Emodin regulated lactate metabolism by inhibiting MCT1 to delay non-small cell lung cancer progression.
Monocarboxylic Acid Transporters
/ metabolism
Lung Neoplasms
/ pathology
Emodin
/ pharmacology
Humans
Symporters
/ metabolism
Carcinoma, Non-Small-Cell Lung
/ pathology
Disease Progression
Cell Proliferation
/ drug effects
Animals
Lactic Acid
/ metabolism
Mice, Nude
Disease Models, Animal
Cell Line, Tumor
Cell Movement
/ drug effects
Gene Expression
/ drug effects
Neoplasm Invasiveness
Thiophenes
/ pharmacology
Mice
Molecular Targeted Therapy
Pyrimidinones
Emodin
Lactate transport
Lung cancer
MCT1
Metabolism
Journal
Human cell
ISSN: 1749-0774
Titre abrégé: Hum Cell
Pays: Japan
ID NLM: 8912329
Informations de publication
Date de publication:
28 Oct 2024
28 Oct 2024
Historique:
received:
25
06
2024
accepted:
09
09
2024
medline:
28
10
2024
pubmed:
28
10
2024
entrez:
28
10
2024
Statut:
epublish
Résumé
Lung cancer is one of the most common malignant tumors in the world, with high incidence rate and mortality. Monocarboxylate transporter (MCT) 1 has been found to be widely expressed in various tumors and plays a crucial role in regulating energy metabolism. Emodin, as an important traditional Chinese medicine in China, has been reported to inhibit the progression of lung cancer. However, its potential mechanism has not been fully elucidated. The effects of emodin and MCT1 inhibitor AZD3965 on the proliferation, migration, and invasion of lung cancer cells were detected using cell counting kit-8 (CCK-8) assay, wound-healing assay, and transwell small chamber assay. The content of glucose, lactate, and pyruvate in the cell culture medium was detected using a glucose, lactate, and pyruvate detection kit, and also detected protein expression using western blotting. In addition, to investigate the effects of emodin and AZD3965 on lung cancer in vivo, we constructed nude mice subcutaneous transplant tumor model by subcutaneous injection of lung cancer cells. The results showed that emodin and AZD3965 could inhibit the proliferation, migration, and invasion of lung cancer cells. At the same time, they could inhibit the expression of MCT1 in lung cancer cells and promote the release of lactate, but did not affect the content of glucose and pyruvate. In vivo experiments had shown that emodin and AZD3965 could effectively inhibit the growth of lung cancer and inhibit the expression of MCT1. All in all, our data suggested that emodin inhibited the proliferation, migration, and invasion of lung cancer cells, possibly by inhibiting MCT1, providing important theoretical basis for elucidating the mechanism of emodin in treating lung cancer.
Identifiants
pubmed: 39465441
doi: 10.1007/s13577-024-01140-4
pii: 10.1007/s13577-024-01140-4
doi:
Substances chimiques
Monocarboxylic Acid Transporters
0
monocarboxylate transport protein 1
0
Emodin
KA46RNI6HN
Symporters
0
AZD3965
0
Lactic Acid
33X04XA5AT
Thiophenes
0
Pyrimidinones
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
11Subventions
Organisme : the National Natural Science Foundation of China
ID : 81560088
Organisme : the National Natural Science Foundation of China
ID : 82060850
Organisme : Science and Technology Program of Guizhou Province
ID : Qian Ke He-ZK (2022)520
Organisme : Science and Technology Program of Guizhou Province
ID : Qian Ke He Platform Talent [2020] 6016-2
Organisme : Science and Technology Program of Guizhou Provincial Health Commission
ID : gzwkj2021-056
Organisme : Science and Technology Program of Guizhou Provincial Health Commission
ID : gzwkj2023-453
Organisme : Science and Technology Program of Guizhou Provincial Health Commission
ID : gzwkj2024-318
Organisme : Science and Technology Program of Guiyang
ID : Zhu Ke He (2022)-4-3-3
Organisme : Guizhou Higher Education Engineering Research Center Project
ID : Qian Jiao Ji [2023] 037
Organisme : Guizhou Higher Education Engineering Research Center Project
ID : Qian Jiao Ji [2024] 125
Informations de copyright
© 2024. The Author(s) under exclusive licence to Japan Human Cell Society.
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