Evaluation of encapsulated Bacillus subtilis bio-mortars for use under acidic conditions.
Bacillus subtilis
Concrete
Mechanical properties
Microbially induced calcium carbonate precipitation (MICP)
Mortar
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
29 10 2024
29 10 2024
Historique:
received:
02
05
2024
accepted:
22
10
2024
medline:
30
10
2024
pubmed:
30
10
2024
entrez:
30
10
2024
Statut:
epublish
Résumé
This research aimed to examine the effects of an acidic environment on the mechanical properties and durability of bio-mortar (BM) encapsulated with Bacillus subtilis bacteria, in comparison to normal mortar (NM). The results at 28 days indicated that both 3% and 6% HCl significantly increased the compressive strength of the BM by 25% and 50%, respectively, compared with that of the NM. However, when 11% HCl was introduced, the compressive strength of the BM decreased to 50% lower than that of the NM. Furthermore, the water absorption rate of the BM was 33% lower than that of the NM. The mass loss for both 3% and 6% HCl was comparable, whereas at 11% HCl, BM experienced a mass loss that was 68% greater than that of NM. These findings suggest that with 3% and 6% HCl, the microbially induced calcium carbonate precipitation (MICP) process effectively generated CaCO
Identifiants
pubmed: 39472749
doi: 10.1038/s41598-024-77339-0
pii: 10.1038/s41598-024-77339-0
doi:
Substances chimiques
Calcium Carbonate
H0G9379FGK
Hydrochloric Acid
QTT17582CB
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
25947Informations de copyright
© 2024. The Author(s).
Références
1 Achal, V., Mukherjee, A. & Reddy, M. S. Microbial concrete: way to enhance the durability of building structures. J. Mater. Civ. Eng. 23, 730–734 (2011).
doi: 10.1061/(ASCE)MT.1943-5533.0000159
2 Issa, C. A. & Debs, P. Experimental study of epoxy repairing of cracks in concrete. Constr. Build. Mater. 21, 157–163 (2007).
doi: 10.1016/j.conbuildmat.2005.06.030
3 Zhang, B. et al. Modified cement-sodium silicate material and grouting technology for repairing underground concrete structure cracks. Arab. J. Geosci. 12, 1–10 (2019).
doi: 10.1007/s12517-019-4878-y
4 Bras, A., Gião, R., Lúcio, V. & Chastre, C. Development of an injectable grout for concrete repair and strengthening. Cem. Concr Compos. 37, 185–195 (2013).
doi: 10.1016/j.cemconcomp.2012.10.006
Castro-Alonso, M. J. et al. 5 Microbially induced calcium carbonate precipitation (MICP) and its potential in bioconcrete: microbiological and molecular concepts. Front. Mater. 6, 126 (2019).
6 Zhang, K. et al. Bin Shi. Microbial-induced carbonate precipitation (MICP) technology: a review on the fundamentals and engineering applications. Environ. Earth Sci. 82, 229 (2023).
doi: 10.1007/s12665-023-10899-y
pubmed: 37128499
pmcid: 10131530
7 Jiang, N. J., Yoshioka, H., Yamamoto, K. & Soga, K. Ureolytic activities of a urease-producing bacterium and purified urease enzyme in the anoxic condition: implication for subseafloor sand production control by microbially induced carbonate precipitation (MICP). Ecol. Eng. 90, 96–104 (2016).
doi: 10.1016/j.ecoleng.2016.01.073
8 Seifan, M., Samani, A. K. & Berenjian, A. Bioconcrete: next generation of self-healing concrete. Appl. Microbiol. Biotechnol. 100, 2591–2602 (2016).
doi: 10.1007/s00253-016-7316-z
pubmed: 26825821
9 Mokhtar, N., Johari, M. A. M., Tajarudin, H. A., Al-Gheethi, A. A. & Algaifi, H. A. A sustainable enhancement of bio-cement using immobilised Bacillus sphaericus: optimization, microstructural properties, and techno-economic analysis for a cleaner production of bio-cementitious mortars. J. Clean. Prod. 318, 128470 (2021).
doi: 10.1016/j.jclepro.2021.128470
Kovler, K., Roussel, N. & 10 & Properties of fresh and hardened concrete. Cem. Concr Res. 41, 775–792 (2011).
doi: 10.1016/j.cemconres.2011.03.009
11 Wang, J., Jonkers, H. M. & Boon, N. De Belie, N. Bacillus sphaericus LMG 22257 is physiologically suitable for self-healing concrete. Appl. Microbiol. Biotechnol. 101, 5101–5114 (2017).
