Identification of interaction partners of outer inflammatory protein A: Computational and experimental insights into how Helicobacter pylori infects host cells.
Helicobacter pylori
/ metabolism
Humans
Bacterial Outer Membrane Proteins
/ metabolism
Helicobacter Infections
/ microbiology
Epithelial Cells
/ microbiology
Hepatocyte Growth Factor
/ metabolism
Host-Pathogen Interactions
Proto-Oncogene Proteins c-met
/ metabolism
Bacterial Adhesion
Protein Binding
Proteomics
/ methods
Gastric Mucosa
/ microbiology
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2024
2024
Historique:
received:
01
03
2024
accepted:
26
09
2024
medline:
29
10
2024
pubmed:
29
10
2024
entrez:
29
10
2024
Statut:
epublish
Résumé
Outer membrane proteins (OMPs) play a key role in facilitating the survival of Helicobacter pylori within the gastric tissue by mediating adherence. Among these proteins, Outer inflammatory protein A (OipA) is a critical factor in H. pylori colonization of the host gastric epithelial cell surface. While the role of OipA in H. pylori attachment and its association with clinical outcomes have been established, the structural mechanisms underlying OipA's action in adherence to gastric epithelial cells remain limited. Our study employed experimental and computational approaches to investigate the interaction partners of OipA on the gastric epithelial cell surface. Initially, we conducted a proteomic analysis using a pull-down assay with recombinant OipA and gastric epithelial cell membrane proteins to identify the OipA interactome. This analysis revealed 704 unique proteins that interacted with OipA. We subsequently analyzed 16 of these OipA partners using molecular modeling tools. Among these 16 partners, we highlight three human proteins, namely Hepatocyte growth factor (HGF), Mesenchymal epithelial transition factor receptor (Met), and Adhesion G Protein-Coupled Receptor B1 (AGRB1) that could play a role in H. pylori adherence to the gastric epithelial cell surface with OipA. Collectively, these findings reveal novel host interactions mediated by OipA, suggesting their potential as therapeutic targets for combating H. pylori infection.
Identifiants
pubmed: 39471168
doi: 10.1371/journal.pone.0300557
pii: PONE-D-24-08191
pmc: PMC11521304
doi:
Substances chimiques
Bacterial Outer Membrane Proteins
0
OipA protein, Helicobacter pylori
0
Hepatocyte Growth Factor
67256-21-7
Proto-Oncogene Proteins c-met
EC 2.7.10.1
HGF protein, human
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0300557Informations de copyright
Copyright: © 2024 Akcelik-Deveci et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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