In vitro study of the treatment of dentin hypersensitivity with gallic acid combined with sodium fluoride.
Combined effect
Dentin hypersensitivity
Gallic acid
Sodium fluoride
Journal
BMC oral health
ISSN: 1472-6831
Titre abrégé: BMC Oral Health
Pays: England
ID NLM: 101088684
Informations de publication
Date de publication:
30 Oct 2024
30 Oct 2024
Historique:
received:
22
04
2024
accepted:
23
10
2024
medline:
31
10
2024
pubmed:
31
10
2024
entrez:
31
10
2024
Statut:
epublish
Résumé
Dentin hypersensitivity (DH) is a common oral condition that is associated with severe dental pain. Pain relief is a key focus of the treatment of DH. The purpose of this study was to evaluate the blocking and antacid effects of gallic acid (GA) combined with sodium fluoride (NaF) on dentinal tubules in vitro. Ninety dentin discs from human third molars were treated with 6% citric acid for 2 min. Then, the surface morphologies of ten dentin discs were observed by scanning electron microscopy (SEM). The remaining samples were randomly divided into four groups: the NaF group, which was treated with 1000 ppm NaF; the GA group, which was treated with 4000 ppm GA; the GA + NaF group, which was treated with 1000 ppm NaF + 4000 ppm GA; and the blank group, which was treated with deionized water. The dentin permeability of each sample was measured with a water-filled system before processing and after 7 days of treatment. Dentin morphology and surface deposits were observed by SEM. Then, samples from the NaF, GA + NaF and blank groups were subjected to an acid challenge by incubation with 0.02% citric acid for 2 min. SEM and a water-filled system were used to evaluate the blocking and antacid effects of NaF and GA + NaF. 1. NaF and GA + NaF significantly decreased dentin permeability. The effect of the GA + NaF treatment was more significant. After acid challenge, both groups still exhibited decreased dentin permeability compared with the initial assessment. 2. Compared with the NaF group, the GA + NaF group had more mineral deposits on the dentin surface and dentin tubules. After acid challenge, the deposits in the GA + NaF group were still clearly visible. The combined effect of GA and NaF on reducing dentin permeability by blocking open dentin tubules is better than that of NaF alone. After acid challenge, the GA + NaF treatment still had a better effect.
Sections du résumé
BACKGROUND
BACKGROUND
Dentin hypersensitivity (DH) is a common oral condition that is associated with severe dental pain. Pain relief is a key focus of the treatment of DH. The purpose of this study was to evaluate the blocking and antacid effects of gallic acid (GA) combined with sodium fluoride (NaF) on dentinal tubules in vitro.
METHODS
METHODS
Ninety dentin discs from human third molars were treated with 6% citric acid for 2 min. Then, the surface morphologies of ten dentin discs were observed by scanning electron microscopy (SEM). The remaining samples were randomly divided into four groups: the NaF group, which was treated with 1000 ppm NaF; the GA group, which was treated with 4000 ppm GA; the GA + NaF group, which was treated with 1000 ppm NaF + 4000 ppm GA; and the blank group, which was treated with deionized water. The dentin permeability of each sample was measured with a water-filled system before processing and after 7 days of treatment. Dentin morphology and surface deposits were observed by SEM. Then, samples from the NaF, GA + NaF and blank groups were subjected to an acid challenge by incubation with 0.02% citric acid for 2 min. SEM and a water-filled system were used to evaluate the blocking and antacid effects of NaF and GA + NaF.
RESULTS
RESULTS
1. NaF and GA + NaF significantly decreased dentin permeability. The effect of the GA + NaF treatment was more significant. After acid challenge, both groups still exhibited decreased dentin permeability compared with the initial assessment. 2. Compared with the NaF group, the GA + NaF group had more mineral deposits on the dentin surface and dentin tubules. After acid challenge, the deposits in the GA + NaF group were still clearly visible.
