GnRH antagonist impairs the process of embryo implantation by inhibiting motility of endometrial stromal cells through reducing c-kit expression.
Female
Humans
Gonadotropin-Releasing Hormone
/ antagonists & inhibitors
Stromal Cells
/ drug effects
Embryo Implantation
/ drug effects
Endometrium
/ drug effects
Adult
Cell Movement
/ drug effects
Proto-Oncogene Proteins c-kit
/ metabolism
Pregnancy
Hormone Antagonists
/ pharmacology
Retrospective Studies
Embryo Transfer
Fertilization in Vitro
/ methods
Animals
C-kit
GnRH-ant
IVF-ET
endometrial receptivity
motility
Journal
Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology
ISSN: 1473-0766
Titre abrégé: Gynecol Endocrinol
Pays: England
ID NLM: 8807913
Informations de publication
Date de publication:
Dec 2024
Dec 2024
Historique:
medline:
1
11
2024
pubmed:
1
11
2024
entrez:
1
11
2024
Statut:
ppublish
Résumé
It has been recognized that the gonadotropin-releasing hormone antagonist (GnRH-ant) protocol has a detrimental effect on clinical outcomes compared to the GnRH agonist (GnRH-a) protocol during A total of 18,561 patients, who underwent fresh IVF-ET cycles in the Center for Assisted Reproduction of Jiangxi Maternal and Child Health Hospital from January 2014 to September 2021, were retrospectively analyzed. The propensity score matching (PSM) technique was used to control for confounding factors between the GnRH-ant and GnRH-a groups. Human endometrial stromal cells (hESCs) were collected for primary culture and treated with relevant receptor antagonists and activators. RT-PCR, Western Blot, immunofluorescence staining, cell migration and adhesion assays, and animal experiments were employed to elucidate the molecular mechanism by which GnRH antagonist affects the migration and adhesion ability of hESCs. There was no statistical difference between the two groups in terms of baseline characteristics after matching basal status by propensity score matching. The result showed that the endometrial thickness (10.4 ± 2.35 GnRH-ant could reduce the motility of hESCs by inhibiting the expression and activation of the C-kit receptor, which impaired the process of embryo implantation.
Sections du résumé
BACKGROUND
UNASSIGNED
It has been recognized that the gonadotropin-releasing hormone antagonist (GnRH-ant) protocol has a detrimental effect on clinical outcomes compared to the GnRH agonist (GnRH-a) protocol during
METHODS
UNASSIGNED
A total of 18,561 patients, who underwent fresh IVF-ET cycles in the Center for Assisted Reproduction of Jiangxi Maternal and Child Health Hospital from January 2014 to September 2021, were retrospectively analyzed. The propensity score matching (PSM) technique was used to control for confounding factors between the GnRH-ant and GnRH-a groups. Human endometrial stromal cells (hESCs) were collected for primary culture and treated with relevant receptor antagonists and activators. RT-PCR, Western Blot, immunofluorescence staining, cell migration and adhesion assays, and animal experiments were employed to elucidate the molecular mechanism by which GnRH antagonist affects the migration and adhesion ability of hESCs.
RESULTS
UNASSIGNED
There was no statistical difference between the two groups in terms of baseline characteristics after matching basal status by propensity score matching. The result showed that the endometrial thickness (10.4 ± 2.35
CONCLUSIONS
UNASSIGNED
GnRH-ant could reduce the motility of hESCs by inhibiting the expression and activation of the C-kit receptor, which impaired the process of embryo implantation.
Identifiants
pubmed: 39485323
doi: 10.1080/09513590.2024.2421487
doi:
Substances chimiques
Gonadotropin-Releasing Hormone
33515-09-2
Proto-Oncogene Proteins c-kit
EC 2.7.10.1
Hormone Antagonists
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM