ZBED6 negatively regulates insulin production, neuronal differentiation, and cell aggregation in MIN6 cells.
Animals
Binding Sites
Cell Adhesion
Cell Aggregation
Cell Differentiation
Gene Expression Regulation
Gene Silencing
Glucose
/ administration & dosage
High-Throughput Nucleotide Sequencing
Insulin
/ metabolism
Insulin-Secreting Cells
/ metabolism
Insulinoma
/ metabolism
Mice
Neurons
/ metabolism
Pancreatic Neoplasms
/ metabolism
Repressor Proteins
/ antagonists & inhibitors
Transcription, Genetic
Tumor Cells, Cultured
ChIP-seq
cell adhesion
transcriptome analysis
β-cells
Journal
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
ISSN: 1530-6860
Titre abrégé: FASEB J
Pays: United States
ID NLM: 8804484
Informations de publication
Date de publication:
01 2019
01 2019
Historique:
pubmed:
30
6
2018
medline:
10
7
2019
entrez:
30
6
2018
Statut:
ppublish
Résumé
Zinc finger BED domain containing protein 6 ( Zbed6) has evolved from a domesticated DNA transposon and encodes a transcription factor unique to placental mammals. The aim of the present study was to investigate further the role of ZBED6 in insulin-producing cells, using mouse MIN6 cells, and to evaluate the effects of Zbed6 knockdown on basal β-cell functions, such as morphology, transcriptional regulation, insulin content, and release. Zbed6-silenced cells and controls were characterized with a range of methods, including RNA sequencing, chromatin immunoprecipitation sequencing, insulin content and release, subplasma membrane Ca
Identifiants
pubmed: 29957057
doi: 10.1096/fj.201600835R
doi:
Substances chimiques
Insulin
0
Repressor Proteins
0
ZBED6 protein, mouse
0
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM