Does vacuum centrifugal concentration reduce yield or quality of nucleic acids extracted from FFPE biospecimens?
DNA
FFPE
Integrity
Quality
RNA
Sequencing
Journal
Analytical biochemistry
ISSN: 1096-0309
Titre abrégé: Anal Biochem
Pays: United States
ID NLM: 0370535
Informations de publication
Date de publication:
01 02 2019
01 02 2019
Historique:
received:
08
11
2017
revised:
11
10
2018
accepted:
17
10
2018
pubmed:
22
10
2018
medline:
28
11
2019
entrez:
22
10
2018
Statut:
ppublish
Résumé
Vacuum centrifugal (SpeedVac) concentration is commonly applied to nucleic acids extracted from formalin-fixed paraffin-embedded (FFPE) sections, but with an unknown impact. We concentrated DNA and RNA from FFPE biospecimens using different time/temperature SpeedVac combinations of up to 30 min concentration at 45 °C, then used spectrophotometry, spectrofluorometry, RIN, PERM, DV200, qRT-PCR, DIN and the Illumina FFPE QC Assay to assess the changes in quantity, purity and integrity induced by the concentration process. We found the effects of SpeedVac concentration to be inconsequential, but an aliquot of elution buffer should be concentrated for use as the blank in spectrophotometry assays.
Identifiants
pubmed: 30343041
pii: S0003-2697(18)31079-0
doi: 10.1016/j.ab.2018.10.020
pii:
doi:
Substances chimiques
Formaldehyde
1HG84L3525
RNA
63231-63-0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
16-19Informations de copyright
Copyright © 2018 Elsevier Inc. All rights reserved.