Characterization of Medusavirus encoded histones reveals nucleosome-like structures and a unique linker histone.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
23 Oct 2024
23 Oct 2024
Historique:
received:
13
04
2024
accepted:
08
10
2024
medline:
24
10
2024
pubmed:
24
10
2024
entrez:
23
10
2024
Statut:
epublish
Résumé
The organization of DNA into nucleosomes is a ubiquitous and ancestral feature that was once thought to be exclusive to the eukaryotic domain of life. Intriguingly, several representatives of the Nucleocytoplasmic Large DNA Viruses (NCLDV) encode histone-like proteins that in Melbournevirus were shown to form nucleosome-like particles. Medusavirus medusae (MM), a distantly related giant virus, encodes all four core histone proteins and, unique amongst most giant viruses, a putative acidic protein with two domains resembling eukaryotic linker histone H1. Here, we report the structure of nucleosomes assembled with MM histones and highlight similarities and differences with eukaryotic and Melbournevirus nucleosomes. Our structure provides insight into how variations in histone tail and loop lengths are accommodated within the context of the nucleosome. We show that MM-histones assemble into tri-nucleosome arrays, and that the putative linker histone H1 does not function in chromatin compaction. These findings expand our limited understanding of chromatin organization by virus-encoded histones.
Identifiants
pubmed: 39443461
doi: 10.1038/s41467-024-53364-5
pii: 10.1038/s41467-024-53364-5
doi:
Substances chimiques
Histones
0
Nucleosomes
0
Viral Proteins
0
Chromatin
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
9138Subventions
Organisme : Howard Hughes Medical Institute (HHMI)
ID : NA
Organisme : NIGMS NIH HHS
ID : T32 GM142607
Pays : United States
Organisme : Damon Runyon Cancer Research Foundation (Cancer Research Fund of the Damon Runyon-Walter Winchell Foundation)
ID : DRG-2499-23
Organisme : Damon Runyon Cancer Research Foundation (Cancer Research Fund of the Damon Runyon-Walter Winchell Foundation)
ID : DRG-2499-23
Informations de copyright
© 2024. The Author(s).
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