Brassica rapa hairy root based expression system leads to the production of highly homogenous and reproducible profiles of recombinant human alpha-L-iduronidase.
Animals
Brassica rapa
/ enzymology
CHO Cells
Cricetulus
Epitopes
/ immunology
Fucose
/ immunology
Glycosylation
Humans
Iduronidase
/ chemistry
Mannose
/ metabolism
Plant Roots
/ enzymology
Plants, Genetically Modified
Polysaccharides
/ metabolism
Recombinant Proteins
Reproducibility of Results
Transgenes
Xylose
/ immunology
alpha-L-iduronidase
glycoprotein
glycosylation
hairy root
plant based expression system
post-translational modification
recombinant
Journal
Plant biotechnology journal
ISSN: 1467-7652
Titre abrégé: Plant Biotechnol J
Pays: England
ID NLM: 101201889
Informations de publication
Date de publication:
02 2019
02 2019
Historique:
received:
18
01
2018
revised:
02
05
2018
accepted:
22
07
2018
pubmed:
31
7
2018
medline:
4
6
2019
entrez:
31
7
2018
Statut:
ppublish
Résumé
The Brassica rapa hairy root based expression platform, a turnip hairy root based expression system, is able to produce human complex glycoproteins such as the alpha-L-iduronidase (IDUA) with an activity similar to the one produced by Chinese Hamster Ovary (CHO) cells. In this article, a particular attention has been paid to the N- and O-glycosylation that characterize the alpha-L-iduronidase produced using this hairy root based system. This analysis showed that the recombinant protein is characterized by highly homogeneous post translational profiles enabling a strong batch to batch reproducibility. Indeed, on each of the 6 N-glycosylation sites of the IDUA, a single N-glycan composed of a core Man
Identifiants
pubmed: 30058762
doi: 10.1111/pbi.12994
pmc: PMC6335068
doi:
Substances chimiques
Epitopes
0
Polysaccharides
0
Recombinant Proteins
0
Fucose
28RYY2IV3F
Xylose
A1TA934AKO
IDUA protein, human
EC 3.2.1.76
Iduronidase
EC 3.2.1.76
Mannose
PHA4727WTP
Banques de données
GENBANK
['NP_000194.2']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
505-516Subventions
Organisme : BPI France
Pays : International
Organisme : Région Hauts de France
Pays : International
Informations de copyright
© 2018 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.
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