Isolation and characterization of a potentially novel Siphoviridae phage (vB_SsapS-104) with lytic activity against Staphylococcus saprophyticus isolated from urinary tract infection.
Anti-Bacterial Agents
/ pharmacology
DNA, Viral
Genome, Viral
Host Specificity
Humans
Hydrogen-Ion Concentration
Iran
Microscopy, Electron, Transmission
Phage Therapy
Sequence Analysis, DNA
Siphoviridae
/ genetics
Staphylococcal Infections
/ microbiology
Staphylococcus Phages
/ genetics
Staphylococcus saprophyticus
/ drug effects
Temperature
Urinary Tract Infections
/ microbiology
Virulence
Virus Latency
Wastewater
/ virology
Lytic bacteriophage
Siphoviridae
Staphylococcus saprophyticus
Urinary tract infection
Journal
Folia microbiologica
ISSN: 1874-9356
Titre abrégé: Folia Microbiol (Praha)
Pays: United States
ID NLM: 0376757
Informations de publication
Date de publication:
May 2019
May 2019
Historique:
received:
13
02
2018
accepted:
25
09
2018
pubmed:
5
10
2018
medline:
19
9
2019
entrez:
5
10
2018
Statut:
ppublish
Résumé
Antibiotic resistance is increasing among Staphylococcus saprophyticus strains isolated from urinary tract infection. This necessitates alternative therapies. For this, a lytic phage (vB_SsapS-104) against S. saprophyticus, which formed round and clear plaques on bacterial culture plates, was isolated from hospital wastewater and characterized. Microscopy analysis showed that it had a small head (about 50 nm), tail (about 80 nm), and a collar (about 22 nm in length and 19 nm in width) indicating to be a phage within Siphoviridae family. Phage vB_SsapS-104 showed a large latency period of about 40 min, rapid adsorption rate that was significantly enhanced by MgCl
Identifiants
pubmed: 30284669
doi: 10.1007/s12223-018-0653-9
pii: 10.1007/s12223-018-0653-9
doi:
Substances chimiques
Anti-Bacterial Agents
0
DNA, Viral
0
Waste Water
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
283-294Subventions
Organisme : Golestan University of Medical Sciences
ID : 35/219525
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