HiPLA: High-throughput imaging proximity ligation assay.
High-throughput imaging
Hutchinson-Gilford progeria syndrome
Lamin proteomics
Proximity ligation
Journal
Methods (San Diego, Calif.)
ISSN: 1095-9130
Titre abrégé: Methods
Pays: United States
ID NLM: 9426302
Informations de publication
Date de publication:
15 03 2019
15 03 2019
Historique:
received:
12
07
2018
revised:
29
10
2018
accepted:
06
11
2018
pubmed:
13
11
2018
medline:
22
11
2019
entrez:
13
11
2018
Statut:
ppublish
Résumé
Protein-protein interactions are essential for cellular structure and function. To delineate how the intricate assembly of protein interactions contribute to cellular processes in health and disease, new methodologies that are both highly sensitive and can be applied at large scale are needed. Here, we develop HiPLA (high-throughput imaging proximity ligation assay), a method that employs the well-established antibody-based proximity ligation assay in a high-throughput imaging screening format as a novel means to systematically visualize protein interactomes. Using HiPLA with a library of antibodies targeting nuclear proteins, we probe the interaction of 60 proteins and associated post-translational modifications (PTMs) with the nuclear lamina in a model of the premature aging disorder Hutchinson-Gilford progeria syndrome (HGPS). We identify a subset of proteins that differentially interact with the nuclear lamina in HGPS. Using HiPLA in combination with quantitative indirect immunofluorescence, we find that the majority of differential interactions are accompanied by corresponding changes in expression of the interacting protein. Taken together, HiPLA offers a novel approach to probe cellular protein-protein interaction at a large scale and reveals mechanistic insights into the assembly of protein complexes.
Identifiants
pubmed: 30419336
pii: S1046-2023(18)30114-2
doi: 10.1016/j.ymeth.2018.11.004
pmc: PMC6401276
mid: NIHMS1512202
pii:
doi:
Substances chimiques
Lamin Type A
0
Protein Precursors
0
Types de publication
Journal Article
Research Support, N.I.H., Intramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
80-87Subventions
Organisme : Intramural NIH HHS
ID : ZIA BC010309-19
Pays : United States
Organisme : Intramural NIH HHS
ID : ZIA BC010309-20
Pays : United States
Informations de copyright
Published by Elsevier Inc.
Références
Nat Cell Biol. 2008 Apr;10(4):452-9
pubmed: 18311132
Methods. 2008 Jul;45(3):227-32
pubmed: 18620061
J Biomol Screen. 2014 Jan;19(1):176-83
pubmed: 23983231
Curr Biol. 2016 Oct 10;26(19):2651-2658
pubmed: 27641764
Nat Rev Mol Cell Biol. 2013 Jan;14(1):13-24
pubmed: 23212477
Methods. 2016 Mar 1;96:46-58
pubmed: 26341717
Cell. 2014 Nov 20;159(5):1212-1226
pubmed: 25416956
J Cell Biol. 2018 Jan 2;217(1):21-37
pubmed: 29051264
Sci Rep. 2015 Jul 23;5:12317
pubmed: 26202090
Methods Mol Biol. 2016;1411:147-58
pubmed: 27147040
Aging Cell. 2018 Apr;17(2):
pubmed: 29405587
Nature. 2003 May 15;423(6937):293-8
pubmed: 12714972
J Clin Invest. 2016 Oct 3;126(10):3879-3893
pubmed: 27617860
Proc Natl Acad Sci U S A. 1999 Mar 16;96(6):2852-7
pubmed: 10077600
Proteomics. 2011 May;11(10):2063-70
pubmed: 21480528
J Cell Biol. 1995 Oct;131(1):33-44
pubmed: 7559784
Mol Biol Cell. 2016 Apr 15;27(8):1188-96
pubmed: 26912792
Nat Biotechnol. 2012 Nov;30(11):1143-8
pubmed: 23086203
Mol Cell Proteomics. 2010 Jan;9(1):178-83
pubmed: 19864249
Mech Ageing Dev. 2010 Mar;131(3):185-92
pubmed: 20117128
Proc Natl Acad Sci U S A. 2005 Sep 6;102(36):12879-84
pubmed: 16129833
J Histochem Cytochem. 2008 Aug;56(8):711-21
pubmed: 18474937
Cell. 2015 Jul 16;162(2):425-440
pubmed: 26186194
J Pathol. 2012 Jan;226(2):316-25
pubmed: 21953297
PLoS One. 2010 Jun 15;5(6):e11132
pubmed: 20559568
Cell Rep. 2015 Aug 11;12(6):1019-31
pubmed: 26235622
Biomol Detect Quantif. 2015 May 20;4:10-6
pubmed: 27077033
Curr Protoc Protein Sci. 2013 Nov 05;74:19.23.1-19.23.14
pubmed: 24510646
Elife. 2015 Aug 27;4:
pubmed: 26312502
Nat Biotechnol. 2002 May;20(5):473-7
pubmed: 11981560
Cell. 2014 Jul 31;158(3):633-46
pubmed: 25083873
IEEE/ACM Trans Comput Biol Bioinform. 2015 Jan-Feb;12(1):30-9
pubmed: 26357076
FEBS Lett. 2010 Jul 16;584(14):2999-3004
pubmed: 20580717
Genes Dev. 2008 Dec 15;22(24):3409-21
pubmed: 19141474
Science. 2000 Mar 31;287(5462):2486-92
pubmed: 10741968
Proc Natl Acad Sci U S A. 2004 Jun 15;101(24):8963-8
pubmed: 15184648
Cell Rep. 2015 Nov 17;13(7):1396-1406
pubmed: 26549451
Nat Rev Mol Cell Biol. 2017 Nov;18(11):685-701
pubmed: 28875992
Nat Rev Mol Cell Biol. 2017 Oct;18(10):595-609
pubmed: 28792007
J Cell Biol. 2015 Nov 9;211(3):533-51
pubmed: 26553927
Cytometry A. 2017 Feb;91(2):180-189
pubmed: 28094900
Mol Cell Biol. 2000 Apr;20(7):2400-10
pubmed: 10713164
Histochem Cell Biol. 2016 Apr;145(4):401-17
pubmed: 26847180
Chromosoma. 2012 Oct;121(5):447-64
pubmed: 22610065
Cell Metab. 2012 Dec 5;16(6):738-50
pubmed: 23217256
Mol Biol Cell. 2014 May;25(9):1493-510
pubmed: 24623722
Nat Med. 2005 Apr;11(4):440-5
pubmed: 15750600
Proteomics. 2007 Aug;7(16):2833-42
pubmed: 17640003
Trends Biochem Sci. 2017 Jul;42(7):504-515
pubmed: 28566215
Hum Mol Genet. 2006 Apr 1;15(7):1113-22
pubmed: 16481358
Nat Methods. 2009 Aug;6(8):569-75
pubmed: 19644458
Biol Open. 2016 Aug 15;5(8):1011-21
pubmed: 27402967
Nucleus. 2010 Nov-Dec;1(6):513-25
pubmed: 21327095
PLoS One. 2013 May 02;8(5):e61893
pubmed: 23658700
Science. 2003 Jun 27;300(5628):2055
pubmed: 12702809
Anal Biochem. 2005 Oct 1;345(1):2-9
pubmed: 15950911
J Med Genet. 2004 Apr;41(4):304-8
pubmed: 15060110
Expert Rev Proteomics. 2014 Dec;11(6):733-43
pubmed: 25227871