Rapid increase of MFGE8 secretion from endometrial epithelial cells is an indicator of extracellular vesicle mediated embryo maternal dialogue.
Embryo
Embryo Implantation
Endometrium
Extracellular Vesicles
Milk fat globule protein 8
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
29 10 2024
29 10 2024
Historique:
received:
02
02
2024
accepted:
09
10
2024
medline:
30
10
2024
pubmed:
30
10
2024
entrez:
30
10
2024
Statut:
epublish
Résumé
Successful embryo implantation relies on synchronized dialog between the embryo and endometrium, and the role of extracellular vesicles (EVs) in facilitating this cross-talk has been recently established. In our previous study, milk fat globule-EGF factor 8 protein (MFGE8) was identified as increasing in receptive endometrial epithelial cells (EECs) in response to trophoblastic EVs. However, the dynamics of MFGE8 protein in this context are not completely understood. Therefore, we examined its expression and secretion in EECs exposed to estrogen, progesterone, and trophoblastic EVs to gain deeper insights into its potential as an indicator of EV-mediated embryo-maternal dialogue. Our findings revealed that MFGE8 secretion is sensitive to estrogen and progesterone, and that trophoblastic EVs stimulate their release in both receptive and non-receptive EECs. Furthermore, trophoblast EV function was dose and time-dependent. Notably, the secretion of MFGE8 increased within a short timeframe of 30 min after addition of EVs, suggesting the possibility of rapid processes such as binding, fusion or internalization of trophoblastic EVs within EECs. Interestingly, MFGE8 released from EECs was associated with EVs, suggesting increased EV secretion from EECs in response to embryonic signals. In conclusion, increased MFGE8 secretion in this embryo implantation model can serve as an indicator of EV-mediated embryo-maternal dialogue.
Identifiants
pubmed: 39472639
doi: 10.1038/s41598-024-75893-1
pii: 10.1038/s41598-024-75893-1
doi:
Substances chimiques
Milk Proteins
0
MFGE8 protein, human
0
Progesterone
4G7DS2Q64Y
Antigens, Surface
0
Estrogens
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
25911Subventions
Organisme : European Union's Horizon 2020 research and innovation programme
ID : No 857418 COMBIVET
Organisme : European Union's Horizon 2020 research and innovation programme
ID : No 857418 COMBIVET
Organisme : European Union's Horizon 2020 research and innovation programme
ID : No 857418 COMBIVET
Organisme : European Union's Horizon 2020 research and innovation programme
ID : No 857418 COMBIVET
Organisme : European Union through Horizon coordination and support actions
ID : No. 101079349 OH-Boost
Organisme : European Union through Horizon coordination and support actions
ID : No. 101079349 OH-Boost
Organisme : European Union through Horizon coordination and support actions
ID : No. 101079349 OH-Boost
Organisme : European Union through Horizon coordination and support actions
ID : No. 101079349 OH-Boost
Informations de copyright
© 2024. The Author(s).
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