Effects of Helicobacter pylori and Helicobacter pylori eradication on the microbiota of tongue coating.
Humans
Helicobacter pylori
/ drug effects
Helicobacter Infections
/ microbiology
Male
Female
Tongue
/ microbiology
Adult
Middle Aged
RNA, Ribosomal, 16S
/ genetics
Bacteria
/ classification
Microbiota
/ drug effects
Anti-Bacterial Agents
/ pharmacology
Young Adult
DNA, Bacterial
/ genetics
Phylogeny
Eradication
Helicobacter pylori
Microbiota
Tongue coating
Journal
BMC microbiology
ISSN: 1471-2180
Titre abrégé: BMC Microbiol
Pays: England
ID NLM: 100966981
Informations de publication
Date de publication:
18 Oct 2024
18 Oct 2024
Historique:
received:
02
01
2024
accepted:
14
10
2024
medline:
19
10
2024
pubmed:
19
10
2024
entrez:
18
10
2024
Statut:
epublish
Résumé
Eradicating Helicobacter pylori (H. pylori) can cause an imbalance in the microbiota. Dysbiosis of the gut microbiome may produce multiple diseases and bacterial infections. The objective of this study was to investigate the influence of Helicobacter pylori (H. pylori) infection and its eradication on the composition of the oral tongue coating microbiota. A cohort of 35 participants was recruited and categorized into two groups: the H. pylori negative group (N group) consisting of 12 individuals and the H. pylori positive group (23 individuals). Within the H. pylori positive group, subjects were further stratified into the H. pylori pre-eradicated group (HPQ group) and the H. pylori eradicated group (HPH group). H. pylori positive individuals were treated with a quadruple regimen containing bismuth, and tongue coating samples were collected both prior to and following treatment. Concurrently, tongue coating samples were collected from H. pylori negative individuals. High-throughput 16S rRNA sequencing technology was employed to assess the microbial composition of the tongue coating in the N group, HPQ group, and HPH group. Pertinent clinical data were documented.Microbial diversity was found to significantly differ among the N group, HPQ group, and HPH group, as evidenced by variations in Chao1 index, Shannon index, and Partial Least Squares Discriminant Analysis (PLS-DA). The dominant bacterial phyla identified across all groups included Bacteroidetes, Proteobacteria, Firmicutes, Fusobacteria, Actinobacteria, and Saccharibacteria. At the phylum level, Firmicutes exhibited higher relative abundance in the HPQ group in comparison to both the N group and HPH group. Conversely, Bacteroidetes displayed greater prevalence in the N group and HPH group. Linear Discriminant Analysis Effect Size (LEfSe) analysis indicated a higher abundance of Romboutsia, Rothia, and Turiciactor in the HPQ group. Our study revealed significant disparities in microbial diversity and richness among the three groups. Furthermore, our findings suggest a potential association between the presence of Streptococcus, Rothia and H. pylori positive individuals.
Identifiants
pubmed: 39425053
doi: 10.1186/s12866-024-03584-y
pii: 10.1186/s12866-024-03584-y
doi:
Substances chimiques
RNA, Ribosomal, 16S
0
Anti-Bacterial Agents
0
DNA, Bacterial
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
416Informations de copyright
© 2024. The Author(s).
Références
Marshall BJ, Windsor HM. The relation of Helicobacter pylori to gastric adenocarcinoma and lymphoma: pathophysiology, epidemiology, screening, clinical presentation, treatment, and prevention. Med Clin North Am. 2005;89(2):313–44. viii.
doi: 10.1016/j.mcna.2004.09.001
pubmed: 15656929
Klymiuk I, Bilgilier C, Stadlmann A, Thannesberger J, Kastner MT, Högenauer C, et al. The human gastric microbiome is predicated upon infection with helicobacter pylori. Front Microbiol. 2017;8:2508.
doi: 10.3389/fmicb.2017.02508
pubmed: 29312210
pmcid: 5735373
Iino C, Shimoyama T, Chinda D, Sakuraba H, Fukuda S, Nakaji S. Influence of helicobacter pylori infection and atrophic gastritis on the gut microbiota in a Japanese population. Digestion. 2020;101(4):422–32.
