Plasma SQSTM1/p62 act as a biomarker for steroid-induced osteonecrosis of the femoral head.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
22 Oct 2024
Historique:
received: 14 04 2024
accepted: 30 08 2024
medline: 23 10 2024
pubmed: 23 10 2024
entrez: 22 10 2024
Statut: epublish

Résumé

Autophagy is closely associated with the onset and progression of steroid-induced osteonecrosis of the femoral head (SIONFH). SQSTM1/p62 is an important indicator of autophagic activity. The aim of this study was to investigate the role of SQSTM1/p62 in the development of SIONFH. From May 2021 through November 2021, 36 patients diagnosed with SIONFH and 36 healthy controls were recruited for this study. Evaluations included imaging and pathologic assessment of clinical bone tissue, location and level of SQSTM1/p62 expression, plasma SQSTM1/p62 levels, and receiver operating characteristic (ROC) curves. We observed that the expression level of SQSTM1/p62 in bone samples decreased with the Association Research Circulation Osseous (ARCO) phase. Plasma SQSTM1/p62 levels were significantly higher in the SIONFH group compared to healthy controls. Plasma SQSTM1/p62 levels were higher in pre-crash patients than in post-crash patients, and lower plasma SQSTM1/p62 levels were associated with elevated ARCO stage. Plasma SQSTM1/p62 may represent a potential biomarker for different stages during SIONFH. Lower plasma SQSTM1/p62 levels indicate an advanced stage of SIONFH. This study provides new clues for early diagnosis of SIONFH.

Identifiants

pubmed: 39438530
doi: 10.1038/s41598-024-71743-2
pii: 10.1038/s41598-024-71743-2
doi:

Substances chimiques

Sequestosome-1 Protein 0
SQSTM1 protein, human 0
Biomarkers 0
Steroids 0
P62 protein, human 0
RNA-Binding Proteins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

24932

Subventions

Organisme : National Natural Science Foundation of China
ID : 82004392
Organisme : National Natural Science Foundation of China
ID : 82274544
Organisme : National Natural Science Foundation of China
ID : 81573996
Organisme : the major project of "Double First-class" and High-level University Discipline Collaborative Innovation Team of Guangzhou University of Chinese Medicine
ID : 2021XK05
Organisme : the cultivated project of "Double First-class" and High-level University Discipline Collaborative Innovation Team of Guangzhou University of Chinese Medicine
ID : 2021XK41
Organisme : the cultivated project of "Double First-class" and High-level University Discipline Collaborative Innovation Team of Guangzhou University of Chinese Medicine
ID : 2021XK46
Organisme : Bijie Science and Technology Bureau 'the open competition project'
ID : no. BST Major Project No.1,2022

Informations de copyright

© 2024. The Author(s).

