Noninvasive in vivo photoacoustic detection of malaria with Cytophone in Cameroon.

Humans Cameroon Photoacoustic Techniques / methods Adult Erythrocytes / parasitology Cross-Sectional Studies Flow Cytometry / methods Female Malaria / diagnosis Male Hemeproteins / analysis Malaria, Falciparum / diagnosis Sensitivity and Specificity Longitudinal Studies Young Adult Parasitemia / diagnosis Plasmodium falciparum / isolation & purification Middle Aged ROC Curve Adolescent

Journal

Nature communications

ISSN: 2041-1723

Titre abrégé: Nat Commun

Pays: England

ID NLM: 101528555

Informations de publication

Date de publication:
25 Oct 2024

Historique:

received: 16 04 2024

accepted: 07 10 2024

medline: 26 10 2024

pubmed: 26 10 2024

entrez: 25 10 2024

Statut: epublish

Résumé

Current malaria diagnostics are invasive, lack sensitivity, and rapid tests are plagued by deletions in target antigens. Here we introduce the Cytophone, an innovative photoacoustic flow cytometer platform with high-pulse-rate lasers and a focused ultrasound transducer array to noninvasively detect and identify malaria-infected red blood cells (iRBCs) using specific wave shapes, widths, and time delays generated from the absorbance of laser energy by hemozoin, a universal biomarker of malaria infection. In a population of Cameroonian adults with uncomplicated malaria, we assess our device for safety in a cross-sectional cohort (n = 10) and conduct a performance assessment in a longitudinal cohort (n = 20) followed for 30 ± 7 days after clearance of parasitemia. Longitudinal cytophone measurements are compared to point-of-care and molecular assays (n = 94). Cytophone is safe with 90% sensitivity, 69% specificity, and a receiver-operator-curve-area-under-the-curve (ROC-AUC) of 0.84, as compared to microscopy. ROC-AUCs of Cytophone, microscopy, and RDT compared to quantitative PCR are not statistically different from one another. The ability to noninvasively detect iRBCs in the bloodstream is a major advancement which offers the potential to rapidly identify both the large asymptomatic reservoir of infection, as well as diagnose symptomatic cases without the need for a blood sample.

Identifiants

DOI: 10.1038/s41467-024-53243-z PMID: 39455558

pubmed: 39455558

doi: 10.1038/s41467-024-53243-z

pii: 10.1038/s41467-024-53243-z

doi:

Substances chimiques

Hemeproteins 0

hemozoin 39404-00-7

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

Pagination

9228

Subventions

Organisme : Division of Intramural Research, National Institute of Allergy and Infectious Diseases (Division of Intramural Research of the NIAID)

ID : R21AI160171

Organisme : U.S. Department of Health & Human Services | NIH | Fogarty International Center (FIC)

ID : D43TW010540

Informations de copyright

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