doi: 10.1159/000500634
pubmed: 31394526
Chen T, Yu WH, Izard J, Baranova OV, Lakshmanan A, Dewhirst FE. The Human Oral Microbiome Database: a web accessible resource for investigating oral microbe taxonomic and genomic information. Database (Oxford). 2010;2010:baq013.
doi: 10.1093/database/baq013
pubmed: 20624719
Chua EG, Chong JY, Lamichhane B, Webberley KM, Marshall BJ, Wise MJ, et al. Gastric Helicobacter pylori infection perturbs human oral microbiota. PeerJ. 2019;7:e6336.
doi: 10.7717/peerj.6336
pubmed: 30713820
pmcid: 6354663
He C, Peng C, Wang H, Ouyang Y, Zhu Z, Shu X, et al. The eradication of Helicobacter pylori restores rather than disturbs the gastrointestinal microbiota in asymptomatic young adults. Helicobacter. 2019;24(4):e12590.
doi: 10.1111/hel.12590
pubmed: 31124220
Jakobsson HE, Jernberg C, Andersson AF, Sjölund-Karlsson M, Jansson JK, Engstrand L. Short-term antibiotic treatment has differing long-term impacts on the human throat and gut microbiome. PLoS One. 2010;5(3):e9836.
doi: 10.1371/journal.pone.0009836
pubmed: 20352091
pmcid: 2844414
Kageyama S, Takeshita T, Furuta M, Tomioka M, Asakawa M, Suma S, et al. Relationships of variations in the tongue microbiota and pneumonia mortality in nursing home residents. J Gerontol A Biol Sci Med Sci. 2018;73(8):1097–102.
doi: 10.1093/gerona/glx205
pubmed: 29053769
Wu J, Xu S, Xiang C, Cao Q, Li Q, Huang J, et al. Tongue coating microbiota community and risk effect on gastric cancer. J Cancer. 2018;9(21):4039–48.
doi: 10.7150/jca.25280
pubmed: 30410609
pmcid: 6218773
Lu H, Ren Z, Li A, Li J, Xu S, Zhang H, et al. Tongue coating microbiome data distinguish patients with pancreatic head cancer from healthy controls. J Oral Microbiol. 2019;11(1):1563409.
doi: 10.1080/20002297.2018.1563409
pubmed: 30728915
pmcid: 6352935
Gotoda T, Takano C, Kusano C, Suzuki S, Ikehara H, Hayakawa S, et al. Gut microbiome can be restored without adverse events after Helicobacter pylori eradication therapy in teenagers. Helicobacter. 2018;23(6):e12541.
doi: 10.1111/hel.12541
pubmed: 30311721
Liou JM, Chen CC, Chang CM, Fang YJ, Bair MJ, Chen PY, et al. Taiwan gastrointestinal disease and helicobacter consortium. Long-term changes of gut microbiota, antibiotic resistance, and metabolic parameters after Helicobacter pylori eradication: a multicentre, open-label, randomised trial. Lancet Infect Dis. 2019;19(10):1109–20.
doi: 10.1016/S1473-3099(19)30272-5
pubmed: 31559966
Desai HG, Gill HH, Shankaran K, Mehta PR, Prabhu SR. Dental plaque: a permanent reservoir of Helicobacter pylori? Scand J Gastroenterol. 1991;26(11):1205–8.
doi: 10.3109/00365529108998615
pubmed: 1754858
Hu Y, Xu X, Ouyang YB, He C, Li NS, Xie C, Peng C, Zhu ZH, Xie Y, Shu X, Lu NH, Zhu Y. Analysis of oral microbiota alterations induced by Helicobacter pylori infection and vonoprazan-amoxicillin dual therapy for Helicobacter pylori eradication. Helicobacter. 2022;27(5):e12923.
doi: 10.1111/hel.12923
pubmed: 36036087
Ji Y, Liang X, Lu H. Analysis of by high-throughput sequencing: helicobacter pylori infection and salivary microbiome. BMC Oral Health. 2020;20(1):84.