Références

Xu, H., Zhao, J. & Shi, C. Current status of clinical research on the pathogenesis and treatment of femoral head necrosis. Panminerva Med. https://doi.org/10.23736/S0031-0808.23.04929-7 (2023).
doi: 10.23736/S0031-0808.23.04929-7 pubmed: 38093627
Petek, D., Hannouche, D. & Suva, D. Osteonecrosis of the femoral head: Pathophysiology and current concepts of treatment. EFORT Open Rev. 4(3), 85–97 (2019).
doi: 10.1302/2058-5241.4.180036 pubmed: 30993010 pmcid: 6440301
Bergman, J., Nordström, A. & Nordström, P. Epidemiology of osteonecrosis among older adults in Sweden. Osteoporos. Int. 30(5), 965–973 (2019).
doi: 10.1007/s00198-018-04826-2 pubmed: 30627759 pmcid: 6502772
Dima, A., Pedersen, A. B., Pedersen, L., Baicus, C. & Thomsen, R. W. Association of common comorbidities with osteonecrosis: A nationwide population-based case-control study in Denmark. BMJ Open 8(2), e020680 (2018).
doi: 10.1136/bmjopen-2017-020680 pubmed: 29439082 pmcid: 5829903
Fukushima, W. et al. Nationwide epidemiologic survey of idiopathic osteonecrosis of the femoral head. Clin. Orthop. Relat. Res. 468(10), 2715–2724 (2010).
doi: 10.1007/s11999-010-1292-x pubmed: 20224959 pmcid: 2939331
Zhao, D. et al. Guidelines for clinical diagnosis and treatment of osteonecrosis of the femoral head in adults (2019 version). J. Orthop. Transl. 21, 100–110 (2020).
Kubo, T. et al. Clinical and basic research on steroid-induced osteonecrosis of the femoral head in Japan. J. Orthop. Sci. 21(4), 407–413 (2016).
doi: 10.1016/j.jos.2016.03.008 pubmed: 27062553
Luo, P., Gao, F., Han, J., Sun, W. & Li, Z. The role of autophagy in steroid necrosis of the femoral head: A comprehensive research review. Int. Orthop. 42(7), 1747–1753 (2018).
doi: 10.1007/s00264-018-3994-8 pubmed: 29797168
Liu, B. et al. Denosumab can prevent collapse in patients with early-stage steroid-induced osteonecrosis of the femoral head by inhibiting osteoclasts and autophagy. Orthop. Surg. 15(1), 256–265 (2023).
doi: 10.1111/os.13584 pubmed: 36398455
Liu, J. et al. Selective autophagy in cancer: Mechanisms, therapeutic implications, and future perspectives. Mol. Cancer 23(1), 22 (2024).
doi: 10.1186/s12943-024-01934-y pubmed: 38262996 pmcid: 10807193
Debnath, J., Gammoh, N. & Ryan, K. M. Autophagy and autophagy-related pathways in cancer. Nat. Rev. Mol. Cell Biol. 24(8), 560–575 (2023).
doi: 10.1038/s41580-023-00585-z pubmed: 36864290
Allen, E. A. & Baehrecke, E. H. Autophagy in animal development. Cell Death Differ. 27(3), 903–918 (2020).
doi: 10.1038/s41418-020-0497-0 pubmed: 31988494 pmcid: 7206001
Li, X. et al. Dual target PARP1/EZH2 inhibitors inducing excessive autophagy and producing synthetic lethality for triple-negative breast cancer therapy. Eur. J. Med. Chem. 265, 116054 (2024).
doi: 10.1016/j.ejmech.2023.116054 pubmed: 38134746
Miyamoto, S. Autophagy and cardiac aging. Cell Death Differ. 26(4), 653–664 (2019).
doi: 10.1038/s41418-019-0286-9 pubmed: 30692640 pmcid: 6460392
Ichimiya, T. et al. Autophagy and autophagy-related diseases: A review. Int. J. Mol. Sci. 21, 23 (2020).
doi: 10.3390/ijms21238974
Kirkin, V. & Rogov, V. V. A diversity of selective autophagy receptors determines the specificity of the autophagy pathway. Mol. Cell 76(2), 268–285 (2019).
doi: 10.1016/j.molcel.2019.09.005 pubmed: 31585693
Fan, X. et al. p62 works as a hub modulation in the ageing process. Age. Res. Rev. 73, 101538 (2022).
doi: 10.1016/j.arr.2021.101538
Tan, C. T., Soh, N. J. H., Chang, H. C. & Yu, V. C. p62/SQSTM1 in liver diseases: The usual suspect with multifarious identities. FEBS J. 290(4), 892–912 (2023).
doi: 10.1111/febs.16317 pubmed: 34882306
Wang, B. J., Tang, Y. D., Yu, B. Y., Gui, D. & Xu, H. Expression of autophagy-related factor p62 for lung cancer diagnosis and prognosis: A systematic review and meta-analysis. Math. Biosci. Eng. 16(6), 6805–6821 (2019).
doi: 10.3934/mbe.2019340 pubmed: 31698589
Yoon, B. H. et al. Etiologic classification criteria of ARCO on femoral head osteonecrosis part 1: Glucocorticoid-associated osteonecrosis. J. Arthroplasty 34(1), 163–168 (2019).
doi: 10.1016/j.arth.2018.09.005 pubmed: 30348552
