Correlation between relative bacterial activity and lactate dehydrogenase gene expression of co-cultures in vitro.
16S rRNA
Biofilm
Dental caries/etiology
Fusobacteria
Lactobacilli
Relative bacterial activity
ldh-expression
Journal
Clinical oral investigations
ISSN: 1436-3771
Titre abrégé: Clin Oral Investig
Pays: Germany
ID NLM: 9707115
Informations de publication
Date de publication:
Mar 2019
Mar 2019
Historique:
received:
02
11
2017
accepted:
27
06
2018
pubmed:
8
7
2018
medline:
19
12
2019
entrez:
8
7
2018
Statut:
ppublish
Résumé
The present study aims at correlating the relative bacterial activity with the H Well plates were prepared with BHI medium and cultures of Lactobacillus paracasei and Fusobacterium nucleatum. Bacterial growth at 37 °C was measured using a microplate-photometer before and after adding sucrose to the samples. Samples of co-cultures (n = 12) and single-species cultures (n = 3) were taken and pH was assessed. Real-time quantitative PCRs were applied targeting the 16S-gene, the 16S-rRNA, the ldh-gene, and the ldh-mRNA. For L. paracasei with sucrose, an increase in relative bacterial activity (62.8% ± 23.5% [mean, SE]) was observed, while F. nucleatum showed a clear decrease in relative bacterial activity (- 35.0% ± 9.6%). Simultaneously, the H The relative bacterial activity after sucrose pulse showed a significant correlation not only to the acid production (H
Identifiants
pubmed: 29980934
doi: 10.1007/s00784-018-2547-2
pii: 10.1007/s00784-018-2547-2
doi:
Substances chimiques
L-Lactate Dehydrogenase
EC 1.1.1.27
Types de publication
Journal Article
Langues
eng
Pagination
1225-1235Références
Appl Environ Microbiol. 2002 Jan;68(1):114-23
pubmed: 11772617
Oral Microbiol Immunol. 2003 Apr;18(2):109-13
pubmed: 12654101
Caries Res. 2004 May-Jun;38(3):182-91
pubmed: 15153687
J Clin Microbiol. 2005 Feb;43(2):843-9
pubmed: 15695690
J Clin Microbiol. 2005 Nov;43(11):5721-32
pubmed: 16272510
FEMS Microbiol Lett. 2006 Apr;257(2):182-8
pubmed: 16553851
Periodontol 2000. 2006;42:80-7
pubmed: 16930307
Microbiology. 2007 Jan;153(Pt 1):148-59
pubmed: 17185543
J Bacteriol. 2010 Oct;192(19):5002-17
pubmed: 20656903
J Clin Microbiol. 2010 Nov;48(11):4121-8
pubmed: 20826648
J Dent Res. 2011 Mar;90(3):294-303
pubmed: 20924061
J Bacteriol. 1990 Oct;172(10):5714-23
pubmed: 2211506
PLoS One. 2012;7(10):e47722
pubmed: 23091642
J Clin Microbiol. 1990 Feb;28(2):319-23
pubmed: 2312676
Caries Res. 2013;47(1):69-77
pubmed: 23147531
Caries Res. 2013;47(2):89-102
pubmed: 23207320
J Oral Microbiol. 2014 Apr 23;6:null
pubmed: 24778776
Caries Res. 2014;48(5):451-60
pubmed: 24852305
PLoS One. 2014 Aug 01;9(8):e103712
pubmed: 25083880
Microbiologyopen. 2014 Oct;3(5):729-51
pubmed: 25155235
Appl Environ Microbiol. 2015 Feb;81(4):1267-73
pubmed: 25501477
J Oral Microbiol. 2014 Oct 24;6:25443
pubmed: 25626770
J Dent Res. 2015 Sep;94(9 Suppl):110S-8S
pubmed: 25758458
Anaerobe. 2015 Oct;35(Pt A):60-5
pubmed: 25933689
PLoS One. 2015 May 05;10(5):e0124631
pubmed: 25942317
Lancet. 2015 Aug 22;386(9995):743-800
pubmed: 26063472
Proteomics. 2015 Oct;15(20):3497-507
pubmed: 26272225
J Dent Res. 2015 Dec;94(12):1628-37
pubmed: 26377570
Caries Res. 2016;50(4):422-31
pubmed: 27458979
J Oral Microbiol. 2016 Jul 29;8:32513
pubmed: 27476444
Caries Res. 2016;50(5):480-488
pubmed: 27595541
Clin Oral Investig. 2017 Jun;21(5):1769-1776
pubmed: 27660160
Nucleic Acids Res. 1989 Oct 11;17(19):7843-53
pubmed: 2798131
J Clin Periodontol. 2017 Mar;44 Suppl 18:S5-S11
pubmed: 28266109
J Dent Res. 2017 Jul;96(7):733-740
pubmed: 28384412
Int J Oral Sci. 2017 Jun;9(2):74-79
pubmed: 28452377
Front Microbiol. 2017 Jun 08;8:1036
pubmed: 28642749
J Dent Res. 2017 Nov;96(12):1378-1385
pubmed: 28709393
J Bacteriol. 1987 Sep;169(9):3891-7
pubmed: 3114229
Antonie Van Leeuwenhoek. 1983 Sep;49(3):209-24
pubmed: 6354079
J Dent Res. 1994 Dec;73(12):1853-7
pubmed: 7814758
Clin Microbiol Rev. 1996 Jan;9(1):55-71
pubmed: 8665477
Caries Res. 1998;32(6):456-62
pubmed: 9745120