Development of a fluvoxamine detection system using a Quenchbody, a novel fluorescent biosensor.
drug screening
fluorescent biosensor
fluvoxamine
immunoassay
recombinant antibody
Journal
Drug testing and analysis
ISSN: 1942-7611
Titre abrégé: Drug Test Anal
Pays: England
ID NLM: 101483449
Informations de publication
Date de publication:
Apr 2019
Apr 2019
Historique:
received:
14
06
2018
revised:
12
10
2018
accepted:
13
10
2018
pubmed:
18
10
2018
medline:
5
11
2019
entrez:
18
10
2018
Statut:
ppublish
Résumé
The misuse of psychotropic drugs intended for medical treatment represents a recent worldwide public health concern. Quenchbody (Q-body) is a novel fluoroimmunosensor that can detect an antigen immediately without additional reagents or washing steps. Here, we describe creating Q-bodies for the detection of the antidepressant fluvoxamine (FLV) and determining optimal conditions to achieve the highest fluorescence intensity (FI). We prepared five Q-bodies with the fluorophore labeled at either the N- or C- terminus and with different linker lengths. Fluorescence was measurable within minutes, indicating the interaction of Q-bodies with FLV. The normalized FI (FI ratio) of the N-terminus labeled Q-body increased approximately 1.5-fold upon FLV addition; Q-bodies labeled at the C-terminus did not significantly increase FI. Among the fluorescence dyes used in this study, Rhodamine 6G labeled Q-body showed the best FI ratio. EC
Substances chimiques
Antidepressive Agents, Second-Generation
0
Fluorescent Dyes
0
Immunoconjugates
0
Rhodamines
0
Serotonin Uptake Inhibitors
0
rhodamine 6G
037VRW83CF
Fluvoxamine
O4L1XPO44W
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
601-609Subventions
Organisme : Japan Society for the Promotion of Science
ID : JP16K09211
Organisme : Japan Society for the Promotion of Science
ID : JP24590859
Organisme : the Cooperative Research Program of "Network Joint Research Center for Materials and Devices"
ID : 20161156
Organisme : the Cooperative Research Program of "Network Joint Research Center for Materials and Devices"
ID : 20171164
Organisme : the Cooperative Research Program of "Network Joint Research Center for Materials and Devices"
ID : 20181171
Informations de copyright
© 2018 John Wiley & Sons, Ltd.