Influence of the terminal left domain on horizontal and vertical transmissions of tomato planta macho viroid and potato spindle tuber viroid through pollen.
Horizontal transmission
Pathogenicity domain
Pollen transmission
Potato spindle tuber viroid
Terminal left domain
Tomato planta macho viroid
Vertical transmission
Journal
Virology
ISSN: 1096-0341
Titre abrégé: Virology
Pays: United States
ID NLM: 0110674
Informations de publication
Date de publication:
02 01 2019
02 01 2019
Historique:
received:
11
06
2018
revised:
25
09
2018
accepted:
25
09
2018
pubmed:
15
10
2018
medline:
30
1
2019
entrez:
15
10
2018
Statut:
ppublish
Résumé
Viroids can be transmitted vertically and/or horizontally by pollen. Tomato planta macho viroid (TPMVd) has a high rate of horizontal transmission by pollen, whereas potato spindle tuber viroid (PSTVd) does not. To specify the domain(s) involved in horizontal transmission, four viroid chimeras were created by exchanging the terminal left (TL) and/or pathogenicity (P) domains between PSTVd and TPMVd. PSTVd-based chimeras containing TPMVd-TL and P, or TPMVd-TL alone, displayed a high rate of horizontal transmission. TPMVd-based chimeras containing PSTVd-TL and P lost infectivity, and those containing PSTVd-TL alone displayed a low rate of horizontal transmission. In addition, the vertical transmission rate was also higher in the mutants containing TPMVd-TL than in the others. These findings indicate that the sequences or structures in the TL and P (although the role is limited) domains are important not only for horizontal but also for vertical transmission by pollen.
Identifiants
pubmed: 30317103
pii: S0042-6822(18)30300-3
doi: 10.1016/j.virol.2018.09.021
pii:
doi:
Substances chimiques
RNA, Viral
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
22-31Informations de copyright
Copyright © 2018 Elsevier Inc. All rights reserved.