16S ribosomal RNA sequencing reveals a modulation of intestinal microbiome and immune response by dietary L-theanine supplementation in broiler chickens.
Animal Feed
/ analysis
Animals
Chickens
/ immunology
Diet
/ veterinary
Dietary Supplements
/ analysis
Dose-Response Relationship, Drug
Gastrointestinal Microbiome
/ drug effects
Glutamates
/ administration & dosage
Immunity, Innate
/ drug effects
RNA, Bacterial
/ analysis
RNA, Ribosomal, 16S
/ analysis
Random Allocation
Sequence Analysis, RNA
/ veterinary
16S rRNA
L-theanine
broiler
gut microbiota
immunity
Journal
Poultry science
ISSN: 1525-3171
Titre abrégé: Poult Sci
Pays: England
ID NLM: 0401150
Informations de publication
Date de publication:
01 Feb 2019
01 Feb 2019
Historique:
received:
20
02
2018
accepted:
03
08
2018
pubmed:
1
9
2018
medline:
7
3
2019
entrez:
1
9
2018
Statut:
ppublish
Résumé
Despite the availability of abundant literature on green tea, studies on the use of L-theanine (an amino acid found only in green tea) as a feed additive in poultry especially broiler are limited. So, this study was conducted to explore the effects of L-theanine on the intestinal microbiome and immune response in a broiler. A total of 400-d-old chicks were randomly divided into four treatment groups (A, B, C, and D) using a complete randomized design. Treatments were as follows: A, control (basal diet); B, basal diet + 100 mg L-theanine/kg diet; C, basal diet + 200 mg L-theanine/kg diet; and D, basal diet + 300 mg L-theanine/kg diet. Mucosal samples from ileum and jejunum of broiler chicken were extracted at 21 and 42 d of age. Extraction of genomic DNA was followed by amplification of V3 and V4 hypervariable regions of 16S ribosomal RNA. After Illumina sequencing, results revealed that treatment with L-theanine significantly increased the population of Lactobacillus in ileum and jejunum as compared to a control group, but the higher population was observed in jejunum at both 21 and 42 d of age. The overall diversity of the jejunum microbiome in the treatment group was significantly lower than that of the ileum and control group (P < 0.05). Results of this study revealed that mRNA expression of TLRs (TLR-2 and TLR-4) and cytokines (TNF-α, IFN-γ, and IL-2) was decreased in response to treatment with L-theanine. Moreover, the negative correlation of abundance of Lactobacillus was observed with expression of IL-2 and IFN-γ in the intestine and these effects were highly significant (P < 0.01). In summary, our finding revealed that dietary supplementation of L-theanine exhibited a positive influence on intestinal bacteria by supporting beneficial microbes like Lactobacillus while decreasing harmful microbes like Clostridium.
Identifiants
pubmed: 30169691
pii: S0032-5791(19)30558-9
doi: 10.3382/ps/pey394
pmc: PMC7107316
doi:
Substances chimiques
Glutamates
0
RNA, Bacterial
0
RNA, Ribosomal, 16S
0
theanine
8021PR16QO
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
842-854Informations de copyright
© 2018 Poultry Science Association Inc.
