High-efficiency enzymatic production of α-isoquercitrin glucosides by amylosucrase from Deinococcus geothermalis.
Amylosucrase
CGTase
Deinococcus geothermalis
Enzymatically modified isoquercitrin (EMIQ)
Transglycosylation
Journal
Enzyme and microbial technology
ISSN: 1879-0909
Titre abrégé: Enzyme Microb Technol
Pays: United States
ID NLM: 8003761
Informations de publication
Date de publication:
Jan 2019
Jan 2019
Historique:
received:
21
08
2018
revised:
30
09
2018
accepted:
17
10
2018
entrez:
7
11
2018
pubmed:
7
11
2018
medline:
23
2
2019
Statut:
ppublish
Résumé
Isoquercitrin (IQ, quercetin-3-O-β-d-glucopyranoside) has diverse biological functions, such as anti-oxidant and anti-cancer activity, but its use is limited by poor solubility and bioavailability. Enzymatically modified IQ (EMIQ) is a mixture of transglycosylated IQs that have better solubility and bioavailability than do quercetin and IQ. Two different enzymes, cyclodextrin glucanotransferase (CGTase) and amylosucrase (ASase), have the transglycosylation activity to produce EMIQ. Both enzymes produce a variety of EMIQs including IQ, IQ-glucoside (IQ-G1), IQ-diglucoside (IQ-G2), and IQ-triglucoside (IQ-G3). ASase had a higher bioconversion yield from IQ to EMIQ (97.6%) than did CGTase (76.8%). In addition, the yield of IQ-G3, which was the most bioavailable form, was higher with ASase (46%) than with CGTases (8%). Taken together, these results suggest that ASase can be used to synthesize EMIQ in a simple and specific process.
Identifiants
pubmed: 30396404
pii: S0141-0229(18)30496-4
doi: 10.1016/j.enzmictec.2018.10.006
pii:
doi:
Substances chimiques
Glucosides
0
isoquercitrin
0YX10VRV6J
Quercetin
9IKM0I5T1E
Glucosyltransferases
EC 2.4.1.-
amylosucrase
EC 2.4.1.4
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
84-90Informations de copyright
Copyright © 2018 Elsevier Inc. All rights reserved.