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"description": "Quelles complications peuvent survenir avec le profilage d'ADN ?\nLe profilage d'ADN peut-il entraîner des erreurs d'identification ?\nY a-t-il des risques de confidentialité avec le profilage d'ADN ?\nLe profilage d'ADN peut-il causer des conflits familiaux ?\nQuelles sont les implications légales du profilage d'ADN ?",
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"description": "Quels facteurs influencent la nécessité de profilage d'ADN ?\nLe profilage d'ADN est-il plus fréquent dans certaines populations ?\nLes antécédents médicaux influencent-ils le profilage d'ADN ?\nY a-t-il des facteurs environnementaux liés au profilage d'ADN ?\nLe mode de vie affecte-t-il le besoin de profilage d'ADN ?",
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"@type": "Question",
"name": "Comment le profilage d'ADN est-il utilisé dans le diagnostic ?",
"position": 1,
"acceptedAnswer": {
"@type": "Answer",
"text": "Il permet d'identifier des individus dans des enquêtes criminelles ou des tests de paternité."
}
},
{
"@type": "Question",
"name": "Quels échantillons sont nécessaires pour le profilage d'ADN ?",
"position": 2,
"acceptedAnswer": {
"@type": "Answer",
"text": "Des échantillons de sang, de salive ou de cheveux peuvent être utilisés."
}
},
{
"@type": "Question",
"name": "Le profilage d'ADN peut-il aider à identifier des maladies héréditaires ?",
"position": 3,
"acceptedAnswer": {
"@type": "Answer",
"text": "Oui, il peut révéler des prédispositions génétiques à certaines maladies."
}
},
{
"@type": "Question",
"name": "Le profilage d'ADN est-il fiable ?",
"position": 4,
"acceptedAnswer": {
"@type": "Answer",
"text": "Oui, il est considéré comme très fiable avec un faible taux d'erreur."
}
},
{
"@type": "Question",
"name": "Peut-on utiliser le profilage d'ADN pour des tests de parenté ?",
"position": 5,
"acceptedAnswer": {
"@type": "Answer",
"text": "Oui, il est couramment utilisé pour établir des liens de parenté."
}
},
{
"@type": "Question",
"name": "Quels symptômes peuvent indiquer un besoin de profilage d'ADN ?",
"position": 6,
"acceptedAnswer": {
"@type": "Answer",
"text": "Il n'y a pas de symptômes physiques, mais des situations légales ou familiales peuvent l'exiger."
}
},
{
"@type": "Question",
"name": "Le profilage d'ADN peut-il révéler des anomalies génétiques ?",
"position": 7,
"acceptedAnswer": {
"@type": "Answer",
"text": "Indirectement, il peut indiquer des risques génétiques, mais ce n'est pas son but principal."
}
},
{
"@type": "Question",
"name": "Y a-t-il des symptômes psychologiques liés à l'utilisation de l'ADN ?",
"position": 8,
"acceptedAnswer": {
"@type": "Answer",
"text": "Des résultats inattendus peuvent causer du stress ou de l'anxiété chez les individus."
}
},
{
"@type": "Question",
"name": "Le profilage d'ADN peut-il aider à diagnostiquer des maladies ?",
"position": 9,
"acceptedAnswer": {
"@type": "Answer",
"text": "Il peut aider à identifier des prédispositions, mais ne diagnostique pas directement des maladies."
}
},
{
"@type": "Question",
"name": "Quels impacts émotionnels le profilage d'ADN peut-il avoir ?",
"position": 10,
"acceptedAnswer": {
"@type": "Answer",
"text": "Il peut provoquer des révélations sur l'identité ou des liens familiaux, entraînant des émotions fortes."
}
},
{
"@type": "Question",
"name": "Le profilage d'ADN peut-il aider à la prévention des maladies ?",
"position": 11,
"acceptedAnswer": {
"@type": "Answer",
"text": "Oui, il peut identifier des risques génétiques, permettant des mesures préventives."