Références
Appl Environ Microbiol. 2009 Dec;75(23):7537-41
pubmed: 19801464
Nat Rev Microbiol. 2013 Apr;11(4):227-38
pubmed: 23435359
PLoS One. 2008 Aug 13;3(8):e2945
pubmed: 18698407
Cell. 2014 Mar 27;157(1):121-41
pubmed: 24679531
J Nutr. 2012 Dec;142(12):2091-6
pubmed: 23096008
Comp Immunol Microbiol Infect Dis. 2008 Sep;31(5):389-402
pubmed: 17686519
Int J Syst Bacteriol. 1995 Oct;45(4):780-2
pubmed: 7547299
FEMS Microbiol Ecol. 2007 Jan;59(1):147-57
pubmed: 17233749
Appl Environ Microbiol. 2003 Nov;69(11):6816-24
pubmed: 14602645
Arch Anim Nutr. 2007 Oct;61(5):319-35
pubmed: 18030916
Biochim Biophys Acta. 2003 Mar 17;1620(1-3):47-53
pubmed: 12595072
Poult Sci. 2007 Nov;86(11):2396-403
pubmed: 17954591
Appl Environ Microbiol. 2002 Dec;68(12):5918-24
pubmed: 12450811
Biochem Biophys Res Commun. 2012 Jun 1;422(2):344-50
pubmed: 22583898
J Innate Immun. 2009;1(2):123-35
pubmed: 20375571
Gut Pathog. 2015 Feb 26;7:4
pubmed: 25806087
Science. 2003 Oct 10;302(5643):276-8
pubmed: 12958366
Poult Sci. 2007 Jun;86(6):1100-6
pubmed: 17495079
J Nutr. 2008 Nov;138(11):2264-8
pubmed: 18936229
Anim Health Res Rev. 2008 Jun;9(1):101-10
pubmed: 18541076
Annu Rev Nutr. 2002;22:283-307
pubmed: 12055347
Physiol Rev. 2001 Jul;81(3):1031-64
pubmed: 11427691
Science. 2010 Dec 24;330(6012):1768-73
pubmed: 21205662
Poult Sci. 2012 Jun;91(6):1335-41
pubmed: 22582290
Cell Microbiol. 2008 May;10(5):1093-103
pubmed: 18088401
Gut Microbes. 2010 Jan;1(1):51-54
pubmed: 21327117
Immunopharmacol Immunotoxicol. 2014 Oct;36(5):364-70
pubmed: 25151997
Front Microbiol. 2016 Feb 19;7:187
pubmed: 26925052
Nat Rev Cancer. 2004 Jan;4(1):45-60
pubmed: 14681689
Poult Sci. 2003 Aug;82(8):1242-9
pubmed: 12943294
Poult Sci. 2004 Jul;83(7):1093-8
pubmed: 15285498
J Dairy Res. 2009 Feb;76(1):98-104
pubmed: 19121230
J Vet Med Sci. 1995 Feb;57(1):45-9
pubmed: 7756423
Inflamm Bowel Dis. 2009 Feb;15(2):275-83
pubmed: 18839424
Avian Dis. 2008 Mar;52(1):14-22
pubmed: 18459290
Science. 2005 Mar 25;307(5717):1915-20
pubmed: 15790844
J Am Coll Nutr. 2007 Oct;26(5):445-52
pubmed: 17914132
J Nutr. 2005 Feb;135(2):187-92
pubmed: 15671211
PLoS One. 2011;6(11):e27949
pubmed: 22114729
Vet Microbiol. 2008 Jan 1;126(1-3):225-33
pubmed: 17681719
Oncotarget. 2017 Feb 7;8(6):9267-9279
pubmed: 28030827
Nat Rev Immunol. 2013 May;13(5):321-35
pubmed: 23618829
Trends Microbiol. 2004 Dec;12(12):562-8
pubmed: 15539116
Curr Opin Biotechnol. 2013 Apr;24(2):160-8
pubmed: 22940212
Int J Mol Sci. 2014 Jun 04;15(6):9963-78
pubmed: 24901527
Nucleic Acids Res. 2009 Jan;37(Database issue):D141-5
pubmed: 19004872
Dev Comp Immunol. 2010 Apr;34(4):406-17
pubmed: 19945480
Poult Sci. 2013 Mar;92(3):671-83
pubmed: 23436518
Mol Biol Rep. 2012 Dec;39(12):10595-602
pubmed: 23053958
Poult Sci. 2012 Dec;91(12):3065-71
pubmed: 23155014
Nat Methods. 2010 May;7(5):335-6
pubmed: 20383131
Benef Microbes. 2012 Sep;3(3):205-10
pubmed: 22968409
FEMS Immunol Med Microbiol. 2000 Sep;29(1):47-52
pubmed: 10967260
Int J Mol Sci. 2018 Feb 03;19(2):
pubmed: 29401695
J Nutr. 2008 Nov;138(11):2111-6
pubmed: 18936206
Pharmacognosy Res. 2012 Jan;4(1):15-21
pubmed: 22224056
Br Poult Sci. 1977 Jan;18(1):85-94
pubmed: 20197
Int J Immunopathol Pharmacol. 2013 Jan-Mar;26(1):117-25
pubmed: 23527714