GHRH-stimulated pituitary small extracellular vesicles inhibit hepatocyte proliferation and IGF-1 expression by its cargo miR-375-3p.
GHRH
Hepatocyte
IGF-1
Pituitary small extracellular vesicles
miR-375-3p
Journal
Journal of nanobiotechnology
ISSN: 1477-3155
Titre abrégé: J Nanobiotechnology
Pays: England
ID NLM: 101152208
Informations de publication
Date de publication:
22 Oct 2024
22 Oct 2024
Historique:
received:
18
03
2024
accepted:
14
09
2024
medline:
23
10
2024
pubmed:
23
10
2024
entrez:
22
10
2024
Statut:
epublish
Résumé
Small extracellular vesicles (sEV) have emerged as a novel mode of intercellular material transport and information transmission. It has been suggested hormones may regulate the production and function of sEV. However, the specific impact of growth hormone-releasing hormone (GHRH) on pituitary sEV production and the role of sEV in the regulation of the GHRH-GH-IGF axis has not been previously reported. The results of the present study demonstrated that GHRH increased the production of pituitary sEV by promoting the expression of Rab27a. More importantly, GHRH induced alterations in protein and miRNA levels within GH3-sEV components. Notably, GH3-sEV with GHRH treatment exhibited the enhanced ability to impede BRL 3A cell proliferation and the expression of IGF-1. Conclusively, for the first time, we corroborate the influence of GHRH on pituitary sEV, thereby presenting novel evidence for how sEV participates in the balance of the GHRH-GH-IGF axis. Importantly, this study provides new insight into a novel balance mechanism mediated by sEV within the endocrine system.
Identifiants
pubmed: 39438882
doi: 10.1186/s12951-024-02857-y
pii: 10.1186/s12951-024-02857-y
doi:
Substances chimiques
Growth Hormone-Releasing Hormone
9034-39-3
Insulin-Like Growth Factor I
67763-96-6
MicroRNAs
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
649Subventions
Organisme : National Natural Science Foundation of China
ID : 31802156
Organisme : National Natural Science Foundation of China
ID : 32072814
Organisme : National Natural Science Foundation of China
ID : 32072812
Organisme : Natural Science Foundation of Guangzhou Province
ID : 2021A1515011310
Organisme : Natural Science Foundation of Guangzhou Province
ID : 2020A1515010062
Informations de copyright
© 2024. The Author(s).
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