Mixed and membrane-separated culturing of synthetic cyanobacteria-yeast consortia reveals metabolic cross-talk mimicking natural cyanolichens.
Cross-feeding
Membrane-separated bioreactor
Metabolite exchange
Microbial community
Phototroph-heterotroph co-culture
Synthetic lichen
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
25 Oct 2024
25 Oct 2024
Historique:
received:
20
07
2024
accepted:
30
09
2024
medline:
26
10
2024
pubmed:
26
10
2024
entrez:
25
10
2024
Statut:
epublish
Résumé
Metabolite exchange mediates crucial interactions in microbial communities, significantly impacting global carbon and nitrogen cycling. Understanding these chemically-mediated interactions is essential for elucidating natural community functions and developing engineered synthetic communities. This study investigated membrane-separated bioreactors (mBRs) as a novel tool to identify transient metabolites and their producers/consumers in mixed microbial communities. We compared three co-culture methods (direct mixed, 2-chamber mBR, and 3-chamber mBR) to grow a synthetic binary community of the cyanobacterium Synechococcus elongatus PCC 7942 and the fungus Rhodotorula toruloides NBRC 0880, as well as axenic S. elongatus. Despite not being natural lichen constituents, these organisms exhibited interactions resembling those in cyanolichens. S. elongatus fixed CO
Identifiants
pubmed: 39455633
doi: 10.1038/s41598-024-74743-4
pii: 10.1038/s41598-024-74743-4
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
25303Subventions
Organisme : Laboratory Directed Research and Development Program at Pacific Northwest National Laboratory
ID : Predictive Phenomics Initiative
Organisme : Laboratory Directed Research and Development Program at Pacific Northwest National Laboratory
ID : Predictive Phenomics Initiative
Organisme : Laboratory Directed Research and Development Program at Pacific Northwest National Laboratory
ID : Predictive Phenomics Initiative
Organisme : Laboratory Directed Research and Development Program at Pacific Northwest National Laboratory
ID : Predictive Phenomics Initiative
Organisme : Laboratory Directed Research and Development Program at Pacific Northwest National Laboratory
ID : Predictive Phenomics Initiative
Organisme : U.S. Department of Energy Genomic Science Program
ID : DE-SC0019388
Organisme : U.S. Department of Energy Genomic Science Program
ID : DE-SC0019388
Organisme : U.S. Department of Energy Genomic Science Program
ID : DE-SC0019388
Organisme : U.S. Department of Energy Genomic Science Program
ID : DE-SC0019388
Informations de copyright
© 2024. Battelle Memorial Institute and The Authors 2024.
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