CONCLUSION
CONCLUSIONS
The combined effect of GA and NaF on reducing dentin permeability by blocking open dentin tubules is better than that of NaF alone. After acid challenge, the GA + NaF treatment still had a better effect.
Identifiants
pubmed: 39478528
doi: 10.1186/s12903-024-05098-5
pii: 10.1186/s12903-024-05098-5
doi:
Substances chimiques
Sodium Fluoride
8ZYQ1474W7
Gallic Acid
632XD903SP
Citric Acid
2968PHW8QP
Dentin Desensitizing Agents
0
Types de publication
Journal Article
Comparative Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
1319Informations de copyright
© 2024. The Author(s).
Références
Canadian Advisory Board on Dentin H. Consensus-based recommendations for the diagnosis and management of dentin hypersensitivity. J Can Dent Assoc. 2003;69(4):221–6.
Brännström M. Sensitivity of dentine. Oral surgery, oral medicine. Oral Pathol. 1966;21(4):517–26.
doi: 10.1016/0030-4220(66)90411-7
Brännström M, Linden LA, Aström A. The hydrodynamics of the dental tubule and of pulp fluid. A discussion of its significance in relation to dentinal sensitivity. Caries Res. 1967;1(4):310–7.
pubmed: 5241870
doi: 10.1159/000259530
Pashley DH. Dentin permeability, dentin sensitivity, and treatment through tubule occlusion. J Endod. 1986;12(10):465–74.
pubmed: 3465852
doi: 10.1016/S0099-2399(86)80201-1
Li Y, Chen J, Duan Y, Zhou Z. Sealing effects of different Chinese herbal medicines on dentinal tubules: a scanning electron microscopic observation. Ultrastruct Pathol. 2020;44(3):255–61.
pubmed: 32241201
doi: 10.1080/01913123.2020.1749198
Cho J, Kim H, Yoo KH, Paik Y, Kim IR, Yoon SY, Kim YI. The effect of kaempferol on the dentin bonding stability through matrix metalloproteinases inhibition and collagen crosslink in dentin biomodification. J Dent Sci. 2023;18(3):1023–30.
pubmed: 37404650
doi: 10.1016/j.jds.2022.12.002
Murugesan S, Kumar P, Reddy BN, Arumugam K, Mohankumar P, Chandrasekaran K. Novel Management of hypersensitive dentin using Propolis-based Herbal Desensitizing agents: an in Vitro scanning Electron microscopic study. J Contemp Dent Pract. 2021;22(9):1030–4.
pubmed: 35000948
doi: 10.5005/jp-journals-10024-3154
AlQahtani SM, Nagate RR, Al-Ahmari MMM, Magbol MA, Gokhale ST, Tikare S, Chaturvedi S. Evaluation of Propolis Hydrogel for the treatment of Dentinal Sensitivity: a clinical study. Gels. 2023;9(6):483.
pubmed: 37367153
pmcid: 10298203
doi: 10.3390/gels9060483
Naghsh N, Mazrooei F, Hosseini A, Kiani S, Sahebkar A. Effects of Propolis-based Herbal Toothpaste on Dentine Hypersensitivity. Int Dent J. 2024;74(3):559–65.
pubmed: 38184459
pmcid: 11123535
doi: 10.1016/j.identj.2023.11.014
Cheng L, Li J, Hao Y, Zhou X. Effect of compounds of Galla Chinensis and their combined effects with fluoride on remineralization of initial enamel lesion in vitro. J Dent. 2008;36(5):369–73.
pubmed: 18308448
doi: 10.1016/j.jdent.2008.01.011
Cheng L, Li JY, Huang S, Zhou XD. Effect of Galla Chinensis on enhancing remineralization of enamel crystals. Biomed Mater. 2009;4(3):034103.
pubmed: 19498222
doi: 10.1088/1748-6041/4/3/034103
Chu JP, Li JY, Hao YQ, Zhou XD. Effect of compounds of Galla Chinensis on remineralisation of initial enamel carious lesions in vitro. J Dent. 2007;35(5):383–7.
