16483641
Vieira Costa R, Balbinot KM, da Silveira GCAR, Kataoka MSDS, de Menezes SAF, Freitas VM et al (2024) Prognostic value of the expression and localization of cell proliferation and apoptosis markers in unicystic ameloblastomas. Sci Rep 14(1):3856. https://doi.org/10.1038/s41598-024-54132-7
doi: 10.1038/s41598-024-54132-7
pubmed: 38360984
pmcid: 10869795