High-Throughput Quantitative Measurement of Bacterial Attachment Kinetics on Seconds Time Scale.
Bacterial attachment
High throughput
Kinetic measurement
Method
Journal
Microbial ecology
ISSN: 1432-184X
Titre abrégé: Microb Ecol
Pays: United States
ID NLM: 7500663
Informations de publication
Date de publication:
Apr 2019
Apr 2019
Historique:
received:
15
02
2018
accepted:
29
08
2018
pubmed:
24
9
2018
medline:
27
6
2019
entrez:
24
9
2018
Statut:
ppublish
Résumé
Surface attachment is an important factor in the life of many microbial species. Late stages of attachment (i.e., mature biofilms) are rigorously studied, but data on the very early stages is scarce. The lack of robust research methods may go a long way in explaining this situation. We have developed a method that allows the rapid kinetic measurement of bacterial attachment, with seconds to minute's temporal resolution, in a high-throughput setting. The method requires the use of a commercially available microtiter plate reader capable of fluorescence measurement from the bottom, standard microtiter plates, fluorescently tagged bacteria, and a common dye. The high temporal resolution reveals nuanced, fast, and dynamic behaviors in the very early phases of attachment. To demonstrate potential applications, we tested the effect of various conditions on attachment kinetics-specie, substratum, salt concentration, and culture density. Results are in good agreement with crystal violet staining (correlation R
Identifiants
pubmed: 30244277
doi: 10.1007/s00248-018-1254-5
pii: 10.1007/s00248-018-1254-5
doi:
Types de publication
Evaluation Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
726-735Références
J Clin Microbiol. 1999 Jun;37(6):1771-6
pubmed: 10325322
Methods Enzymol. 1999;310:523-34
pubmed: 10547816
Mol Plant Microbe Interact. 2000 Nov;13(11):1243-50
pubmed: 11059491
Mol Microbiol. 2003 Jun;48(6):1511-24
pubmed: 12791135
Nat Rev Microbiol. 2004 Feb;2(2):95-108
pubmed: 15040259
Appl Environ Microbiol. 1982 Jun;43(6):1256-61
pubmed: 16346026
Biosens Bioelectron. 2007 Jan 15;22(6):948-55
pubmed: 16697635
Int J Food Microbiol. 2006 Jul 1;110(1):62-8
pubmed: 16713644
FEMS Microbiol Lett. 2006 Aug;261(1):1-11
pubmed: 16842351
J Microbiol Methods. 2007 Mar;68(3):605-12
pubmed: 17218029
J Microbiol Methods. 2008 Feb;72(2):157-65
pubmed: 18155789
FEMS Microbiol Lett. 2008 Jun;283(1):83-90
pubmed: 18422628
Environ Sci Technol. 2009 Apr 1;43(7):2308-14
pubmed: 19452879
J Appl Microbiol. 2010 Apr;108(4):1303-12
pubmed: 19796124
Environ Sci Pollut Res Int. 2010 Nov;17(9):1529-38
pubmed: 20509051
FEMS Microbiol Ecol. 2011 Jan;75(1):17-27
pubmed: 21091520
J Vis Exp. 2011 Jan 30;(47):null
pubmed: 21307833
Langmuir. 2012 Apr 17;28(15):6396-402
pubmed: 22439703
Ann Ig. 2013 Jan-Feb;25(1):31-42
pubmed: 23435778
Biomed Res Int. 2014;2014:845147
pubmed: 24883330
Res Microbiol. 2015 Jul-Aug;166(6):535-45
pubmed: 25981538
Biosensors (Basel). 2015 May 26;5(2):276-87
pubmed: 26018780
J Microbiol Methods. 2015 Nov;118:37-41
pubmed: 26304835
Sci Rep. 2015 Nov 20;5:16857
pubmed: 26585552
Microb Ecol. 2018 Jul;76(1):9-18
pubmed: 26879941
NPJ Biofilms Microbiomes. 2016 Oct 19;2:16023
pubmed: 28721252
FEMS Microbiol Ecol. 2017 Sep 1;93(9):
pubmed: 28961787