Pentraxin 3 regulates synaptic function by inducing AMPA receptor clustering via ECM remodeling and β1-integrin.
Animals
Astrocytes
/ metabolism
Brain
/ growth & development
C-Reactive Protein
/ genetics
CHO Cells
Cells, Cultured
Cricetinae
Cricetulus
Extracellular Matrix
/ genetics
Integrin beta1
/ metabolism
Mice
Mice, Inbred C57BL
Nerve Tissue Proteins
/ genetics
Neuronal Plasticity
/ genetics
Protein Transport
/ genetics
Receptors, AMPA
/ metabolism
Synapses
/ physiology
Thrombospondin 1
/ metabolism
AMPARs
PTX3
astrocyte
synapse
thrombospondin
Journal
The EMBO journal
ISSN: 1460-2075
Titre abrégé: EMBO J
Pays: England
ID NLM: 8208664
Informations de publication
Date de publication:
03 01 2019
03 01 2019
Historique:
received:
29
03
2018
revised:
12
09
2018
accepted:
01
10
2018
pubmed:
7
11
2018
medline:
1
1
2020
entrez:
7
11
2018
Statut:
ppublish
Résumé
Control of synapse number and function in the developing central nervous system is critical to the formation of neural circuits. Astrocytes play a key role in this process by releasing factors that promote the formation of excitatory synapses. Astrocyte-secreted thrombospondins (TSPs) induce the formation of structural synapses, which however remain post-synaptically silent, suggesting that completion of early synaptogenesis may require a two-step mechanism. Here, we show that the humoral innate immune molecule Pentraxin 3 (PTX3) is expressed in the developing rodent brain. PTX3 plays a key role in promoting functionally-active CNS synapses, by increasing the surface levels and synaptic clustering of AMPA glutamate receptors. This process involves tumor necrosis factor-induced protein 6 (TSG6), remodeling of the perineuronal network, and a β1-integrin/ERK pathway. Furthermore, PTX3 activity is regulated by TSP1, which directly interacts with the N-terminal region of PTX3. These data unveil a fundamental role of PTX3 in promoting the first wave of synaptogenesis, and show that interplay of TSP1 and PTX3 sets the proper balance between synaptic growth and synapse function in the developing brain.
Identifiants
pubmed: 30396995
pii: embj.201899529
doi: 10.15252/embj.201899529
pmc: PMC6315291
pii:
doi:
Substances chimiques
Integrin beta1
0
Nerve Tissue Proteins
0
Receptors, AMPA
0
Thrombospondin 1
0
neuronal pentraxin
0
C-Reactive Protein
9007-41-4
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2018 The Authors. Published under the terms of the CC BY NC ND 4.0 license.
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