pubmed: 17196320
doi: 10.1016/j.jdent.2006.11.007
Tsiogkas SG, Apostolopoulou K, Mavropoulos A, Grammatikopoulou MG, Dardiotis E, Zafiriou E, Bogdanos DP. Gallic acid diminishes pro-inflammatory interferon-γ- and interleukin-17-producing sub-populations in vitro in patients with psoriasis. Immunol Res. 2023;71(3):475–87.
pubmed: 36754913
pmcid: 10185625
doi: 10.1007/s12026-023-09361-9
Kang J, Jie L, Lu G, Fu H, Liao T, Liu D, Shi L, Yin S, Zhang L, Wang P. Gallic acid ameliorates synovial inflammation and fibrosis by regulating the intestinal flora and its metabolites. Toxicol Appl Pharmacol. 2024;490:117033.
pubmed: 38997070
doi: 10.1016/j.taap.2024.117033
Seo CS, Jeong SJ, Yoo SR, Lee NR, Shin HK. Quantitative analysis and in vitro anti-inflammatory effects of gallic acid, Ellagic Acid, and Quercetin from Radix Sanguisorbae. Pharmacogn Mag. 2016;12(46):104–8.
pubmed: 27076745
pmcid: 4809163
doi: 10.4103/0973-1296.177908
Cai L, Wei Z, Zhao X, Li Y, Li X, Jiang X. Gallic acid mitigates LPS-induced inflammatory response via suppressing NF-κB signalling pathway in IPEC-J2 cells. J Anim Physiol Anim Nutr (Berl). 2022;106(5):1000–8.
pubmed: 34288130
doi: 10.1111/jpn.13612
Jiang Y, Pei J, Zheng Y, Miao YJ, Duan BZ, Huang LF. Gallic acid: a potential Anti-cancer Agent. Chin J Integr Med. 2022;28(7):661–71.
pubmed: 34755289
doi: 10.1007/s11655-021-3345-2
Gao SS, Qian LM, Huang SB, Yu HY. Effect of gallic acid on the wear behavior of early carious enamel. Biomed Mater. 2009;4(3):034101.
pubmed: 19498226
doi: 10.1088/1748-6041/4/3/034101
Prajatelistia E, Ju SW, Sanandiya ND, Jun SH, Ahn JS, Hwang DS. Tunicate-inspired gallic acid/metal ion complex for instant and efficient treatment of dentin hypersensitivity. Adv Healthc Mater. 2016;5(8):919–27.
pubmed: 26867019
doi: 10.1002/adhm.201500878
Greenhill JD, Pashley DH. The effects of desensitizing agents on the hydraulic conductance of human dentin in vitro. J Dent Res. 1981;60(3):686–98.
pubmed: 6937499
doi: 10.1177/00220345810600030401
Itota T, Torii Y, Nakabo S, Tashiro Y, Konishi N, Nagamine M, Yoshiyama M. Effect of fluoride-releasing adhesive system on decalcified dentin. J Oral Rehabil. 2003;30(2):178–83.
pubmed: 12535145
doi: 10.1046/j.1365-2842.2003.00985.x
Kulal R, Jayanti I, Sambashivaiah S, Bilchodmath S. An invitro comparison of nano hydroxyapatite, novamin and proargin desensitizing toothpastes-a SEM study. J Clin Diagn Res. 2016;10(10):ZC51–4.
pubmed: 27891458
pmcid: 5121804
Gholami GA, Fekrazad R, Esmaiel-Nejad A, Kalhori KA. An evaluation of the occluding effects of Er; cr: YSGG, nd: YAG, CO₂ and diode lasers on dentinal tubules: a scanning electron microscope in vitro study. Photomed Laser Surg. 2011;29(2):115–21.
