Localized recombination drives diversification of killing spectra for phage-derived syringacins.
Journal
The ISME journal
ISSN: 1751-7370
Titre abrégé: ISME J
Pays: England
ID NLM: 101301086
Informations de publication
Date de publication:
02 2019
02 2019
Historique:
received:
12
01
2018
accepted:
06
07
2018
revised:
16
05
2018
pubmed:
2
9
2018
medline:
12
7
2019
entrez:
2
9
2018
Statut:
ppublish
Résumé
To better understand the potential for antagonistic interactions between members of the same bacterial species, we have surveyed bacteriocin killing activity across a diverse suite of strains of the phytopathogen Pseudomonas syringae. Our data demonstrate that killing activity from phage-derived bacteriocins of P. syringae (R-type syringacins) is widespread. Despite a high overall diversity of bacteriocin activity, strains can broadly be classified into five main killing types and two main sensitivity types. Furthermore, we show that killing activity switches frequently between strains and that switches correlate with localized recombination of two genes that together encode the proteins that specify bacteriocin targeting. Lastly, we demonstrate that phage-derived bacteriocin killing activity can be swapped between strains simply through expression of these two genes in trans. Overall, our study characterizes extensive diversity of killing activity for phage-derived bacteriocins of P. syringae across strains and highlights the power of localized recombination to alter phenotypes that mediate strain interactions during evolution of natural populations and communities.
Identifiants
pubmed: 30171255
doi: 10.1038/s41396-018-0261-3
pii: 10.1038/s41396-018-0261-3
pmc: PMC6331570
doi:
Substances chimiques
Bacteriocins
0
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
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