Extremely stable indole-3-glycerol-phosphate synthase from hyperthermophilic archaeon Pyrococcus furiosus.
Gene expression
Indole-3-glycerol phosphate synthase
Pyrococcus furiosus
Thermostability
TrpC
Journal
Extremophiles : life under extreme conditions
ISSN: 1433-4909
Titre abrégé: Extremophiles
Pays: Germany
ID NLM: 9706854
Informations de publication
Date de publication:
Jan 2019
Jan 2019
Historique:
received:
21
05
2018
accepted:
24
09
2018
pubmed:
29
9
2018
medline:
2
5
2019
entrez:
29
9
2018
Statut:
ppublish
Résumé
The gene-encoding Indole-3-glycerol phosphate synthase, a key enzyme involved in the cyclization of 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate, from Pyrococcus furiosus was cloned and expressed in Escherichia coli. The gene product was produced in the soluble and active form. The recombinant protein, purified to apparent homogeneity, displayed highest activity at 100 °C and pH of 5.5. The recombinant enzyme followed Michaelis-Menten kinetics exhibiting apparent V
Identifiants
pubmed: 30264228
doi: 10.1007/s00792-018-1061-4
pii: 10.1007/s00792-018-1061-4
doi:
Substances chimiques
Archaeal Proteins
0
Indole-3-Glycerol-Phosphate Synthase
EC 4.1.1.48
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
69-77Références
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