Huang, Z., Wang, Q., Zhang, T., Fu, Y. & Wang, W. Hyper-activated platelet lysates prevent glucocorticoid-associated femoral head necrosis by regulating autophagy. Biomed. Pharmacother. 139, 111711 (2021).
doi: 10.1016/j.biopha.2021.111711 pubmed: 34243617
Sbardella, D., Tundo, G. R., Coletta, M., Manni, G. & Oddone, F. Dexamethasone downregulates autophagy through accelerated turn-over of the Ulk-1 complex in a trabecular meshwork cells strain: Insights on steroid-induced glaucoma pathogenesis. Int. J. Mol. Sci. 22, 11 (2021).
doi: 10.3390/ijms22115891
Kriel, J. & Loos, B. The good, the bad and the autophagosome: Exploring unanswered questions of autophagy-dependent cell death. Cell Death Differ. 26(4), 640–652 (2019).
doi: 10.1038/s41418-018-0267-4 pubmed: 30659234 pmcid: 6460391
Yun, H. R. et al. Roles of autophagy in oxidative stress. Int. J. Mol. Sci. 21, 9 (2020).
doi: 10.3390/ijms21093289
Liu, S., Yao, S., Yang, H., Liu, S. & Wang, Y. Autophagy: Regulator of cell death. Cell Death Dis. 14(10), 648 (2023).
doi: 10.1038/s41419-023-06154-8 pubmed: 37794028 pmcid: 10551038
Jia, J. et al. Glucocorticoid dose determines osteocyte cell fate. FASEB J. 25(10), 3366–3376 (2011).
doi: 10.1096/fj.11-182519 pubmed: 21705669 pmcid: 3177583
Lai, E. H. et al. Simvastatin alleviates the progression of periapical lesions by modulating autophagy and apoptosis in osteoblasts. J. Endod. 38(6), 757–763 (2012).
doi: 10.1016/j.joen.2012.02.023 pubmed: 22595108
Wang, L., Heckmann, B. L., Yang, X. & Long, H. Osteoblast autophagy in glucocorticoid-induced osteoporosis. J. Cell. Physiol. 234(4), 3207–3215 (2019).
doi: 10.1002/jcp.27335 pubmed: 30417506
Liu, W. J. et al. p62 links the autophagy pathway and the ubiqutin-proteasome system upon ubiquitinated protein degradation. Cell. Mol. Biol. Lett. 21, 29 (2016).
doi: 10.1186/s11658-016-0031-z pubmed: 28536631 pmcid: 5415757
Jeong, S. J., Zhang, X., Rodriguez-Velez, A., Evans, T. D. & Razani, B. p62/SQSTM1 and selective autophagy in cardiometabolic diseases. Antioxid. Redox Signal. 31(6), 458–471 (2019).
doi: 10.1089/ars.2018.7649 pubmed: 30588824 pmcid: 6653798
Liu, W. et al. Dexamethasone-induced production of reactive oxygen species promotes apoptosis via endoplasmic reticulum stress and autophagy in MC3T3-E1 cells. Int. J. Mol. Med. 41(4), 2028–2036 (2018).
pubmed: 29393368 pmcid: 5810234
He, M. C. et al. Osteoclastic activity was associated with the development of steroid-induced osteonecrosis of femoral head. Artif. Cells Nanomed. Biotechnol. 48(1), 1036–1046 (2020).
doi: 10.1080/21691401.2020.1774596 pubmed: 32667225
Ma, J. et al. Extracellular vesicles from BMSCs prevent glucocorticoid-induced BMECs injury by regulating autophagy via the PI3K/Akt/mTOR pathway. Cells 11, 13 (2022).
doi: 10.3390/cells11132104
Peng, P., Nie, Z., Sun, F. & Peng, H. Glucocorticoids induce femoral head necrosis in rats through the ROS/JNK/c-Jun pathway. FEBS Open Bio 11(1), 312–321 (2021).
doi: 10.1002/2211-5463.13037 pubmed: 33190410

Auteurs

Wenyuan Hou (W)

Guangzhou University of Chinese Medicine, Guangzhou, China.

Peng Peng (P)

Guangzhou University of Chinese Medicine, Guangzhou, China.

Fangjun Xiao (F)

Guangzhou University of Chinese Medicine, Guangzhou, China.

Jiaqing Tian (J)

Guangzhou University of Chinese Medicine, Guangzhou, China.

Xianshun He (X)

Guangzhou University of Chinese Medicine, Guangzhou, China.

Shun Lu (S)

Guangzhou University of Chinese Medicine, Guangzhou, China.

Huan Xiao (H)

Department of Orthopedics, Bijie Traditional Chinese Medicine Hospital, Bijie, China.

Mincong He (M)

The Third Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.
Guangdong Research Institute for Orthopedics and Traumatology of Chinese Medicine, Guangzhou, China.

Qiushi Wei (Q)

The Third Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China. weiqshi@126.com.
Guangdong Research Institute for Orthopedics and Traumatology of Chinese Medicine, Guangzhou, China. weiqshi@126.com.
State Key Laboratory of Traditional Chinese Medicine Syndrome, Guangzhou University of Chinese Medicine, Guangzhou, China. weiqshi@126.com.

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