Aseptic production of citric and isocitric acid from crude glycerol by genetically modified Yarrowia lipolytica.

Citric Acid / metabolism Glycerol / metabolism Isocitrates / metabolism Yarrowia / metabolism
Aseptic production Citric acid Crude glycerol Isocitric acid Yarrowia lipolytica

Journal

Bioresource technology
ISSN: 1873-2976
Titre abrégé: Bioresour Technol
Pays: England
ID NLM: 9889523

Informations de publication

Date de publication:
Jan 2019
Historique:
received: 20 08 2018
revised: 21 09 2018
accepted: 22 09 2018
pubmed: 7 10 2018
medline: 10 5 2019
entrez: 7 10 2018
Statut: ppublish

Résumé

The unconventional yeast Yarrowia lipolytica is known for its capacity to produce citric or isocitric acid from glycerol. In this study a reduction of production cost was achieved by using cheap crude glycerol and conducting the production at pH 3 to prevent bacterial contamination. In this study a Y. lipolytica strain overexpressing Gut1 and Gut2 was used. For the modified strain, crude glycerol proved to be an excellent substrate for production of citric/isocitric acids in aseptic conditions, as the final concentration of these compounds reached 75.9 ± 1.8 g L

Identifiants

DOI: 10.1016/j.biortech.2018.09.118 PMID: 30292133
pubmed: 30292133
pii: S0960-8524(18)31371-3
doi: 10.1016/j.biortech.2018.09.118
pii:
doi:

Substances chimiques

Isocitrates 0
Citric Acid 2968PHW8QP
isocitric acid 9RW6G5D4MQ
Glycerol PDC6A3C0OX

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

340-344

Informations de copyright

Copyright © 2018 Elsevier Ltd. All rights reserved.

Auteurs

Dorota A Rzechonek (DA)

Department of Biotechnology and Food Microbiology, Wroclaw University of Environmental and Life Sciences, Chełmońskiego 37, 51-630 Wrocław, Poland.

Adam Dobrowolski (A)

Department of Biotechnology and Food Microbiology, Wroclaw University of Environmental and Life Sciences, Chełmońskiego 37, 51-630 Wrocław, Poland.

Waldemar Rymowicz (W)

Department of Biotechnology and Food Microbiology, Wroclaw University of Environmental and Life Sciences, Chełmońskiego 37, 51-630 Wrocław, Poland.

Aleksandra M Mirończuk (AM)

Department of Biotechnology and Food Microbiology, Wroclaw University of Environmental and Life Sciences, Chełmońskiego 37, 51-630 Wrocław, Poland. Electronic address: aleksandra.mironczuk@upwr.edu.pl.

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Classifications MeSH

questionsmedicales.fr › Composés chimiques organiques › Acides carboxyliques › Acides acycliques › Triacides carboxyliques › Citrates › Acide citrique › Aseptic production of citric and isocitric...
questionsmedicales.fr › Glucides › Polyols › Dérivés du glycérol › Glycérol › Aseptic production of citric and isocitric...
questionsmedicales.fr › Composés chimiques organiques › Acides carboxyliques › Acides acycliques › Triacides carboxyliques › Citrates › Isocitrates › Aseptic production of citric and isocitric...
questionsmedicales.fr › Eucaryotes › Champignons › Levures › Yarrowia › Aseptic production of citric and isocitric...