pubmed: 21288081
doi: 10.1089/pho.2009.2628
Santiago SL, Pereira JC, Martineli AC. Effect of commercially available and experimental potassium oxalate-based dentin desensitizing agents in dentin permeability: influence of time and filtration system. Braz Dent J. 2006;17(4):300–5.
pubmed: 17262143
doi: 10.1590/S0103-64402006000400007
Pashley DH, Galloway SE. The effects of oxalate treatment on the smear layer of ground surfaces of human dentine. Arch Oral Biol. 1985;30(10):731–7.
pubmed: 3866520
doi: 10.1016/0003-9969(85)90185-2
Wang Z, Sa Y, Sauro S, Chen H, Xing W, Ma X, Jiang T, Wang Y. Effect of desensitising toothpastes on dentinal tubule occlusion: a dentine permeability measurement and SEM in vitro study. J Dent. 2010;38(5):400–10.
pubmed: 20097250
doi: 10.1016/j.jdent.2010.01.007
Pereira JC, Segala AD, Gillam DG. Effect of desensitizing agents on the hydraulic conductance of human dentin subjected to different surface pre-treatments-an in vitro study. Dent Mater. 2005;21(2):129–38.
pubmed: 15681011
doi: 10.1016/j.dental.2004.02.007
Sauro S, Gandolfi MG, Prati C, Mongiorgi R. Oxalate-containing phytocomplexes as dentine desensitisers: an in vitro study. Arch Oral Biol. 2006;51(8):655–64.
pubmed: 16603120
doi: 10.1016/j.archoralbio.2006.02.010
Matthews B, Vongsavan N. Interactions between neural and hydrodynamic mechanisms in dentine and pulp. Arch Oral Biol. 1994;39(Suppl):S87–95.
doi: 10.1016/0003-9969(94)90193-7
Wylie SG, Wilson PR. An investigation into the pressure transmitted to the Pulp Chamber on Crown Cementation: a Laboratory Study. J Dent Res. 1994;73(11):1684–9.
pubmed: 7983254
doi: 10.1177/00220345940730110301
Kim SY, Kim EJ, Kim DS, Lee IB. The evaluation of dentinal tubule occlusion by desensitizing agents: a real-time measurement of dentinal fluid flow rate and scanning electron microscopy. Oper Dent. 2013;38(4):419–28.
pubmed: 23110582
doi: 10.2341/11-504-L
Kim MH, Kim RJ, Lee WC, Lee IB. Evaluation of dentin tubule occlusion after laser irradiation and desensitizing agent application. Am J Dent. 2015;28(5):303–8.
pubmed: 26714349
Pashley DH. Mechanisms of dentin sensitivity. Dent Clin North Am. 1990;34(3):449–73.
pubmed: 2197121
doi: 10.1016/S0011-8532(22)01128-4
Merchant V, Livingston M, Pashley D. Dentin permeation: comparison of diffusion with filtration. J Dent Res. 1977;56(10):1161–4.
pubmed: 272373
doi: 10.1177/00220345770560100601
Sauro S, Pashley DH, Montanari M, Chersoni S, Carvalho RM, Toledano M, Osorio R, Tay FR, Prati C. Effect of simulated pulpal pressure on dentin permeability and adhesion of self-etch adhesives. Dent Mater. 2007;23(6):705–13.
pubmed: 16904175
doi: 10.1016/j.dental.2006.06.010
Hiraishi N, Yiu CK, King NM, Tay FR. Effect of pulpal pressure on the microtensile bond strength of luting resin cements to human dentin. Dent Mater. 2009;25(1):58–66.
pubmed: 18573523
doi: 10.1016/j.dental.2008.05.005
Sauro S, Mannocci F, Toledano M, Osorio R, Thompson I, Watson TF. Influence of the hydrostatic pulpal pressure on droplets formation in current etch-and-rinse and self-etch adhesives: a video rate/TSM microscopy and fluid filtration study. Dent Mater. 2009;25(11):1392–402.
