Spatiotemporal control of transgene expression using an infrared laser in the crustacean Daphnia magna.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
28 Oct 2024
28 Oct 2024
Historique:
received:
12
07
2024
accepted:
22
10
2024
medline:
28
10
2024
pubmed:
28
10
2024
entrez:
28
10
2024
Statut:
epublish
Résumé
The crustacean Daphnia magna is an emerging model for ecological and toxicological genomics. However, the lack of methods for spatial and temporal control of gene expression has impaired the elucidation of molecular mechanisms underlying responses to environments in vivo. Here we report local activation of the hsp70 promoter-driven gene cassette in D. magna by the infrared laser-evoked gene operator (IR-LEGO), a method for heating the target cells with infrared irradiation. We identified the heat-inducible promoter upstream of the D. magna hsp70-A gene. Using this promoter, we generated a transgenic Daphnia harboring the heat-shock responsive GFP reporter gene and confirmed that the GFP gene responds to heat treatment not only in juveniles and adults but also in embryos. We collected embryos from the reporter line and irradiated four different regions of interest in the embryos: a proximal region of the third thoracic segment, a part of the midline, a second maxilla, and a distal region of the endopodite of the second antenna, all of which increased GFP fluorescence with an infrared laser. Our results suggest that the IR-LEGO method is useful for spatial and temporal control of gene expression and would advance the functional genomics in D. magna.
Identifiants
pubmed: 39465323
doi: 10.1038/s41598-024-77458-8
pii: 10.1038/s41598-024-77458-8
doi:
Substances chimiques
HSP70 Heat-Shock Proteins
0
Green Fluorescent Proteins
147336-22-9
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
25696Subventions
Organisme : NIBB Collaborative Research Project for Integrative Imaging
ID : 22NIBB505, 21-405, 20-509, 19-511
Organisme : Japan Society for the Promotion of Science
ID : 23K21753, 21H03602
Organisme : Japan Society for the Promotion of Science
ID : 24H01367 , 23K23964, 23K18048, 22H05598, 22H02701, 20H04923, 19H05423
Informations de copyright
© 2024. The Author(s).
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