Aptamer-guided graphene oxide quantum dots for targeted suicide gene therapy in an organoid model of luminal breast cancer.
Humans
Graphite
/ chemistry
Quantum Dots
/ chemistry
Female
Breast Neoplasms
/ genetics
Genetic Therapy
/ methods
Aptamers, Nucleotide
/ genetics
Organoids
/ metabolism
Genes, Transgenic, Suicide
MCF-7 Cells
Caspase 9
/ metabolism
Polyethyleneimine
/ chemistry
Gene Transfer Techniques
Mucin-1
/ genetics
Gene delivery
Graphen oxide quantum dot (GOQD)
Luminal breast cancer
Organoid model
Suicide gene therapy
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
15 10 2024
15 10 2024
Historique:
received:
18
03
2024
accepted:
25
09
2024
medline:
16
10
2024
pubmed:
16
10
2024
entrez:
15
10
2024
Statut:
epublish
Résumé
Breast cancer is one of the most common cancers in women. One of the best therapeutic methods against breast cancer is gene therapy, while having an appropriate gene carrier is the biggest challenge of gene therapy. Hence, developing carriers with low cytotoxicity and high gene transfection efficiency, and preferentially with the selective function of gene delivery is a critical demand for this method. In the present study, we introduce a novel targeted carrier to deliver the inducible caspase-9 suicide gene (pLVSIN-iC9) into breast cancer cells. The carrier is composed of graphene oxide quantum dots decorated with polyethyleneimine, and S2.2; an aptamer with high affinity to MUC1 (GOQD-PEI/S2.2). Due to the overexpression of MUC1 in breast cancer cells, the designed GOQD-PEI/S2.2/pLVSIN-iC9 can selectively target cancer cells. Moreover, to better mimic solid tumor conditions, and to evaluate the selective effect of the GOQD-PEI/S2.2/pLVSIN-iC9, an organoid model derived from human dermal fibroblasts (HDF) and MCF-7 cells (coculture organoid) was generated and characterized. The results demonstrate that the coculture organoid model adapts the tissue structure of luminal breast cancer, as well. Therefore, the organoids were subjected to treatment with targeted gene therapy using GOQD-PEI/S2.2/pLVSIN-iC9. Our evidence supports the targeted killing effect of iC9 on the breast cancer cells of the organoids and suggests the good potential of the newly introduced carriers in targeted gene delivery.
Identifiants
pubmed: 39406784
doi: 10.1038/s41598-024-74312-9
pii: 10.1038/s41598-024-74312-9
doi:
Substances chimiques
Graphite
7782-42-5
graphene oxide
0
Aptamers, Nucleotide
0
Caspase 9
EC 3.4.22.-
Polyethyleneimine
9002-98-6
Mucin-1
0
MUC1 protein, human
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
24104Subventions
Organisme : Iran National Science Foundation
ID : 4026873
Informations de copyright
© 2024. The Author(s).
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