Immune regulation by fibroblasts in tissue injury depends on uPARAP-mediated uptake of collectins.
Animals
Bleomycin
/ administration & dosage
Bronchoalveolar Lavage Fluid
/ chemistry
Endocytosis
Fibroblasts
/ immunology
Gene Expression
Humans
Immunity, Innate
Interleukin-6
/ genetics
Lectins, C-Type
/ genetics
Lung
/ immunology
Lung Injury
/ chemically induced
Lysosomes
/ immunology
Mannose Receptor
Mannose-Binding Lectin
/ genetics
Mannose-Binding Lectins
/ genetics
Membrane Glycoproteins
/ genetics
Mice
Mice, Inbred C57BL
Mice, Transgenic
Proteolysis
Pulmonary Fibrosis
/ chemically induced
Pulmonary Surfactant-Associated Protein D
/ genetics
Receptors, Cell Surface
/ genetics
Survival Analysis
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
07 01 2019
07 01 2019
Historique:
received:
07
03
2018
revised:
14
09
2018
accepted:
17
10
2018
pubmed:
28
10
2018
medline:
23
10
2019
entrez:
28
10
2018
Statut:
ppublish
Résumé
Collectins such as mannose-binding lectin (MBL) and surfactant protein D (SP-D) become temporarily deposited in extravascular compartments after tissue injury and perform immune-stimulatory or inflammation-limiting functions. However, their turnover mechanisms, necessary to prevent excessive tissue damage, are virtually unknown. In this study, we show that fibroblasts in injured tissues undertake the clearance of collectins by using the endocytic collagen receptor uPARAP. In cellular assays, several types of collectins were endocytosed in a highly specific uPARAP-dependent process, not shared by the closely related receptor MR/CD206. When introduced into dermis or bleomycin-injured lungs of mice, collectins MBL and SP-D were endocytosed and routed for lysosomal degradation by uPARAP-positive fibroblasts. Fibroblast-specific expression of uPARAP governed endogenous SP-D levels and overall survival after lung injury. In lung tissue from idiopathic pulmonary fibrosis patients, a strong up-regulation of uPARAP was observed in fibroblasts adjacent to regions with SP-D secretion. This study demonstrates a novel immune-regulatory function of fibroblasts and identifies uPARAP as an endocytic receptor in immunity.
Identifiants
pubmed: 30366943
pii: jcb.201802148
doi: 10.1083/jcb.201802148
pmc: PMC6314555
doi:
Substances chimiques
IL6 protein, human
0
Interleukin-6
0
Lectins, C-Type
0
MRC2 protein, human
0
Mannose Receptor
0
Mannose-Binding Lectin
0
Mannose-Binding Lectins
0
Membrane Glycoproteins
0
Pulmonary Surfactant-Associated Protein D
0
Receptors, Cell Surface
0
Bleomycin
11056-06-7
Types de publication
Journal Article
Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
333-349Subventions
Organisme : Intramural NIH HHS
ID : ZIC DE000729
Pays : United States
Informations de copyright
© 2018 Jürgensen et al.
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