}
},
{
"@type": "Question",
"name": "Comment le profilage d'ADN est-il utilisé en santé publique ?",
"position": 12,
"acceptedAnswer": {
"@type": "Answer",
"text": "Il aide à surveiller les maladies génétiques et à planifier des interventions de santé."
}
},
{
"@type": "Question",
"name": "Le profilage d'ADN peut-il influencer le mode de vie ?",
"position": 13,
"acceptedAnswer": {
"@type": "Answer",
"text": "Oui, les résultats peuvent inciter à adopter des comportements plus sains."
}
},
{
"@type": "Question",
"name": "Y a-t-il des programmes de prévention basés sur le profilage d'ADN ?",
"position": 14,
"acceptedAnswer": {
"@type": "Answer",
"text": "Certaines initiatives de santé utilisent le profilage pour cibler des populations à risque."
}
},
{
"@type": "Question",
"name": "Le profilage d'ADN est-il accessible pour la prévention ?",
"position": 15,
"acceptedAnswer": {
"@type": "Answer",
"text": "L'accès varie selon les pays et les systèmes de santé, mais il devient plus courant."
}
},
{
"@type": "Question",
"name": "Le profilage d'ADN est-il utilisé pour des traitements médicaux ?",
"position": 16,
"acceptedAnswer": {
"@type": "Answer",
"text": "Non, il n'est pas un traitement, mais peut guider des décisions thérapeutiques."
}
},
{
"@type": "Question",
"name": "Comment le profilage d'ADN influence-t-il les traitements ?",
"position": 17,
"acceptedAnswer": {
"@type": "Answer",
"text": "Il peut aider à choisir des traitements adaptés en fonction des prédispositions génétiques."
}
},
{
"@type": "Question",
"name": "Le profilage d'ADN peut-il prédire l'efficacité d'un traitement ?",
"position": 18,
"acceptedAnswer": {
"@type": "Answer",
"text": "Oui, il peut indiquer comment un patient réagira à certains médicaments."
}
},
{
"@type": "Question",
"name": "Y a-t-il des traitements spécifiques basés sur le profilage d'ADN ?",
"position": 19,
"acceptedAnswer": {
"@type": "Answer",
"text": "Certains traitements ciblés en oncologie utilisent des informations génétiques."
}
},
{
"@type": "Question",
"name": "Le profilage d'ADN est-il utilisé dans la recherche de nouveaux traitements ?",
"position": 20,
"acceptedAnswer": {
"@type": "Answer",
"text": "Oui, il est essentiel pour identifier des cibles thérapeutiques dans la recherche."
}
},
{
"@type": "Question",
"name": "Quelles complications peuvent survenir avec le profilage d'ADN ?",
"position": 21,
"acceptedAnswer": {
"@type": "Answer",
"text": "Des complications éthiques et psychologiques peuvent survenir, comme la stigmatisation."
}
},
{
"@type": "Question",
"name": "Le profilage d'ADN peut-il entraîner des erreurs d'identification ?",
"position": 22,
"acceptedAnswer": {
"@type": "Answer",
"text": "Bien que rare, des erreurs peuvent se produire, entraînant des conséquences juridiques."
}
},
{
"@type": "Question",
"name": "Y a-t-il des risques de confidentialité avec le profilage d'ADN ?",
"position": 23,
"acceptedAnswer": {
"@type": "Answer",
"text": "Oui, la confidentialité des données génétiques est une préoccupation majeure."
}
},
{
"@type": "Question",
"name": "Le profilage d'ADN peut-il causer des conflits familiaux ?",
"position": 24,
"acceptedAnswer": {
"@type": "Answer",
"text": "Oui, des résultats inattendus peuvent révéler des secrets familiaux, entraînant des tensions."
}
},
{
"@type": "Question",
"name": "Quelles sont les implications légales du profilage d'ADN ?",
"position": 25,
"acceptedAnswer": {
"@type": "Answer",
"text": "Il peut soulever des questions juridiques concernant la propriété des données et l'utilisation."
}
},
{
"@type": "Question",
"name": "Quels facteurs influencent la nécessité de profilage d'ADN ?",
"position": 26,
"acceptedAnswer": {
"@type": "Answer",
"text": "Des facteurs comme l'hérédité, l'histoire familiale et des enquêtes criminelles peuvent influencer."
}
},
{
"@type": "Question",
"name": "Le profilage d'ADN est-il plus fréquent dans certaines populations ?",
"position": 27,
"acceptedAnswer": {
"@type": "Answer",
"text": "Oui, certaines populations peuvent être plus souvent testées en raison de prédispositions génétiques."
}
},
{
"@type": "Question",
"name": "Les antécédents médicaux influencent-ils le profilage d'ADN ?",
"position": 28,
"acceptedAnswer": {
"@type": "Answer",
"text": "Oui, des antécédents familiaux de maladies peuvent justifier un profilage d'ADN."
}
},
{
"@type": "Question",
"name": "Y a-t-il des facteurs environnementaux liés au profilage d'ADN ?",
"position": 29,
"acceptedAnswer": {
"@type": "Answer",
"text": "Des facteurs environnementaux peuvent interagir avec des prédispositions génétiques, influençant la santé."
}
},
{
"@type": "Question",
"name": "Le mode de vie affecte-t-il le besoin de profilage d'ADN ?",
"position": 30,
"acceptedAnswer": {
"@type": "Answer",
"text": "Oui, un mode de vie à risque peut inciter à un profilage pour évaluer des risques génétiques."
}
}
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}
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}
The clinical course of ischemic and hemorrhagic strokes can be influenced by the coagulation status of individual patients. The prior use of antiplatelet therapy (APT) such as acetylsalicylic acid (AS...
We used fluorescence-activated cell sorting (FACS) with antibodies against CD61 for thrombocyte identification and CD62p or platelet activation complex-1 (PAC-1) to determine platelet activation. Aggr...
Between February and July 2023, 20 blood samples (...
In the clinical setting it is feasible to use differentiated platelet analytics to determine alterations caused by antiplatelet therapy. Among the tests under investigation, PFA-200 showed the highest...
This systematic review aims to comprehensively assess the contemporary literature on platelet function testing (PFT) in individuals undergoing revascularization therapy for peripheral arterial disease...
Following PRISMA guidelines, a literature review was conducted using PubMed databases. Search terms included relevant medical subject headings (MeSH) terms. Eligible articles published in English betw...
Ten studies met the inclusion criteria. Various PFT methods were used, including Thromboelastography with Platelet Mapping (TEG-PM), Multiplate Analyzer, Cytochrome P450 2C19 testing, VerifyNow, Corre...
PFTs can play a crucial role in detecting resistance and non-sensitivity to antiplatelet drugs in PAD patients post-revascularization. However, heterogeneity of data and methods underlines the need fo...
In hemophilia and von Willebrand disease, the degree of alteration of laboratory assays correlates with bleeding manifestations. Few studies have assessed the predictive value for bleeding of laborato...
To assess whether there is an association between platelet function assay results and bleeding history, as evaluated by the International Society on Thrombosis and Haemostasis (ISTH) bleeding assessme...
Centers participating in the international ISTH-BAT validation study were asked to provide results of the diagnostic assays employed for the patients they enrolled, and the association with the indivi...
Sixty-eight patients with 14 different IPFDs were included. Maximal amplitude of platelet aggregation was significantly lower in patients with a pathologic BS and correlated inversely with the BS, a f...
This study shows that altered platelet laboratory assay results are associated with an abnormal ISTH-BAT BS in IPFD....
Cyclooxygenase (COX) isozymes, i.e., COX-1 and COX-2, are encoded by separate genes and are involved in the generation of the same products, prostaglandin (PG)G...
Light transmission aggregometry (LTA) is important for diagnosing platelet function disorders (PFD) and von Willebrand disease (VWD) affecting ristocetin-induced platelet aggregation (RIPA). Nonethele...
To further evaluate the L-PRP LTA strategy, we evaluated findings for a subsequent patient cohort....
Between 2008 and 2021, the L-PRP strategy was applied to 211 samples (11.7% of all LTA samples) from 192 unique patients, whose platelet counts (median [range] × 10...
Diagnostic LTA with L-PRP, using a strategy that considers thrombocytopenia severity, is feasible and informative....
Platelet function testing has been proposed to better adjust individualized antiplatelet treatment for patients undergoing endovascular treatment for intracranial aneurysms. Its clinical significance ...
Our aim was to evaluate the impact of platelet function testing-guided versus standard antiplatelet treatment in patients receiving endovascular treatment for intracranial aneurysms....
PubMed, EMBASE, and the Cochrane Library of clinical trials were searched from inception until March 2023....
Eleven studies comprising 6199 patients were included....
ORs with 95% CIs were calculated using random effects models....
The platelet function testing-guided group was associated with a decreased rate of symptomatic thromboembolic events (OR = 0.57; 95% CI, 0.42-0.76; I...
Heterogeneous endovascular treatment methods and adjusted antiplatelet regimens were limitations....
Platelet function testing-guided antiplatelet strategy significantly reduced the incidence of symptomatic thromboembolic events without any increase in the hemorrhagic events for patients undergoing e...
Functional platelet activation assays are required for the diagnosis of heparin-induced thrombocytopenia (HIT). Due to their sophisticated methodology, they are only available in reference centers....
To evaluate the diagnostic accuracy of the flow cytometry-based heparin-activated procoagulant platelet (HAPP) assay in the laboratory diagnosis of HIT....
Procoagulant platelets (PCP), defined by the expression of phosphatidylserine and CD62-P, were evaluated by flow cytometry in platelet-rich plasma from healthy donors after incubation with patient ser...
HIT-positive sera induced PCPs in the presence of 0.2 IU/mL heparin, which was inhibited with 100 IU/mL of heparin. In the prospective validation cohort, there were 15 HIPA+ and 187 HIPA- sera. HAPP w...
HAPP assay is promising as a simple and reliable functional assay for HIT; however, further studies are needed to confirm our results in larger cohorts....
Platelet count alone does not reliably predict bleeding risk, suggesting platelet function is important to monitor in patients with thrombocytopenia. There is still an unmet need for improved platelet...
The goal of this joint project between the International Society on Thrombosis and Haemostasis (ISTH) Scientific Standardization Committee (SSC) Subcommittees on Platelet Physiology and Platelet Immun...
A literature review was performed to identify relevant questions and areas of interest. An electronic expression of interest form was thereafter announced on the ISTH webpage, followed by a survey enc...
Consensus recommendations relative to patient sample collection, preanalytical variables, sample type, platelet-count cutoff, any potential specific modification of the standard flow cytometry protoco...
The proposed consensus recommendations would allow standardization of protocols in upcoming clinical studies. The clinical utility of platelet function testing using flow cytometry to predict bleeding...
Endocytosis constitutes a cellular process in which cells selectively encapsulate surface substances into endocytic vesicles, also known as endosomes, thereby modulating their interaction with the env...
Recently the US Food and Drug Administration has granted variances to select blood centers to supply cold-stored platelet components (CSP). In hemorrhage resuscitation warming of blood components with...
Pathogen-reduced apheresis platelet units were collected and stored in one of two ways: (1) CSP-I, (2) CSP-D. CSP-I were collected and immediately stored at 1-6°C until used. CSP-D were collected and ...
CSP-I, 23 units, had very high aggregation responses to all agonists (all ≥47.6 ± 20.7). There was a statistically significant reduction in ADP-induced aggregometry results from 55.1 ± 23.2 before com...
We detected a statistically significant reduction in ADP-induced aggregometry in CSP-I run through a Ranger blood-warming device with no change with AA or collagen agonist aggregometry....