Chromatin Immunoprecipitation in Skeletal Myoblasts.

Animals Chromatin / genetics Chromatin Immunoprecipitation Data Analysis High-Throughput Nucleotide Sequencing Histones / metabolism Myoblasts, Skeletal / metabolism Real-Time Polymerase Chain Reaction
Chromatin modifiers Epigenetics Histone modifications Myoblasts Transcription

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2019
Historique:
entrez: 28 10 2018
pubmed: 28 10 2018
medline: 5 6 2019
Statut: ppublish

Résumé

Chromatin immunoprecipitation (ChIP) is a powerful and sensitive technique that is widely used to study DNA-protein interactions. It enables an unbiased genome-wide analysis of transcriptional changes during several biological processes including cellular differentiation. Here, we describe a step-by-step protocol to identify histone modifications, transcription factor, and co-factor binding to chromatin in skeletal myoblasts. We discuss critical steps during cell harvesting, sonication, and immunoprecipitation and provide notes to evade common pitfalls.

Identifiants

DOI: 10.1007/978-1-4939-8897-6_4 PMID: 30367408
pubmed: 30367408
doi: 10.1007/978-1-4939-8897-6_4
doi:

Substances chimiques

Chromatin 0
Histones 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

43-54

Auteurs

Vinay Kumar Rao (VK)

Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.
Temasek Life Sciences Laboratory, National University of Singapore, Singapore, Singapore.

Shilpa Rani Shankar (SR)

Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.

Reshma Taneja (R)

Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore. phsrt@nus.edu.sg.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chromatin Immunoprecipitation in Skeletal Myoblasts.
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Structures de chromosome › Chromatine › Chromatin Immunoprecipitation in Skeletal Myoblasts.
questionsmedicales.fr › Techniques d'investigation › Techniques immunologiques › Immunoprécipitation › Immunoprécipitation de la chromatine › Chromatin Immunoprecipitation in Skeletal Myoblasts.
questionsmedicales.fr › Disciplines des sciences naturelles › Mathématiques › Analyse de données › Chromatin Immunoprecipitation in Skeletal Myoblasts.
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Analyse de séquence › Séquençage nucléotidique à haut débit › Chromatin Immunoprecipitation in Skeletal Myoblasts.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Nucléoprotéines › Histone › Chromatin Immunoprecipitation in Skeletal Myoblasts.
questionsmedicales.fr › Cellules › Cellules souches › Myoblastes › Myoblastes squelettiques › Chromatin Immunoprecipitation in Skeletal Myoblasts.
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › Réaction de polymérisation en chaine en temps réel › Chromatin Immunoprecipitation in Skeletal Myoblasts.