Novel Vibrational Proteins.

Vibration Spectrum Analysis, Raman Green Fluorescent Proteins / chemistry Alkynes / chemistry Protein Engineering

Journal

Analytical chemistry
ISSN: 1520-6882
Titre abrégé: Anal Chem
Pays: United States
ID NLM: 0370536

Informations de publication

Date de publication:
22 Oct 2024
Historique:
medline: 22 10 2024
pubmed: 22 10 2024
entrez: 22 10 2024
Statut: ppublish

Résumé

Genetically encoded green fluorescent protein (GFP) and its brighter and redder variants have tremendously revolutionized modern molecular biology and life science by enabling direct visualization of gene regulated protein functions on microscopic and nanoscopic scales. However, the current fluorescent proteins (FPs) only emit a few colors with an emission width of about 30-50 nm. Here, we engineer novel vibrational proteins (VPs) that undergo much finer vibrational transitions and emit rather narrow vibrational spectra (0.1-0.3 nm, roughly 3-10 cm

Identifiants

DOI: 10.1021/acs.analchem.4c01569 PMID: 39434664
pubmed: 39434664
doi: 10.1021/acs.analchem.4c01569
doi:

Substances chimiques

Green Fluorescent Proteins 147336-22-9
Alkynes 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

16481-16486

Auteurs

Yage Chen (Y)

Britton Chance Center and MoE Key Laboratory for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.
ORCID: 0000-0002-6601-5198

Zhiliang Huang (Z)

Britton Chance Center and MoE Key Laboratory for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.
Changping Laboratory, Beijing 102206, China.

Erli Cai (E)

Britton Chance Center and MoE Key Laboratory for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.

Shuchen Zhong (S)

Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, PKU-Tsinghua Center for Life Science, Center for Quantitate Biology, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.

Haozheng Li (H)

Changping Laboratory, Beijing 102206, China.

Wei Ju (W)

Britton Chance Center and MoE Key Laboratory for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.
Changping Laboratory, Beijing 102206, China.

Jie Yang (J)

Britton Chance Center and MoE Key Laboratory for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.

Wei Chen (W)

Britton Chance Center and MoE Key Laboratory for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.
ORCID: 0000-0001-5567-7463

Chun Tang (C)

Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, PKU-Tsinghua Center for Life Science, Center for Quantitate Biology, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.
ORCID: 0000-0001-6477-6500

Ping Wang (P)

Britton Chance Center and MoE Key Laboratory for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.
Changping Laboratory, Beijing 102206, China.
Huaiyin Institute of Technology, Huaian, Jiangsu 223003, China.
ORCID: 0000-0002-5168-6829

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Classifications MeSH

questionsmedicales.fr › Phénomènes physiques › Phénomènes mécaniques › Vibration › Novel Vibrational Proteins.
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Analyse spectrale › Analyse spectrale Raman › Novel Vibrational Proteins.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines luminescentes › Protéines à fluorescence verte › Novel Vibrational Proteins.
questionsmedicales.fr › Composés chimiques organiques › Hydrocarbures › Hydrocarbures acycliques › Alcynes › Novel Vibrational Proteins.
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Génie génétique › Ingénierie des protéines › Novel Vibrational Proteins.