pubmed: 19632714
doi: 10.1016/j.dental.2009.06.010
Agee K, Zhang Y, Pashley DH. Effects of acids and additives on the susceptibility of human dentine to denaturation. J Oral Rehabil. 2000;27(2):136–41.
pubmed: 10672150
doi: 10.1046/j.1365-2842.2000.00492.x
Okamoto Y, Heeley JD, Dogon IL, Shintani H. Effects of phosphoric acid and tannic acid on dentine collagen. J Oral Rehabil. 1991;18(6):507–12.
pubmed: 1762024
doi: 10.1111/j.1365-2842.1991.tb00073.x
Jiang Dxun, Zhang M hua, Zhang Q, Chen Y, shan, Ma W, jing, Wu W, peng et al. Infuence of gallic acid on porcine neutro phils phosphodiesterase 4, IL-6, TNF-α and rat arthritis model. J Integr Agric [Internet]. Chinese Academy of Agricultural Sci ences; 2015;14:758–64.
Lin Y, Luo T, Weng A, Huang X, Yao Y, Fu Z, Li Y, Liu A, Li X, Chen D, Pan H. Gallic acid alleviates gouty arthritis by inhibiting NLRP3 inflammasome activation and pyroptosis through enhancing Nrf2 signaling. Front Immunol. 2020;11:580593.
pubmed: 33365024
pmcid: 7750458
doi: 10.3389/fimmu.2020.580593
Chu C, Ru H, Chen Y, Xu J, Wang C, Jin Y. Gallic acid attenuates LPS-induced inflammation in Caco-2 cells by suppressing the activation of the NF-κB/MAPK signaling pathway. Acta Biochim Biophys Sin (Shanghai). 2024;56(6):905–15.
pubmed: 38516705
Zamudio-Cuevas Y, Andonegui-Elguera MA, Aparicio-Juárez A, Aguillón-Solís E, Martínez-Flores K, Ruvalcaba-Paredes E, Velasquillo-Martínez C, Ibarra C, Martínez-López V, Gutiérrez M, García-Arrazola R, Hernández-Valencia CG, Romero-Montero A, Hernández-Valdepeña MA, Gimeno M, Sánchez-Sánchez R. The enzymatic poly (gallic acid) reduces pro-inflammatory cytokines in vitro, a potential application in inflammatory diseases. Inflammation. 2021;44(1):174–85.
pubmed: 32803665
doi: 10.1007/s10753-020-01319-5
Zhou G, Kong WS, Li ZC, Xie RF, Yu TY, Zhou X. Effects of Qing Chang Suppository Powder and its ingredients on IL-17 Signal pathway in HT-29 cells and DSS-induced mice. Phytomedicine. 2021;87:153573.
pubmed: 34052543
doi: 10.1016/j.phymed.2021.153573
Zhang L, Xue J, Li J, Zou L, Hao Y, Zhou X, Li W. Effects of Galla Chinensis on inhibition of demineralization of regular bovine enamel or enamel disposed of organic matrix. Arch Oral Biol. 2009;54(9):817–22.
pubmed: 19616770
doi: 10.1016/j.archoralbio.2009.06.007
Parisay I, Boskabady M, Bagheri H, Babazadeh S, Hoseinzadeh M, Esmaeilzadeh F. Investigating the efficacy of a varnish containing gallic acid on remineralization of enamel lesions: an in vitro study. BMC Oral Health. 2024;24(1):175.
pubmed: 38308290
pmcid: 10837966
doi: 10.1186/s12903-024-03921-7
Tekin GG, Deveci B. Effects of gallic acid on gingival wounds. Eur Rev Med Pharmacol Sci. 2023;27(7):2739–44.
pubmed: 37070872
Sauro S, Watson TF, Thompson I. Dentine desensitization induced by prophylactic and air-polishing procedures: an in vitro dentine permeability and confocal microscopy study. J Dent. 2010;38(5):411–22.
pubmed: 20132859
doi: 10.1016/j.jdent.2010.01.010