Viral Genetics Modulate Orolabial Herpes Simplex Virus Type 1 Shedding in Humans.
CD4 T cells
Herpes simplex virus
cold sores
herpes labialis
twins
Journal
The Journal of infectious diseases
ISSN: 1537-6613
Titre abrégé: J Infect Dis
Pays: United States
ID NLM: 0413675
Informations de publication
Date de publication:
15 03 2019
15 03 2019
Historique:
received:
20
07
2018
accepted:
30
10
2018
pubmed:
2
11
2018
medline:
14
1
2020
entrez:
2
11
2018
Statut:
ppublish
Résumé
Orolabial herpes simplex virus type 1 (HSV-1) infection has a wide spectrum of severity in immunocompetent persons. To study the role of viral genotype and host immunity, we characterized oral HSV-1 shedding rates and host cellular response, and genotyped viral strains, in monozygotic (MZ) and dizygotic (DZ) twins. A total of 29 MZ and 22 DZ HSV-1-seropositive twin pairs were evaluated for oral HSV-1 shedding for 60 days. HSV-1 strains from twins were genotyped as identical or different. CD4+ T-cell responses to HSV-1 proteins were studied. The median per person oral HSV shedding rate was 9% of days that a swab was obtained (mean, 10.2% of days). A positive correlation between shedding rates was observed within all twin pairs, and in the MZ and DZ twins. In twin subsets with sufficient HSV-1 DNA to genotype, 15 had the same strain and 14 had different strains. Viral shedding rates were correlated for those with the same but not different strains. The median number of HSV-1 open reading frames recognized per person was 16. The agreement in the CD4+ T-cell response to specific HSV-1 open reading frames was greater between MZ twins than between unrelated persons (P = .002). Viral strain characteristics likely contribute to oral HSV-1 shedding rates.
Sections du résumé
BACKGROUND
Orolabial herpes simplex virus type 1 (HSV-1) infection has a wide spectrum of severity in immunocompetent persons. To study the role of viral genotype and host immunity, we characterized oral HSV-1 shedding rates and host cellular response, and genotyped viral strains, in monozygotic (MZ) and dizygotic (DZ) twins.
METHODS
A total of 29 MZ and 22 DZ HSV-1-seropositive twin pairs were evaluated for oral HSV-1 shedding for 60 days. HSV-1 strains from twins were genotyped as identical or different. CD4+ T-cell responses to HSV-1 proteins were studied.
RESULTS
The median per person oral HSV shedding rate was 9% of days that a swab was obtained (mean, 10.2% of days). A positive correlation between shedding rates was observed within all twin pairs, and in the MZ and DZ twins. In twin subsets with sufficient HSV-1 DNA to genotype, 15 had the same strain and 14 had different strains. Viral shedding rates were correlated for those with the same but not different strains. The median number of HSV-1 open reading frames recognized per person was 16. The agreement in the CD4+ T-cell response to specific HSV-1 open reading frames was greater between MZ twins than between unrelated persons (P = .002).
CONCLUSION
Viral strain characteristics likely contribute to oral HSV-1 shedding rates.
Identifiants
pubmed: 30383234
pii: 5154868
doi: 10.1093/infdis/jiy631
pmc: PMC6420167
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Twin Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
1058-1066Subventions
Organisme : NIAID NIH HHS
ID : R21 AI081347
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI007044
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI094019
Pays : United States
Organisme : NIAID NIH HHS
ID : P01 AI030731
Pays : United States
Organisme : NHLBI NIH HHS
ID : RC2 HL103416
Pays : United States
Informations de copyright
© The Author(s) 2018. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.
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