Biological characteristics and functions of a novel glutamate dehydrogenase from Trichinella spiralis.
Caractéristiques biologiques et fonctions d’une nouvelle glutamate déshydrogénase de Trichinella spiralis.
Glutamate dehydrogenase
Metabolism
Molting
RNA interference
Trichinella spiralis
Journal
Parasite (Paris, France)
ISSN: 1776-1042
Titre abrégé: Parasite
Pays: France
ID NLM: 9437094
Informations de publication
Date de publication:
2024
2024
Historique:
received:
28
05
2024
accepted:
30
09
2024
medline:
28
10
2024
pubmed:
28
10
2024
entrez:
28
10
2024
Statut:
ppublish
Résumé
Glutamate dehydrogenase (GDH) plays an important role in the metabolism of organisms. Its high abundance in mitochondria in particular highlights its core role in cellular physiological processes. GDH catalyzes the mutual conversion between L-glutamic acid and α-ketoglutaric acids. At the same time, this transformation is accompanied by the oxidation-reduction of NAD(H) or NADP(H). This process not only helps to link amino acid metabolism with sugar metabolism, but also helps maintain the balance of intracellular pH and nitrogen homeostasis. In this study, a novel Trichinella spiralis glutamate dehydrogenase (TsGDH) was cloned, expressed and identified. The results revealed that TsGDH was expressed at various stages of development of the nematode T. spiralis, with higher expression levels in the adult worm stage, and was mainly localized in the cuticle, muscular layer, stichosome and female intrauterine embryos. After RNAi treatment, larval natural TsGDH enzyme activity was obviously reduced, and metabolism, molting, growth and reproduction were also significantly inhibited. The results indicate that TsGDH plays an important role in the development and survival of T. spiralis, and it may be a potential molecular target of anti-Trichinella vaccines and drugs. Caractéristiques biologiques et fonctions d’une nouvelle glutamate déshydrogénase de Trichinella spiralis. La glutamate déshydrogénase (GDH) joue un rôle important dans le métabolisme des organismes. En particulier, sa forte abondance dans les mitochondries souligne son rôle essentiel dans les processus physiologiques cellulaires. La GDH catalyse la conversion mutuelle entre l’acide L-glutamique et les acides α-cétoglutariques. Dans le même temps, cette transformation s’accompagne de l’oxydoréduction du NAD(H) ou du NADP(H). Ce processus permet non seulement de lier le métabolisme des acides aminés au métabolisme du sucre, mais également de maintenir l’équilibre du pH intracellulaire et l’homéostasie de l’azote. Dans cette étude, une nouvelle glutamate déshydrogénase de Trichinella spiralis (TsGDH) a été clonée, exprimée et identifiée. Les résultats ont révélé que la TsGDH était exprimée à différents stades de développement du nématode T. spiralis, avec un niveau d’expression plus élevé au stade adulte du ver, et qu’elle est principalement localisée dans la cuticule, la couche musculaire, le stichosome et les embryons intra-utérins chez les femelles. Après traitement par ARN interférent, l’activité enzymatique naturelle de la TsGDH des larves était réduite, et le métabolisme, la mue, la croissance et la reproduction étaient également significativement inhibés. Les résultats indiquent que la TsGDH joue un rôle important dans le développement et la survie de T. spiralis, et qu’elle pourrait être une cible moléculaire potentielle pour un vaccin et des médicaments anti-Trichinella.
Autres résumés
Type: Publisher
(fre)
Caractéristiques biologiques et fonctions d’une nouvelle glutamate déshydrogénase de Trichinella spiralis.
Identifiants
pubmed: 39465975
doi: 10.1051/parasite/2024065
pii: parasite240102
doi:
Substances chimiques
Glutamate Dehydrogenase
EC 1.4.1.2
Helminth Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
65Subventions
Organisme : National Natural Science Foundation of China
ID : 82172300
Informations de copyright
© Y.K. Cheng et al., published by EDP Sciences, 2024.
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