Establishment of recombinase polymerase amplification detection method for Dactylobotrys graminicola.
Dactylobotrys graminicola
Hulless barley sheath rot disease
Recombinase polymerase amplification lateral flow dipstick (RPA-LFD)
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
23 10 2024
23 10 2024
Historique:
received:
31
05
2024
accepted:
17
10
2024
medline:
24
10
2024
pubmed:
24
10
2024
entrez:
24
10
2024
Statut:
epublish
Résumé
Hulless barley sheath rot is a spike disease caused by Dactylobotrys graminicola. In recent years, it has generally occurred in hulless barley growing areas in China, resulting in reduced hulless barley yields. In this study, primers and probes were designed based on conserved genome sequences, and a method was established using recombinant enzyme polymerase amplification-lateral flow burette (RPA-LFD) technology for the rapid diagnosis of sheath rot in hulless barley. The method can be successfully established in five minutes at a constant temperature of 39℃, and the results are consistent with those of normal PCR analysis. The method demonstrated high sensitivity, with a detection limit of 10 fg/µL. Furthermore, the rapid method was able to successfully detect D. graminicola in hulless barley during field incubation, which highlighted the significant advantage of the method in practical applications. In conclusion, the RPA method established in this study exhibited several advantageous characteristics, including high efficiency, simplicity, rapidity and practicality, which provide a theoretical basis for the early detection and prevention of D. graminicola.
Identifiants
pubmed: 39443612
doi: 10.1038/s41598-024-76921-w
pii: 10.1038/s41598-024-76921-w
doi:
Substances chimiques
Recombinases
0
DNA Primers
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
25079Subventions
Organisme : basic research project of Qinghai, China
ID : 2023-ZJ-944J
Organisme : basic research project of Qinghai, China
ID : 2023-ZJ-944J
Organisme : basic research project of Qinghai, China
ID : 2023-ZJ-944J
Organisme : basic research project of Qinghai, China
ID : 2023-ZJ-944J
Organisme : basic research project of Qinghai, China
ID : 2023-ZJ-944J
Informations de copyright
© 2024. The Author(s).
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