doi: 10.1007/s00253-017-8260-2
pubmed: 28365797
12, Fajardo-Cavazos, P. & Nicholson, W. Bacillus endospores isolated from granite: close molecular relationships to globally distributed Bacillus spp. from endolithic and extreme environments. Appl. Environ. Microbiol. 72, 2856–2863 (2006).
doi: 10.1128/AEM.72.4.2856-2863.2006
13 Yamasamit, N. et al. Effect of Bacillus subtilis on mechanical and self-healing properties in mortar with different crack widths and curing conditions. Sci. Rep. 13, 7844 (2023).
doi: 10.1038/s41598-023-34837-x
pubmed: 37188710
pmcid: 10185526
14 Mutitu, K. D. et al. Effects of biocementation on some properties of cement-based materials incorporating Bacillus species bacteria–a review. J. Sustainable Cement-Based Mater. 8, 309–325 (2019).
doi: 10.1080/21650373.2019.1640141
15 Gauvry, E., Mathot, A. G., Couvert, O., Leguérinel, I. & Coroller, L. Effects of temperature, pH and water activity on the growth and the sporulation abilities of Bacillus subtilis BSB1. Int. J. Food Microbiol. 337, 108915 (2021).
doi: 10.1016/j.ijfoodmicro.2020.108915
pubmed: 33152569
16 Zhu, X., Wang, J., De Belie, N. & Boon, N. Complementing urea hydrolysis and nitrate reduction for improved microbially induced calcium carbonate precipitation. Appl. Microbiol. Biotechnol. 103, 8825–8838 (2019).
doi: 10.1007/s00253-019-10128-2
pubmed: 31637492
17 Wong, L. S. Microbial cementation of ureolytic bacteria from the genus Bacillus: a review of the bacterial application on cement-based materials for cleaner production. J. Clean. Prod. 93, 5–17 (2015).
doi: 10.1016/j.jclepro.2015.01.019
18 Dick, J. et al. Bio-deposition of a calcium carbonate layer on degraded limestone by Bacillus species. Biodegradation. 17, 357–367 (2006).
doi: 10.1007/s10532-005-9006-x
pubmed: 16491305
19 Fahimizadeh, M., Diane Abeyratne, A., Mae, L. S., Singh, R. R. & Pasbakhsh, P. Biological self-healing of cement paste and mortar by non-ureolytic bacteria encapsulated in alginate hydrogel capsules. Materials. 13, 3711 (2020).
doi: 10.3390/ma13173711
pubmed: 32842561
pmcid: 7504608
20 Wang, J. et al. Application of modified-alginate encapsulated carbonate producing bacteria in concrete: a promising strategy for crack self-healing. Front. Microbiol. 6, 1088 (2015).
doi: 10.3389/fmicb.2015.01088
pubmed: 26528254
pmcid: 4602304
21 Ali, M., Mukhtar, H. & Dufossé, L. Microbial calcite induction: a magic that fortifies and heals concrete. Int. J. Environ. Sci. 20, 1113–1134 (2023).
doi: 10.1007/s13762-022-03941-2
22 Soda, P. R. K. et al. Performance assessment of sustainable biocement mortar incorporated with bacteria-encapsulated cement-coated alginate beads. Constr. Build. Mater. 411, 134198 (2024).
doi: 10.1016/j.conbuildmat.2023.134198
23 Smitha, M. P., Suji, D., Shanthi, M. & Adesina, A. Application of bacterial biomass in biocementation process to enhance the mechanical and durability properties of concrete. Clean. Mater. 3, 100050 (2022).
doi: 10.1016/j.clema.2022.100050
Chuo, S. C. et al. Mohamad Ibrahim. Insights into the current trends in the utilization of bacteria for microbially induced calcium carbonate precipitation. Materials. 13(21), 4993 (2020).
doi: 10.3390/ma13214993
pubmed: 33167607
pmcid: 7664203
Schwantes-Cezario, N. et al. Effects of Bacillus subtilis biocementation on the mechanical properties of mortars. Rev. IBRACON Estrut Mater. 12, 31–38 (2019).
doi: 10.1590/s1983-41952019000100005
Scandiffio, P. et al. Anti-erosive effect of calcium carbonate suspensions. J. Clin. Exp. Dent. 10, e776 (2018).
pubmed: 30305876
pmcid: 6174013
Alvarez, J. et al. Methodology and validation of a hot hydrochloric acid attack for the characterization of ancient mortars. Cem. Concr. Res. 29.7, 1061–1065 (1999).
doi: 10.1016/S0008-8846(99)00090-3
Lu, C., Ge, H., Li, Z. & Zheng, Y. Effect evaluation of microbial mineralization for repairing load-induced crack in concrete with a cyclic injection-immersion process. Case Stud. Constr. Mater. 17, e01702 (2022).