A Multiplex-PCR Method for Diagnosis of AY-Group Phytoplasmas.
AY-group phytoplasma
Detection
Direct sequencing
Multiplex-PCR
PCR
Strain-identification
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2019
2019
Historique:
entrez:
27
10
2018
pubmed:
27
10
2018
medline:
14
6
2019
Statut:
ppublish
Résumé
Polymerase chain reaction (PCR) methods using phytoplasma-specific primers are widely used to detect phytoplasmas from infected plants and insects. Here, I describe a method of multiplex-PCR to amplify nine gene fragments in PCR reactions from AY-group phytoplasmas. Strain-identification was possible after electrophoresis and direct sequencing was also possible after PCR. The combinations of primers can be easily modified, so this method could be applied to other phytoplasma strains.
Identifiants
pubmed: 30362001
doi: 10.1007/978-1-4939-8837-2_11
doi:
Substances chimiques
Plant Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM