Conditions for Analysis of Native Protein Structures Using Uniform Field Drift Tube Ion Mobility Mass Spectrometry and Characterization of Stable Calibrants for TWIM-MS.
Calibration
Carbonic Anhydrases
/ analysis
Cytochromes c
/ analysis
Ion Mobility Spectrometry
/ methods
Myoglobin
/ analysis
Phospholipases A2
/ analysis
Protein Subunits
Proteins
/ analysis
Serum Albumin, Human
/ analysis
Solvents
/ chemistry
Spectrometry, Mass, Electrospray Ionization
/ instrumentation
Ubiquitin
/ analysis
Drift tube ion mobility mass spectrometry
Native mass spectrometry
Travelling wave ion mobility mass spectrometry
Journal
Journal of the American Society for Mass Spectrometry
ISSN: 1879-1123
Titre abrégé: J Am Soc Mass Spectrom
Pays: United States
ID NLM: 9010412
Informations de publication
Date de publication:
Feb 2019
Feb 2019
Historique:
received:
05
08
2018
accepted:
20
09
2018
revised:
14
09
2018
pubmed:
17
10
2018
medline:
15
5
2019
entrez:
17
10
2018
Statut:
ppublish
Résumé
Determination of collisional cross sections (CCS) by travelling wave ion mobility mass spectrometry (TWIM-MS) requires calibration against standards for which the CCS has been measured previously by drift tube ion mobility mass spectrometry (DTIM-MS). The different extents of collisional activation in TWIM-MS and DTIM-MS can give rise to discrepancies in the CCS of calibrants across the two platforms. Furthermore, the conditions required to ionize and transmit large, folded proteins and assemblies may variably affect the structure of the calibrants and analytes. Stable hetero-oligomeric phospholipase A
Identifiants
pubmed: 30324262
doi: 10.1007/s13361-018-2074-z
pii: 10.1007/s13361-018-2074-z
doi:
Substances chimiques
Myoglobin
0
Protein Subunits
0
Proteins
0
Solvents
0
Ubiquitin
0
Cytochromes c
9007-43-6
Phospholipases A2
EC 3.1.1.4
Carbonic Anhydrases
EC 4.2.1.1
Serum Albumin, Human
ZIF514RVZR
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
256-267Subventions
Organisme : Australian Research Council
ID : LE0882289
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