Circulating HBsAg-specific B cells are partially rescued in chronically HBV-infected patients with functional cure.
B cells
B-cell epitope
Hepatitis B surface antigen
functional cure
hepatitis B surface antigen clearance
Journal
Emerging microbes & infections
ISSN: 2222-1751
Titre abrégé: Emerg Microbes Infect
Pays: United States
ID NLM: 101594885
Informations de publication
Date de publication:
Dec 2024
Dec 2024
Historique:
medline:
29
10
2024
pubmed:
29
10
2024
entrez:
29
10
2024
Statut:
ppublish
Résumé
It is well established that humoral immunity targeting hepatitis B virus surface antigen (HBsAg) plays a critical role in viral clearance and clinical cure. However, the functional changes in HBsAg-specific B cells before and after achieving functional cure remain poorly understood. In this study, we characterized circulating HBsAg-specific B cells and identified functional shifts and B-cell epitopes directly associated with HBsAg loss. The phenotypes and functions of HBV-specific B cells in patients with chronic HBV infection were investigated using a dual staining method and the ELISpot assay. Epitope mapping was performed to identify B cell epitopes associated with functional cure. Hyperactivated HBsAg-specific B cells in patients who achieved HBsAg loss were composed of enriched resting memory and contracted atypical memory fractions, accompanied by sustained co-expression of multiple inhibitory receptors and increased IL-6 secretion. The frequency of HBsAb-secreting B cells was significantly increased after achieving a functional cure. The rHBsAg displayed a weaker immunomodulatory effect on B cells than rHBeAg and rHBcAg
Identifiants
pubmed: 39470771
doi: 10.1080/22221751.2024.2409350
doi:
Substances chimiques
Hepatitis B Surface Antigens
0
Epitopes, B-Lymphocyte
0
Hepatitis B Antibodies
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
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