Anisotropic freeze-cast collagen scaffolds for tissue regeneration: How processing conditions affect structure and properties in the dry and fully hydrated states.
Confocal microscopy
Correlations
Longitudinal
Porosity
Radial
Journal
Journal of the mechanical behavior of biomedical materials
ISSN: 1878-0180
Titre abrégé: J Mech Behav Biomed Mater
Pays: Netherlands
ID NLM: 101322406
Informations de publication
Date de publication:
02 2019
02 2019
Historique:
received:
12
07
2018
revised:
25
08
2018
accepted:
11
09
2018
pubmed:
7
11
2018
medline:
11
2
2020
entrez:
7
11
2018
Statut:
ppublish
Résumé
Few systematic structure-property-processing correlations for directionally freeze-cast biopolymer scaffolds are reported. Such correlations are critical to enable scaffold design with attractive structural and mechanical cues in vivo. This study focuses on freeze-cast collagen scaffolds with three different applied cooling rates (10, 1, and 0.1 °C/min) and two freezing directions (longitudinal and radial). A semi-automated approach for the structural characterization of fully hydrated scaffolds by confocal microscopy is developed to facilitate an objective quantification and comparison of structural features. Additionally, scanning electron microscopy and compression testing are performed longitudinally and transversely. Structural and mechanical properties are determined on dry and fully hydrated scaffolds. Longitudinally frozen scaffolds have aligned and regular pores while those in radially frozen ones exhibit greater variations in pore geometry and alignment. Lamellar spacing, pore area, and cell wall thickness increase with decreasing cooling rate: in longitudinally frozen scaffolds from 25 µm to 83.5 µm, from 814 µm
Identifiants
pubmed: 30399564
pii: S1751-6161(18)31026-9
doi: 10.1016/j.jmbbm.2018.09.012
pmc: PMC6777344
mid: NIHMS1052502
pii:
doi:
Substances chimiques
Biocompatible Materials
0
Collagen
9007-34-5
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
350-364Subventions
Organisme : NICHD NIH HHS
ID : R21 HD087828
Pays : United States
Organisme : NIBIB NIH HHS
ID : T32 EB021966
Pays : United States
Informations de copyright
Copyright © 2018 Elsevier Ltd. All rights reserved.
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