doi: 10.1186/s12903-020-01070-1
pubmed: 32197614
pmcid: 7333272
Zou Y, Ju X, Chen W, Yuan J, Wang Z, Aluko RE, et al. Rice bran attenuated obesity via alleviating dyslipidemia, browning of white adipocytes and modulating gut microbiota in high-fat diet-induced obese mice. Food Funct. 2020;11(3):2406–17.
doi: 10.1039/C9FO01524H
pubmed: 32129359
Koliada A, Syzenko G, Moseiko V, Budovska L, Puchkov K, Perederiy V, et al. Association between body mass index and Firmicutes/Bacteroidetes ratio in an adult Ukrainian population. BMC Microbiol. 2017;17(1):120.
doi: 10.1186/s12866-017-1027-1
pubmed: 28532414
pmcid: 5440985
Krajmalnik-Brown R, Ilhan ZE, Kang DW, DiBaise JK, et al. Effects of gut microbes on nutrient absorption and energy regulation. Nutr Clin Pract. 2012;27(2):201–14.
doi: 10.1177/0884533611436116
pubmed: 22367888
pmcid: 3601187
Bloch S, Hager-Mair FF, Andrukhov O, Schäffer C. Oral streptococci: modulators of health and disease. Front Cell Infect Microbiol. 2024;14:1357631.
doi: 10.3389/fcimb.2024.1357631
pubmed: 38456080
pmcid: 10917908
Senthil Kumar S, Johnson MDL, Wilson JE. Insights into the enigma of oral streptococci in carcinogenesis. Microbiol Mol Biol Rev. 2024;88(2):e0009523.
doi: 10.1128/mmbr.00095-23
pubmed: 38506551
Tarrah A, de Castilhos J, Rossi RC, Duarte VDS, Ziegler DR, Corich V, Giacomini A. In vitro probiotic potential and anti-cancer activity of newly isolated folate-producing streptococcus thermophilus strains. Front Microbiol. 2018;9:2214.
doi: 10.3389/fmicb.2018.02214
pubmed: 30283428
pmcid: 6156529
Patterson KG, Dixon Pittaro JL, Bastedo PS, Hess DA, Haeryfar SM, McCormick JK. Control of established colon cancer xenografts using a novel humanized single chain antibody-streptococcal superantigen fusion protein targeting the 5T4 oncofetal antigen. PLoS One. 2014;9(4):e95200.
doi: 10.1371/journal.pone.0095200
pubmed: 24736661
pmcid: 3988171
Elkattawy S, Alyacoub R, Younes I, Mowafy A, Noori M, Mirza M. A rare report of Rothia dentocariosa endocarditis. J Community Hosp Intern Med Perspect. 2021;11(3):413–5.
doi: 10.1080/20009666.2021.1880539
pubmed: 34234918
pmcid: 8118413
Fatahi-Bafghi M. Characterization of the Rothia spp. and their role in human clinical infections. Infect Genet Evol. 2021;93:104877.
doi: 10.1016/j.meegid.2021.104877
pubmed: 33905886
Kohi S, Macgregor-Das A, Dbouk M, Yoshida T, Chuidian M, Abe T, Borges M, Lennon AM, Shin EJ, Canto MI, Goggins M. Alterations in the duodenal fluid microbiome of patients with pancreatic cancer. Clin Gastroenterol Hepatol. 2022;20(2):e196–227.
doi: 10.1016/j.cgh.2020.11.006
pubmed: 33161160
Elghannam MT, Hassanien MH, Ameen YA, Turky EA, ELattar GM, ELRay AA, ELTalkawy MD. Helicobacter pylori and oral-gut microbiome: clinical implications. Infection. 2024;52(2):289–300.
doi: 10.1007/s15010-023-02115-7
pubmed: 37917397
Fu K, Cheung AHK, Wong CC, Liu W, Zhou Y, Wang F, Huang P, Yuan K, Coker OO, Pan Y, Chen D, Lam NM, Gao M, Zhang X, Huang H, To KF, Sung JJY, Yu J. Streptococcus anginosus promotes gastric inflammation, atrophy, and tumorigenesis in mice. Cell. 2024;187(4):882–896.e17.
doi: 10.1016/j.cell.2024.01.004
pubmed: 38295787